HESA-A Attenuates Hepatic Steatosis in NAFLD Rat Model Through the Suppression of SREBP-1c and NF-kβ

  • M. Efati
  • M. Khorrami
  • Z. JangraviEmail author
  • A. Z. MahmoudabadiEmail author
  • M. Raeiszadeh
  • J. R. Sarshoori


Nonalcoholic fatty liver disease (NAFLD) is a chronic disorder, which is mainly considered a result of high-fat diet in humans. The clinical spectrum of NAFLD ranges from simple steatosis to nonalcoholic steatohepatitis (NASH) with irreversible complications. Therefore, early treatments, especially with medicinal plants can be considered as an important strategy in NAFLD treatment. This study aimed at investigating the effect of HESA-A (a herbal-marine supplement) on treatment of fatty liver in rats by evaluation of SREBP-1c and NF-kβ genes expression and biochemical analyses. In an experimental study, 28 male Wistar rats (weighing 180 ± 20 g), were divided to two groups and treated with a standard diet (n = 7) and a high-fat diet (HFD) (n = 21) for eight weeks. In order to confirm the NAFLD, the control group and seven rats of the HFD group were killed and biochemical parameters and histopathological changes were analyzed. The rest of the rats were divided to two groups and fed either atorvastatin (30 mg/kg/day) or HESA-A (500 mg/kg/day) for 30 days. Finally, serum biomarkers of liver damage, serum lipid profiles and gene expression of NF-kβ and SREBP-1c were investigated. Semi-quantitative Real Time-Polymerase Chain Reaction (RT-PCR) analyses showed that the expression of SREBP-1c and NF-kβ increased in NAFLD rat model compared to the control group. The expression level of SREBP-1c and NF-kβ genes showed a significant decrease in HESA-A and atorvastatin-treated groups in comparison to the control group. Biochemical analyses also confirmed these results. These findings showed that HESA-A could be used as an effective alternative in treatment of NAFLD with low side effects in comparison with atorvastatin as current medication.


NAFLD Hepatic steatosis HESA-A NF-kβ SREBP-1c 



We would like to thank BMSU for allowing the use of the research laboratory.


This work was supported by Department of Biochemistry of BMSU and the authors.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no competing interests.


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© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Biochemical Department of Medical FacultyBaqiyatallah University of Medical ScienceTehranIran
  2. 2.Nanobiotechnology Research CenterBaqiyatallah University of Medical ScienceTehranIran
  3. 3.Department of SurgeryBaqiyatallah University of Medical SciencesTehranIran
  4. 4.Anatomy Department of Medical FacultyBaqiyatallah University of Medical ScienceTehranIran

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