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Identification of an Immunoglobulin M (IgM) Antibody Against Enolase 1 Protein (ENO1) Derived from HEK-293 Cells in Patients with Kidney Failure

  • Mir Hadi JazayeriEmail author
  • Maryam Sadri
  • Ali Mostafaie
  • Reza Nedaeinia
Article
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Abstract

Kidney failure is one of the most important challenges in medicine. In this study, we used HEK-293 kidney cells to evaluate and compare changes in the expression of natural antibodies in patients with kidney failure and healthy controls. We analyzed the immunoreactivity of two groups of pooled sera from patient and control groups against antigens derived from HEK-293 cell lysates. The cell lysates were immunoblotted with the pooled sera from the groups. The results showed that the patients’ sera contained IgM antibodies, which recognized enolase 1 proteins.

Graphic Abstract

Keywords

Kidney failure HEK-293 cell line Enolase 1 Two-dimensional electrophoresis 

Notes

Author Contributions

MHJ, MS: designed research; AM: performed research; AM, RN: analyzed data; MHJ, RN: wrote the manuscript; MHJ: performed statistical analysis.

Funding

This study was financially supported by a research Grant from the Iran National Science Foundation.

Compliance with Ethical Standards

Conflict of interest

The authors have declared no conflict of interest.

Ethical Approval

All procedures were performed in accordance with the ethical standards of the local ethics committee of Iran University of Medical Sciences and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards (Grant No: 88001856).

Informed Consent

All authors are aware of and agree to the content of the manuscript.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Mir Hadi Jazayeri
    • 1
    • 2
    Email author
  • Maryam Sadri
    • 2
  • Ali Mostafaie
    • 3
  • Reza Nedaeinia
    • 4
  1. 1.Immunology Research CenterIran University of Medical SciencesTehranIran
  2. 2.Department of Immunology, Faculty of Medical SciencesIran University of Medical SciencesTehranIran
  3. 3.Medical Biology Research CenterKermanshah University of Medical SciencesKermanshahIran
  4. 4.Applied Physiology Research Center, Cardiovascular Research InstituteIsfahan University of Medical ScienceIsfahanIran

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