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Molecular and Cellular Biochemistry

, Volume 450, Issue 1–2, pp 159–166 | Cite as

Hsa-miR-370 inhibited P-selectin-induced cell adhesion in human colon adenocarcinoma cells

  • Yunhai WeiEmail author
  • Jie Shao
  • Yan Wang
  • Hua Shen
  • Sheng Yu
  • Jinyu Zhang
  • Lei Yin
Article

Abstract

Sialyl Lewis x (sLex) is a minimal recognition motif for ligands of P-selectin and plays an important role in tumor cell adhesion and migration. Thus, targeting sLex could be an effective method to prevent tumor metastasis. In this study, we aimed to identify a microRNA (miRNA) which is capable to suppress the expression of sLex. MicroRNAs which may target ST3GAL4 were predicted by the online tools. Colo 320 HSR human colon adenocarcinoma cells were employed. The transcriptional and translational levels of ST3GAL4 were evaluated by western blotting and Real-time quantitative polymerase chain reaction. Cell adhesion and spread were assessed with or without hsa-miR-370 treatment. It was shown that hsa-miR-370 inhibited the expression of sLex in colo-320 cells, which repressed the binding of P-selectin, and led to reduced cell attachment and spread. Our results found that P-selectin-induced elevations of p-p38 and p-PI3K levels were significantly inhibited by hsa-miR-370, indicating that repressed sLex level is able to reduce the P-selectin binding and therefore eliminating the P-selectin-induced activation of p38 and PI3K signaling. In conclusion, we found that hsa-miR-370 specifically inhibits the expression of sLex, represses cell adhesion and spreading in colo-320 cells. Our study provides a possible effective treatment against tumor invasion.

Keywords

MicroRNA (miRNA) Sialyl Lewis x (sLexColon cancer P-selectin P38 

Notes

Funding

This study was supported by Social Development Project of Public Welfare Technology Research, from Science Technology Department of Zhejiang Province (2014C33137); Public Welfare Research Project, from Science Technology Department of Huzhou City (2010YSB08); Public Welfare Research Project, from Science Technology Department of Huzhou City (2017GY47).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Not applicable.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Gastrointestinal SurgeryHuzhou Central Hospital (Zhejiang University Huzhou Hospital)HuzhouChina
  2. 2.Integrated Traditional Chinese and Western Medicine SurgeryHuzhou Maternal and Child Health Care HospitalHuzhouChina

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