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The cardioprotective effects of diallyl trisulfide on diabetic rats with ex vivo induced ischemia/reperfusion injury

  • Jovana N. Jeremic
  • Vladimir Lj. Jakovljevic
  • Vladimir I. Zivkovic
  • Ivan M. Srejovic
  • Jovana V. Bradic
  • Sergey Bolevich
  • Tamara R. Nikolic Turnic
  • Slobodanka Lj. Mitrovic
  • Nemanja U. Jovicic
  • Suresh C. Tyagi
  • Nevena S. JeremicEmail author
Article

Abstract

Diallyl trisulfide (DATS) is distinguished as the most potent polysulfide isolated from garlic. The aim of our study was to investigate effects of oral administration of DATS on healthy and diabetic rats, with special attention on heart function. Rats were randomly divided into four groups: CTRL (healthy rats), DATS (healthy rats treated with DATS), DM (diabetic rats), DM + DATS (diabetic rats treated with DATS). DATS (40 mg/kg of body weight) was administered every other day for 3 weeks, at the end of which rats underwent echocardiography, glycemic measurement and redox status assessment. Isolated rat hearts were subjected to 30 min global ischemia and 60 min reperfusion, after which heart tissue was counterstain with hematoxylin and eosin and cardiac Troponin T staining (cTnT), while expression of Bax, B cell lymphoma 2 (Bcl-2), caspase-3, caspase-9 and superoxide dismutase-2 were examined in the left ventricle. DATS treatment significantly reduced blood glucose levels of diabetic rats, and improved cardiac function recovery, diminished oxidation status, attenuated cardiac remodeling and inhibited myocardial apoptosis in healthy and diabetic rats. DATS treatment causes promising cardioprotective effects on ex vivo-induced ischemia/reperfusion (I/R) injury in diabetic and healthy rat heart probably mediated by inhibited myocardial apoptosis. Moreover, appropriate DATS consumption may provide potential co-therapy or prevention of hyperglycemia and various cardiac complications in rats with DM.

Keywords

Cardioprotection Diallyl trisulfide Diabetes Ischemia–reperfusion injury Isolated heart 

Notes

Acknowledgements

This work was supported by Junior Project 03/18 by the Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.

Compliance with ethical standards

Conflict of interest

The authors have declared no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Jovana N. Jeremic
    • 1
  • Vladimir Lj. Jakovljevic
    • 2
    • 3
  • Vladimir I. Zivkovic
    • 2
  • Ivan M. Srejovic
    • 2
  • Jovana V. Bradic
    • 1
  • Sergey Bolevich
    • 3
  • Tamara R. Nikolic Turnic
    • 1
  • Slobodanka Lj. Mitrovic
    • 4
  • Nemanja U. Jovicic
    • 5
  • Suresh C. Tyagi
    • 6
  • Nevena S. Jeremic
    • 1
    Email author
  1. 1.Department of Pharmacy, Faculty of Medical SciencesUniversity of KragujevacKragujevacSerbia
  2. 2.Department of Physiology, Faculty of Medical SciencesUniversity of KragujevacKragujevacSerbia
  3. 3.Department of Human Pathology, 1st Moscow State MedicalUniversity IM SechenovMoscowRussia
  4. 4.Department of Pathology, Faculty of Medical SciencesUniversity of KragujevacKragujevacSerbia
  5. 5.Department of Histology and Embryology, Faculty of Medical SciencesUniversity of KragujevacKragujevacSerbia
  6. 6.Department of Physiology, School of MedicineUniversity of LouisvilleLouisvilleUSA

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