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Heat shock protein 60 negatively regulates the biological functions of ubiquitin-like protein MNSFβ in macrophages

  • Morihiko NakamuraEmail author
  • Kaori Notsu
  • Mai Nakagawa
Article
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Abstract

Monoclonal nonspecific suppressor factor β (MNSFβ) is a ubiquitously expressed ubiquitin-like protein known to be involved in various biological functions. Previous studies have demonstrated that MNSFβ covalently modify its target proteins including Bcl-G, a proapoptotic protein. In this study, we purified a 65 kDa MNSFβ adduct from mouse liver lysates by sequential chromatography on DEAE and glutathione S-transferase (GST)-fusioned MNSFβ immobilized on glutathione-Sepharose beads in the presence of ATP. MALDI-TOF mass spectrometry fingerprinting revealed that this MNSFβ adduct consists of an 8.5 kDa MNSFβ and heat shock protein 60 (HSP60), a mitochondrial protein involved in protein folding. Fingerprinting analysis of the MNSFβ adduct demonstrates that MNSFβ conjugates to HSP60 with a linkage between the C-terminal Gly74 and Lys481. HSP60 siRNA neutralized the inhibition of apoptosis by MNSFβ siRNA in LPS/IFNγ-stimulated Raw264.7, a murine macrophage cell line. HSP60 siRNA also down-regulated the enhancement of TNFα production by MNSFβ siRNA in LPS-stimulated Raw264.7 cells. Here, we firstly report that MNSFβ activity is negatively regulated by molecular chaperone.

Keywords

Ubiquitin-like protein Molecular chaperone Inflammation responses 

Abbreviations

MNSF

Monoclonal nonspecific suppressor factor

LPS

Lipopolysaccharide

IFNγ

Interferon γ

HSP60

Heat shock protein 60

Notes

Acknowledgements

This work was supported by a grant-in-aid for scientific research (C) to M.N. (17K07335) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no competing interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division of Regional Collaborative Medical Research, Office for Regional Collaboration and InnovationShimane UniversityIzumoJapan

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