Identification of an Immunoglobulin M (IgM) Antibody Against Enolase 1 Protein (ENO1) Derived from HEK-293 Cells in Patients with Kidney Failure
- 4 Downloads
Kidney failure is one of the most important challenges in medicine. In this study, we used HEK-293 kidney cells to evaluate and compare changes in the expression of natural antibodies in patients with kidney failure and healthy controls. We analyzed the immunoreactivity of two groups of pooled sera from patient and control groups against antigens derived from HEK-293 cell lysates. The cell lysates were immunoblotted with the pooled sera from the groups. The results showed that the patients’ sera contained IgM antibodies, which recognized enolase 1 proteins.
KeywordsKidney failure HEK-293 cell line Enolase 1 Two-dimensional electrophoresis
MHJ, MS: designed research; AM: performed research; AM, RN: analyzed data; MHJ, RN: wrote the manuscript; MHJ: performed statistical analysis.
This study was financially supported by a research Grant from the Iran National Science Foundation.
Compliance with Ethical Standards
Conflict of interest
The authors have declared no conflict of interest.
All procedures were performed in accordance with the ethical standards of the local ethics committee of Iran University of Medical Sciences and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards (Grant No: 88001856).
All authors are aware of and agree to the content of the manuscript.
- Bussone G, Dib H, Tamby MC, Broussard C, Federici C, Woimant G, Camoin L, Guillevin L, Mouthon L (2011) Identification of new autoantibody specificities directed at proteins involved in the transforming growth factor b pathway in patients with systemic sclerosis. Arthritis Res Ther 13:74CrossRefGoogle Scholar
- Devarajan P (2007) Proteomics for biomarker discovery in acute kidney injury. Seminars in nephrology. Elsevier, AmsterdamGoogle Scholar
- Guilpain P, Servettaz A, Tamby MC, Chanseaud Y, Tamas N, Pena-Lefebvre PGDL, Broussard C, Guillevin L, Camoin L, Mouthon L (2007) A combined SDS-PAGE and proteomics approach to identify target autoantigens in healthy individuals and patients with autoimmune diseases. Ann N Y Acad Sci 1109(1):538–549CrossRefGoogle Scholar
- Lefranc D, Launay D, Dubucquoi S, Seze JD, Dussart P, Vermersch M, Hachulla E, Hatron PY, Vermersch P, Mouthon L (2007) Characterization of discriminant human brain antigenic targets in neuropsychiatric systemic lupus erythematosus using an immunoproteomic approach. Arthritis Rheum 56(10):3420–3432CrossRefGoogle Scholar
- Mostafaie A, Yari K, Kiani S (2013) A comparative evaluation of rehydration and cuploading sample application for modified twodimensional gel electrophoresis of human serum proteins using immobilized pH gradient. Afr J Biotechnol 10(55):11711–11715Google Scholar
- Murphy R, Sites J, Buth D, Haufler CH (1990) Proteins I: isozyme electrophoresis. In: Hillis DM, Moritz CM (eds) Molecular systematics. Sunderland, Sinauer, pp 45–126Google Scholar
- Remy A (2002) Focusing strategy and influence of conductivity on isoelectric focusing in immobilized pH gradients. http://www.semanticscholar.org/paper/Focusing-Strategy-and-Influence-of-Conductivity-on-Remy/22f9bae4a779f0f71128fdd95205fea427beba52.
- Snedecor GW, Cochran WG (1989) Statistical Methods, eight edition. Iowa state University Press, AmesGoogle Scholar
- Srinivas PR, Srivastava S, Hanash S, Wright GL (2001) Proteomics in early detection of cancer. Clin Chem 47(10):1901–1911Google Scholar