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Molecular and Cellular Biochemistry

, Volume 453, Issue 1–2, pp 41–51 | Cite as

Estimating hemodynamic shear stress in murine peripheral collateral arteries by two-photon line scanning

  • Manuel Lasch
  • Katharina Nekolla
  • Anna H. Klemm
  • Judith-Irina Buchheim
  • Ulrich Pohl
  • Steffen Dietzel
  • Elisabeth DeindlEmail author
Article

Abstract

Changes in wall shear stress of blood vessels are assumed to be an important component of many physiological and pathophysiological processes. However, due to technical limitations experimental in vivo data are rarely available. Here, we investigated two-photon excitation fluorescence microscopy as an option to measure vessel diameter as well as blood flow velocities in a murine hindlimb model of arteriogenesis (collateral artery growth). Using line scanning at high frequencies, we measured the movement of blood cells along the vessel axis. We found that peak systolic blood flow velocity averaged 9 mm/s and vessel diameter 42 µm in resting collaterals. Induction of arteriogenesis by femoral artery ligation resulted in a significant increase in centerline peak systolic velocity after 1 day with an average of 51 mm/s, whereas the averaged luminal diameter of collaterals (52 µm) changed much less. Thereof calculations revealed a significant fourfold increase in hemodynamic wall shear rate. Our results indicate that two-photon line scanning is a suitable tool to estimate wall shear stress e.g., in experimental animal models, such as of arteriogenesis, which may not only help to understand the relevance of mechanical forces in vivo, but also to adjust wall shear stress in ex vivo investigations on isolated vessels as well as cell culture experiments.

Keywords

Arteriogenesis Shear stress Shear rate Two-photon line scanning 

Notes

Acknowledgements

This work was supported by the Fritz Bender Stiftung (ED) and the Friedrich-Baur-Stiftung (AK).

Compliance with ethical standards

Conflict of interest

The authors have declared they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Walter-Brendel-Centre of Experimental MedicineUniversity Hospital, LMU MunichMunichGermany
  2. 2.Core Facility Bioimaging at the Biomedical CenterLMU MunichPlanegg-MartinsriedGermany
  3. 3.Department of Anesthesiology, Laboratory for Stress and ImmunityHospital of the University of the LMU MunichMunichGermany
  4. 4.German Center for Cardiovascular ResearchPartner Site Munich Heart AllianceMunichGermany
  5. 5.Department of Otorhinolaryngology, Head & Neck SurgeryKlinikum der Universität München, Ludwig- Maximilians-Universität MünchenMunichGermany

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