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LncRNA H19 promotes lung cancer proliferation and metastasis by inhibiting miR-200a function

  • Yi Zhao
  • Changjiang Feng
  • Yunjing Li
  • Yongfu MaEmail author
  • Ruijun CaiEmail author
Article
  • 45 Downloads

Abstract

Lung cancer is the major cause leading to cancer mortality, and the 5-year survival rate for patients with lung cancer still remains low. It is urgent to fully understand the development and progression of lung cancer to discover new therapeutic targets and develop new therapeutic approaches. H19 was documented to be upregulated in lung cancer and related to cell proliferation. However, it is still unclear if H19 has other functions in lung cancer. The mRNA levels of genes were detected by qRT-PCR, and the cell proliferation rate and cell viability were measured through cell count assay and MTT assay. Transwell assays were applied to detect cell abilities to migration and invasion, while luciferase reporter assay and RNA pull-down assay were used to examine interaction between H19 and miR-200a. H19 expression was elevated in the lung cancer tissues and cell lines, while H19 overexpression promoted the lung cancer cell growth, cell migration and invasion, as well as the epithelial mesenchymal transition (EMT). Meantime, RNA pull-down assay showed that H19 interacted with miR-200a, and miR-200a inhibited the activity of H19-fused luciferase. Furthermore, H19 overexpression inhibited miR-200a function and thereby upregulated miR-200a target genes, ZEB1 and ZEB2.H19 sponged and inhibited miR-200a to de-repress expression of ZEB1 and ZEB2, and thereby enhanced lung cancer proliferation and metastasis.

Keywords

H19 miR-200a Lung cancer Proliferation Metastasis 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human and/or animal rights

This study had been approved by The Human Research Ethics Committee of the Integrated Hospital of Traditional Chinese Medicine, Southern Medical University.

Informed consent

Written consents were derived from the participants.

Supplementary material

11010_2019_3564_MOESM1_ESM.pdf (98 kb)
Supplementary material 1 (PDF 97 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Thoracic Surgery, Integrated Hospital of Traditional Chinese MedicineSouthern Medical UniversityGuangzhouChina
  2. 2.Department of Thoracic SurgeryPLA General HospitalBeijingChina

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