Ameliorating effect of lipo-ATRA treatment on the expression of TIG3 and its suppressing effect on PPARγ gene expression in lung cancer animal model
This study aimed to find out the molecular therapeutic effect of lipo-ATRA on tumour suppressor TIG3 and cell proliferative biomarker PPARγ in B (a) P-induced lung cancer model. In RT-PCR study, ATRA- and lipo-ATRA-treated mice samples showed relatively higher TIG3 expression and decreased PPARγ expression (Band density) than cancer control. Among treatments, lipo-ATRA showed vital effect than free ATRA by enhancing TIG3 and decreasing PPARγ. The qPCR results also showed significant (p ≤ 0.05) difference in both TIG3 and PPAR (RQ values of TIG3, lipo-ATRA 23.85 ± 1.29; free ATRA 10.43 ± 1.81 and for PPARγ, lipo-ATRA 4.707 ± 1.21; free ATRA 15.78 ± 2.34). From this, we conclude that liposomal ATRA formulation is most preferable for prolonged delivery of ATRA at targeted site to favour molecular action. It implies that the therapeutic effect of lipo-ATRA in lung cancer was exhibited by ameliorating the TIG3 expression and by suppressing the expression of PPARγ.
KeywordsLung cancer Lipo-ATRA Benzo (a) pyrene TIG3 PPARγ
Tazarotene-induced gene 3
Peroxisome proliferator-activated receptor gamma
Retinoic acid receptor
Retinoid X receptor
All trans retinoic acid
Retinoic acid-responsive elements
- B (a) P
Benzo (a) Pyrene
We are very grateful to thank the Department of Science and Technology-Science and Engineering Research board (DST-SERB), Govt. of India [SB/YS/LS-252/2013 (May 15, 2014)], Department of Biotechnology (DBT), Govt of India [BT/PR14632/NNT/28/824/2015] and Karunya short-term Research grant 2018–2019 [KITS/KSG/56/2018] for the financial support given to complete this work successfully. We extend our thanks to Ms. Perinba Danisha J, SRF, KITS, and Mr. P. Jeyakumar for their valuable guidance and technical assistance during this research work. We acknowledge the Karunya Institute of Technology and Sciences, Coimbatore, for providing instruments and laboratory facilities. We also thank the timely help rendered by the authorities of Sugarcane Breeding Institute, Coimbatore, to carry out the real-time qPCR for the gene expression study.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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