Insilico Studies on Antimicrobial Peptide (AMP) in Leeches

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The earthworm immune system is robust and comprises of the coelom cytolytic factor (CCF), lysenin and antimicrobial peptides (AMPs, Ghosh in Environ Sci Pollut Res 25: 6196, 2018) have been reported from organisms belonging to Phylum Annelida including earthworms (Ghosh in Int J Pept Res Ther,, 2019) which reveal structural diversity. Leeches have been known of their medical importance and known to produce AMPs (Hung et al. in J Proteomics 103:216–226, 2014; Salzet Curr Med Chem 12(6):3055–3061, 2005), but information on their detailed structures were not observed in published literature. In this study we have conducted insilico studies to understand the (i) Physicochemical properties (ii) phylogenetic relation from peptide sequence (iii) molecular modelling studies by insilico approaches which reveals overall diversity in their structure despite their similar functional role. This report highlights the properties of all AMPs in leeches with known sequences and the importance of application of insilico tools, performing a mutation analysis affecting protein stability, to highlight the significance of K(8) amino acid of the AMP 536_2 from Hirudo medicinalis in maintaining protein stability and functional importance.

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  1. Apakupakul K, Siddall ME, Burreson EM (1999) Higher level relationships of leeches (Annelida: Clitellata: Euhirudinea) based on morphology and gene sequences. Mol Phylogenet Evol 12(3):359

  2. Armenteros JJA, Tsirigos KD, Sønderby CK, Petersen TN, Winther O, Brunak S, Heijne Gv, Nielsen H (2019) SignalP 5.0 improves signal peptide predictions using deep neural networks. Nat Biotechnol 37:420–423

  3. Blom N, Gammeltoft S, Brunak S (1999) Sequence- and structure-based prediction of eukaryotic protein phosphorylation sites. J Mol Biol 294(5):1362

  4. Blom N, Sicheritz-Ponten T, Gupta R, Gammeltoft S, Brunak S (2004) Prediction of post-translational glycosylation and phosphorylation of proteins from the amino acid sequence. Proteomics 4(6):1649

  5. Ding A, Shi H, Guo Q, Liu F, Wang J, Cheng B, Wei W, Xu C (2019) Gene cloning and expression of a partial sequence of Hirudomacin, an antimicrobial protein that is increased in leech (Hirudo nipponica Whitman) after a blood meal. Comp Biochem Physiol B 231:86

  6. Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins MR, Appel RD, Bairoch A (2005) Protein identification and analysis tools on the ExPASy server. Humana Press, New York

  7. Ghosh S (2018) Environmental pollutants, pathogens and immune system in earthworms. Environ Sci Pollut Res 25:6196

  8. Ghosh S (2019) Insilico studies on antimicrobial peptides (AMPs) from earthworm. Int J Pept Res Ther.

  9. Grafskaia EN, Nadezhdin KD, Talyzina IA, Polina NF, Podgorny OV, Pavlova ER, Bashkirov PV, Kharlampieva DD, Bobrovsky PA, Latsis IA, Manuvera VA, Babenko VV, Trukhan VM, Arseniev AS, Klinov DV, Lazarev VN (2019) Medicinal leech antimicrobial peptides lacking toxicity represent a promising alternative strategy to combat antibiotic-resistant pathogens. Eur J Med Chem 180:153

  10. Hung CW, Jung S, Grötzinger J, Gelhaus C, Leippe M, Tholey A (2014) Determination of disulfide linkages in antimicrobial peptides of the macin family by combination of top-down and bottom-up proteomics. J Proteomics 103:226

  11. Jung S, Sönnichsen FD, Hung CW, Tholey A, Boidin-Wichlacz C, Haeusgen W, Gelhaus C, Desel C, Podschun R, Waetzig V, Tasiemski A, Leippe M, Grötzinger J (2012) Macin family of antimicrobial proteins combines antimicrobial and nerve repair activities. J Biol Chem 287(17):14258

  12. Kurdyumov AS, Manuvera VA, Baskova IP, Lazarev VN (2015) A comparison of the enzymatic properties of three recombinant isoforms of thrombolytic and antibacterial protein–Destabilase-Lysozyme from medicinal leech. BMC Biochem 16:27

  13. Madeira F, Park YM, Lee J, Buso N, Gur T, Madhusoodanan N, Basutkar P, Tivey ARN, Potter SC, Finn RD, Lopez R (2019) The EMBL-EBI search and sequence analysis tools APIs in 2019. Nucleic Acids Res 47(W1):W636–W641

  14. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4(4):406–425

  15. Salzet M (2005) Neuropeptide-derived antimicrobial peptides from invertebrates for biomedical applications. Curr Med Chem 12(26):61

  16. Schikorski D, Cuvillier-Hot V, Leippe M, Boidin-Wichlacz C, Slomianny C, Macagno E, Salzet M, Tasiemski A (2008) Microbial challenge promotes the regenerative process of the injured central nervous system of the medicinal leech by inducing the synthesis of antimicrobial peptides in neurons and microglia. J Immunol 181(2):1083–1095

  17. Tasiemski A, Verger-Bocquet M, Cadet M, Goumon Y, Metz-Boutigue MH, Aunis D, Stefano GB, Salzet M (2000) Proenkephalin A-derived peptides in invertebrate innate immune processes. Brain Res Mol Brain Res 76(2):237–252

  18. Tasiemski A, Vandenbulcke F, Mitta G, Lemoine J, Lefebvre C, Sautière PE, Salzet M (2004) Molecular characterization of two novel antibacterial peptides inducible upon bacterial challenge in an annelid, the leech Theromyzon tessulatum. J Biol Chem 279(30):30982

  19. Tasiemski A, Massol F, Cuvillier-Hot V, Boidin-Wichlacz C, Roger E, Rodet F, Fournier I, Thomas F, Salzet M (2015) Reciprocal immune benefit based on complementary production of antibiotics by the leech Hirudo verbana and its gut symbiont Aeromonas veronii. Sci Rep 5:17498

  20. Tasiemski A, Salzet M (2017) Neuro-immune lessons from an annelid: The medicinal leech. Dev Comp Immunol 66:33–42

  21. Xu D, Zhang Y (2012) Ab initio protein structure assembly using continuous structure fragments and optimized knowledge-based force field. Proteins 80:1735

  22. Xu D, Zhang Y (2013) Toward optimal fragment generations for ab initio protein structure assembly. Proteins 81:239

  23. Yu CS, Lin CJ, Hwang JK (2004) Predicting subcellular localization of proteins for Gram-negative bacteria by support vector machines based on n-peptide compositions. Protein Sci 13:1402–1406

  24. Yu CS, Chen YC, Lu CH, Hwang JK (2006) Prediction of protein subcellular localization. Proteins 64:651

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The author acknowledges NISER Bhubaneswar for the study.

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Correspondence to Shyamasree Ghosh.

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Not applicable as it is an insilico study

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Not applicable since it is an insilico study.

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Ghosh, S. Insilico Studies on Antimicrobial Peptide (AMP) in Leeches. Int J Pept Res Ther (2020) doi:10.1007/s10989-020-10017-7

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  • Leech
  • Antimicrobial peptide (AMP)
  • Molecular modelling