Protective effects of resveratrol on hepatic ischemia reperfusion injury in streptozotocin-induced diabetic rats

  • Hanife Serife AktaşEmail author
  • Yahya Ozel
  • Sarfraz AhmadEmail author
  • Halime Hanim Pençe
  • Betul Ayaz-Adakul
  • Ilyas Kudas
  • Sermin Tetik
  • Turgut Şekerler
  • Ceren Canbey-Göret
  • Levent Kabasakal
  • Hatice Kubra Elcioglu


Resveratrol (RSV) is a natural polyphenolic compound having antioxidant effects. This study was designed to investigate the protective effects of resveratrol against oxidative stress in hepatic ischemia–reperfusion (I/R) injury in streptozotocin (STZ)-induced diabetic rats. STZ was injected intraperitonally (i.p.) to 18 Sprague–Dawley albino rats, which were divided into three groups, each having six rats. First group was non-treated diabetic group (D), second diabetic group was subjected to 30 min of hepatic ischemia followed by a 45-min reperfusion period (D + I/R), and third diabetic group was subjected to 30 min of hepatic ischemia followed by a 45-min reperfusion period and treated with 20 mg/kg/day oral RSV before 30 min I/R injury (D + I/R + RSV). At the end of the experimental period, animals were decapitated, and blood samples were collected to determine tissue tumor necrosis factor-α (TNF-α) levels. Liver and lung tissue samples were obtained for the evaluation of biochemical parameters including malondialdehyde (MDA) and glutathione (GSH) levels and histopathological examinations. Compared to control, I/R injury resulted in decreases in GSH levels and increases in MDA levels. Tissue TNF-α levels were also increased in the D + I/R group compared to D group. Treatment with RSV prevented the alterations on biochemical parameters and histopathological changes induced by I/R. We demonstrate that in diabetic rats, hepatic I/R injury is associated with an augmented inflammatory response and oxidative stress, while RSV pre-treatment significantly decreased these responses. Larger clinical studies are desirable to determine the exact role(s) of RSV on hepatic I/R injury among diabetic subjects.


Resveratrol Diabetes Ischemia reperfusion injury Inflammation Experimental rats 



This study was not funded from any source, except the primary institutional support.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest associated with this research manuscript.

Ethical approval

All procedures performed in this study involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Hanife Serife Aktaş
    • 1
    Email author
  • Yahya Ozel
    • 2
  • Sarfraz Ahmad
    • 3
    Email author
  • Halime Hanim Pençe
    • 4
  • Betul Ayaz-Adakul
    • 5
  • Ilyas Kudas
    • 2
  • Sermin Tetik
    • 6
  • Turgut Şekerler
    • 6
  • Ceren Canbey-Göret
    • 7
  • Levent Kabasakal
    • 5
  • Hatice Kubra Elcioglu
    • 5
  1. 1.Internal Medicine ClinicUmraniye Research and Training HospitalIstanbulTurkey
  2. 2.General Surgery ClinicUmraniye Research and Training HospitalIstanbulTurkey
  3. 3.AdventHealth Medical CenterOrlandoUSA
  4. 4.Department of Biochemistry, Faculty of MedicineHealth Sciences UniversityIstanbulTurkey
  5. 5.Department of Pharmacology, Pharmacy FacultyMarmara UniversityIstanbulTurkey
  6. 6.Department of Biochemistry, Pharmacy FacultyMarmara UniversityIstanbulTurkey
  7. 7.Department of PathologyProf. Dr İlhan Varank Sancaktepe Research and Training HospitalIstanbulTurkey

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