Vaspin Exert Anti-Inflammatory and Antioxidant Effects on Renal and Liver Injury Induced by Renal Ischemia Reperfusion
- 5 Downloads
Renal ischemia–reperfusion (IR) is a common cause of acute renal failure and result in remote organ injury. Oxidative stress and inflammation are of the suggested causes of renal IR injury. The aim of the present study was to investigate the protective effects of vaspin as an adipokine on renal and liver injury after renal IR. 36 Male mice randomly assigned to four groups: (1) Sham operated (2) renal IR (45 min bilateral ischemia) (3 & 4) renal IR + vaspin (20 & 40 mg/kg). Animals were intragastrically administered with PBS (Sham) or vaspin (20 & 40 mg/kg) for 7 days before renal IR. Renal IR induced renal functional damage, oxidative stress and inflammation in kidney and liver. Treatment with vaspin decreased plasma BUN, creatinine, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) and increased creatinine clearance and urine flow rate. In both renal and liver tissues a significant rise in superoxide dismutase, catalase, interleukin-10 and fall in Malondialdehyde and tumor necrosis factor-α were seen after treatment. Oral administration of vaspin diminished renal and hepatic functional injury, oxidative stress and inflammation.
KeywordsRenal ischemia–reperfusion Vaspin Adipokine Oxidative stress Inflammation Remote organ injury
Compliance with Ethical Standards
Conflict of interest
The authors declared that they have no conflict of interest.
Informed consent was obtained from all individual participants included in the study.
- Chi L, Hu X, Zhang W, Bai T, Zhang L, Zeng H, Guo R, Zhang Y, Tian H (2017) Adipokine CTRP6 improves PPARγ activation to alleviate angiotensin II-induced hypertension and vascular endothelial dysfunction in spontaneously hypertensive rats. Biochem Biophys Res Commun 482:727–734CrossRefGoogle Scholar
- Esterbauer H, Cheeseman KH (1990)  determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods in enzymology, Elsevier, AmsterdamGoogle Scholar
- Hadj Abdallah N, Baulies A, Bouhlel A, Bejaoui M, Zaouali MA, Ben Mimouna S, Messaoudi I, Fernandez-Checa JC, García Ruiz C, Ben Abdennebi H (2018) Zinc mitigates renal ischemia-reperfusion injury in rats by modulating oxidative stress, endoplasmic reticulum stress, and autophagy. J Cell Physiol 233(11):8677–8690CrossRefGoogle Scholar
- Kadkhodaee M, Khastar H, Seifi B, Najafi A, Delavari F (2012) Recipient kidney damage after leukocyte transfer from inbred mice with renal ischemia-reperfusion injury. Tehran Univ Med Sci 70(2):69–77Google Scholar
- Li W, Qi D, Chen L, Zhao Y, Deng W, Tang X, Wang D (2018) Vaspin protects against lipopolysaccharide-induced acute respiratory distress syndrome in mice by inhibiting inflammation and protecting vascular endothelium via PI3K/Akt signal pathway. Nan fang yi ke da xue xue bao = Journal of Southern Medical University 38:283–288.PubMedGoogle Scholar
- Mohamed NSE-D, Mubarak H (2011) Effects of renal ischemia reperfusion on brain, liver kidney tissues in adult male rats. Life Sci J 8:204–212Google Scholar
- Phalitakul S, Okada M, Hara Y, Yamawaki H (2013) Vaspin prevents methylglyoxal-induced apoptosis in human vascular endothelial cells by inhibiting reactive oxygen species generation. Acta Physiol 209:212–219Google Scholar
- Qi D, Wang D, Zhang C, Tang X, He J, Zhao Y, Deng W, Deng X (2017) Vaspin protects against LPS-induced ARDS by inhibiting inflammation, apoptosis and reactive oxygen species generation in pulmonary endothelial cells via the Akt/GSK-3β pathway. Int J Mol Med 40:1803–1817PubMedPubMedCentralGoogle Scholar
- Rodríguez A, Ezquerro S, Méndez-Giménez L, Becerril S, Frühbeck G (2015) Revisiting the adipocyte: a model for integration of cytokine signaling in the regulation of energy metabolism. Am J Physiol 309(8):E691–E714Google Scholar