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Survivin as a Target for Anti-cancer Phytochemicals According to the Molecular Docking Analysis

  • Kobra Foroughi
  • Sarvin Jahanbani
  • Simin Nazarnezhad
  • Hossein Khastar
  • Moslem JafarisaniEmail author
  • Mersedeh TashakoriEmail author
  • Seyedeh Sareh Kazemi
Article
  • 8 Downloads

Abstract

Survivin is a unique member of the inhibitor of apoptosis protein family. Research has approved Survivin’s ability to interact with Smac/DIABLO, suggesting that Survivin may suppress activation of caspases indirectly. On the other hand, research has demonstrated that many drugs for cancer therapy are unsuccessful and disappointing in advanced stages. Therefore, it is necessary to find new drugs with the highest anti-cancer properties and lowest side effects. We investigated the interaction of some phytochemicals with BIR domain of Survivin. In this study, the 3D structures of some phytochemicals, including Berberine, Carvacrol, Crocetin, Crocin, Curcumin, Picrocrocin, Piperine, and Thymol, were harnessed from Human Metabolome Database, which has reported some evidence about these phytochemicals’ anti-cancer effect via the apoptosis induction with Survivin. Then, these structures were prepared for molecular docking analysis by Autodock Vina software. Ultimately, the binding energies between docked Survivin and the above mentioned phytochemicals were calculated and their interactions were predicted. Our results indicated that all phytochemicals can interact with Survivin molecule in active site of Smac/DIABLO and the best minimum binding energies belong to Piperine and Picrocrocin. It is concluded that, out of the studied compounds, Piperine and Picrocrocin could act as potential inhibitors of Survivin.

Graphic Abstract

Keywords

Survivin Smac/DIABLO Inhibitors Autodock Vina Phytochemicals Berberine Piperine 

Notes

Acknowledgements

This study was supported by Grant No 9775 from Shahroud University of Medical Sciences.

Funding

This study was funded by Shahroud University of Medical Sciences (Grant No 9775).

Compliance with Ethical Standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors. However, we have ethical cod approve IR.SHMU.REC.1397.112 by ethical committee of SHMU.

References

  1. Abrams SL et al (2019) Abilities of berberine and chemically modified berberines to inhibit proliferation of pancreatic cancer cells. Adv Biol Regul 71:172–182.  https://doi.org/10.1016/j.jbior.2018.10.003 CrossRefGoogle Scholar
  2. Amerizadeh F et al (2018) Crocin synergistically enhances the antiproliferative activity of 5-flurouracil through Wnt/PI3K pathway in a mouse model of colitis-associated colorectal cancer. J Cell Biochem 119:10250–10261CrossRefGoogle Scholar
  3. Arzi L, Farahi A, Jafarzadeh N, Riazi G, Sadeghizadeh M, Hoshyar R (2018) Inhibitory effect of crocin on metastasis of triple-negative breast cancer by interfering with Wnt/β-catenin pathway in murine model. DNA Cell Biol 37:1068–1075CrossRefGoogle Scholar
  4. Bakshi HA, Hakkim FL, Sam S (2016) Molecular mechanism of crocin induced caspase mediated MCF-7 cell death: in vivo toxicity profiling and ex vivo macrophage activation. Asian Pac J Cancer Prev 17:1499–1506CrossRefGoogle Scholar
  5. Bathaie SZ, Mousavi SZ (2010) New applications and mechanisms of action of saffron and its important ingredients. Crit Rev Food Sci Nutr 50:761–786.  https://doi.org/10.1080/10408390902773003 CrossRefGoogle Scholar
  6. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A (2018) Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 68:394–424.  https://doi.org/10.3322/caac.21492 CrossRefGoogle Scholar
  7. Chen S et al (2015) Crocin inhibits cell proliferation and enhances cisplatin and pemetrexed chemosensitivity in lung cancer cells. Transl Lung Can Res 4:775–783.  https://doi.org/10.3978/j.issn.2218-6751.2015.11.03 Google Scholar
  8. Chidambara Murthy KN, Jayaprakasha GK, Patil BS (2012) The natural alkaloid berberine targets multiple pathways to induce cell death in cultured human colon cancer cells. Eur J Pharmacol 688:14–21.  https://doi.org/10.1016/j.ejphar.2012.05.004 CrossRefGoogle Scholar
  9. Dai W, Sun C, Huang S, Zhou Q (2016) Carvacrol suppresses proliferation and invasion in human oral squamous cell carcinoma. OncoTargets Ther 9:2297–2304.  https://doi.org/10.2147/OTT.S98875 CrossRefGoogle Scholar
  10. Dai W, Mu L, Cui Y, Li Y, Chen P, Xie H, Wang X (2019) Berberine promotes apoptosis of colorectal cancer via regulation of the long non-coding RNA (lncRNA) cancer susceptibility candidate 2 (CASC2)/AU-binding factor 1 (AUF1)/B-Cell CLL/lymphoma 2 (Bcl-2) axis. Med Sci Monit 25:730–738.  https://doi.org/10.12659/msm.912082 CrossRefGoogle Scholar
  11. Du J, Kelly AE, Funabiki H, Patel DJ (2012) STRUCTURAL basis for recognition of H3T3ph and Smac/DIABLO N-terminal peptides by human. Surviv Struct 20:185–195.  https://doi.org/10.1016/j.str.2011.12.001 CrossRefGoogle Scholar
  12. Fan K, Li X, Cao Y, Qi H, Li L, Zhang Q, Sun H (2015) Carvacrol inhibits proliferation and induces apoptosis in human colon cancer cells. Anticancer Drugs 26:813–823.  https://doi.org/10.1097/cad.0000000000000263 CrossRefGoogle Scholar
  13. Faridi N, Heidarzadeh H, Mohagheghi MA, Bathaie SZ (2019) BT-474 breast cancer cell apoptosis induced by crocin, a saffron carotenoid. Basic Clin Cancer Res 11:5–15Google Scholar
  14. Festuccia C, Colapietro A, Mancini A, D’alessandro A (2018) Crocetin and crocin from saffron in cancer chemotherapy and chemoprevention. Anti Cancer Agents Med Chem 19:38–47Google Scholar
  15. Gan R-Y (2012) Bioactivities of berberine: an Update, vol 1Google Scholar
  16. Global Burden of Disease Cancer C (2017) Global, regional, and national cancer incidence, mortality, years of life lost, years lived with disability, and disability-adjusted life-years for 32 cancer groups, 1990 to 2015: a systematic analysis for the global burden of disease study JAMA. Oncology 3:524–548.  https://doi.org/10.1001/jamaoncol.2016.5688 Google Scholar
  17. Groner B, Weiss A (2014) Targeting survivin in cancer: novel drug development approaches. Biodrugs 28:27–39.  https://doi.org/10.1007/s40259-013-0058-x CrossRefGoogle Scholar
  18. Gutheil WG, Reed G, Ray A, Dhar A (2012) Crocetin: an agent derived from saffron for prevention and therapy for cancer. Curr Pharm Biotechnol 13:173–179CrossRefGoogle Scholar
  19. Hassan M, Watari H, AbuAlmaaty A, Ohba Y, Sakuragi N (2014) Apoptosis and molecular targeting therapy in cancer. Biomed Res Int 2014:150845.  https://doi.org/10.1155/2014/150845 Google Scholar
  20. Hassanalilou T, Ghavamzadeh S, Khalili L (2019) Curcumin and gastric cancer: a review on mechanisms of action. J Gastrointest Cancer 15:185–192.  https://doi.org/10.1007/s12029-018-00186-6 CrossRefGoogle Scholar
  21. Heidarzadeh H, Bathaie SZ, Abroun S, Mohagheghi MA (2018) Evaluating the cytotoxic effect of crocin on MDA-MB-468 cell line based on apoptosis induction, ER stress, and autophagy markers. Pathol Res 20:37–51Google Scholar
  22. Jabbarzadeh Kaboli P, Rahmat A, Ismail P, Ling K-H (2014) Targets and mechanisms of berberine, a natural drug with potential to treat cancer with special focus on breast cancer. Eur J Pharmacol 740:584–595.  https://doi.org/10.1016/j.ejphar.2014.06.025 CrossRefGoogle Scholar
  23. Jafarisani M, Bathaie SZ, Mousavi MF (2018) Saffron carotenoids (crocin and crocetin) binding to human serum albumin as investigated by different spectroscopic methods and molecular docking. J Biomol Struct Dyn 36:1681–1690CrossRefGoogle Scholar
  24. Jafri A et al (2019) Induction of apoptosis by piperine in human cervical adenocarcinoma via ROS mediated mitochondrial pathway and caspase-3 activation. EXCLI J 18:154–164.  https://doi.org/10.17179/excli2018-1928
  25. Jaiswal PK, Goel A, Mittal RD (2015) Survivin: a molecular biomarker in cancer. Indian J Med Res 141:389–397.  https://doi.org/10.4103/0971-5916.159250 CrossRefGoogle Scholar
  26. Kang SH et al (2016a) Anticancer effect of thymol on ags human gastric carcinoma cells. J Microbiol Biotechnol 26:28–37.  https://doi.org/10.4014/jmb.1506.06073 CrossRefGoogle Scholar
  27. Kang Y et al (2016b) Curcumin sensitizes human gastric cancer cells to 5-fluorouracil through inhibition of the NFκB survival-signaling pathway. OncoTargets Ther 9:7373–7384.  https://doi.org/10.2147/ott.s118272 CrossRefGoogle Scholar
  28. Kapellos G et al (2013) Overexpression of survivin levels in circulation and tissue samples of lung cancer patients. Anticancer Res 33:3475–3480Google Scholar
  29. Khalili S, Zakeri A, Hashemi Zahra S, Masoumikarimi M, Rezaei Manesh Mohammad R, Shariatifar N, Jafari Sani M (2017) Structural analyses of the interactions between the thyme active ingredients and human serum albumin. Turk J Biochem 42:459–467.  https://doi.org/10.1515/tjb-2017-0008 Google Scholar
  30. Khan Z, Khan AA, Yadav H, Prasad GBKS, Bisen PS (2017) Survivin, a molecular target for therapeutic interventions in squamous cell carcinoma. Cell Mol Biol Lett 22:8.  https://doi.org/10.1186/s11658-017-0038-0 CrossRefGoogle Scholar
  31. Khorasanchi Z et al (2018) Crocus sativus a natural food coloring and flavoring has potent anti-tumor properties. Phytomedicine 43:21–27.  https://doi.org/10.1016/j.phymed.2018.03.041 CrossRefGoogle Scholar
  32. Kim JY et al (2006) Nuclear interaction of Smac/DIABLO with Survivin at G2/M arrest prompts docetaxel-induced apoptosis in DU145 prostate cancer cells. Biochem Biophys Res Commun 350:949–954.  https://doi.org/10.1016/j.bbrc.2006.09.143 CrossRefGoogle Scholar
  33. Kunihiro AG, Brickey JA, Frye JB, Luis PB, Schneider C, Funk JL (2019) Curcumin, but not curcumin-glucuronide, inhibits Smad signaling in TGFβ-dependent bone metastatic breast cancer cells and is enriched in bone compared to other tissues. J Nutr Biochem 63:150–156CrossRefGoogle Scholar
  34. Lahazi V, Taheri G, Jafarisani M (2015) Antioxidant enzymes activity of Ferula flabelliloba and Ferula diversivitata extracts Turkish. J Biochem 40:310–315.  https://doi.org/10.1515/tjb-2015-0016 Google Scholar
  35. Li S et al (2015) Anticancer effects of crocetin in human esophageal squamous cell carcinoma KYSE-150 cells. Oncol Lett 9:1254–1260.  https://doi.org/10.3892/ol.2015.2869 CrossRefGoogle Scholar
  36. Li Y et al (2012) Taurine attenuates methamphetamine-induced autophagy and apoptosis in PC12 cells through mTOR signaling pathway. Toxicol Lett 215:1–7.  https://doi.org/10.1016/j.toxlet.2012.09.019 CrossRefGoogle Scholar
  37. Lim W, Ham J, Bazer FW, Song G (2019) Carvacrol induces mitochondria-mediated apoptosis via disruption of calcium homeostasis in human choriocarcinoma cells. J Cell Physiol 234:1803–1815CrossRefGoogle Scholar
  38. Lu B, Hu M, liu K, Peng J (2010) Cytotoxicity of berberine on human cervical carcinoma HeLa cells through mitochondria, death receptor and MAPK pathways, and in silico drug-target prediction. Toxicol In Vitro 24:1482–1490.  https://doi.org/10.1016/j.tiv.2010.07.017 CrossRefGoogle Scholar
  39. Lu P, Lin H, Gu Y, Li L, Guo H, Wang F, Qiu X (2015) Antitumor effects of crocin on human breast cancer cells. Int J Clin Exp Med 8:20316–20322Google Scholar
  40. Mahmudabadi AZ, Karimi MM, Bahabadi M, Hoseinabadi ZB, JafariSani M, Ahmadi R (2016) Inhibition of AGS cancer cell proliferation following siRNA-mediated downregulation of VEGFR2. Cell J 18:381–388Google Scholar
  41. Manayi A, Nabavi SM, Setzer WN, Jafari S (2017) Piperine as a potential anti-cancer agent: a review on preclinical studies. Curr Med Chem 1:1.  https://doi.org/10.2174/0929867324666170523120656 Google Scholar
  42. McCubrey JA et al. (2017) Effects of resveratrol, curcumin, berberine and other nutraceuticals on aging, cancer development, cancer stem cells and microRNAs. Aging 9:1477–1536.  https://doi.org/10.18632/aging.101250
  43. McNeish IA et al (2005) Survivin interacts with Smac/DIABLO in ovarian carcinoma cells but is redundant in Smac-mediated apoptosis. Exp Cell Res 302:69–82.  https://doi.org/10.1016/j.yexcr.2004.08.029 CrossRefGoogle Scholar
  44. Meng X-Y, Zhang H-X, Mezei M, Cui M (2011) Molecular docking: a powerful approach for structure-based drug discovery. Curr Comput Aided Drug Des 7:146–157CrossRefGoogle Scholar
  45. Milajerdi A, Haghighatdoost F, Azadbakht L (2015) Saffron (Crocus satious L) and its crocin and crocetin toxicity against normal and tumor cells: a systematic review. Review.  https://doi.org/10.1016/j.jnim.2015.12.332 Google Scholar
  46. Miller Kimberly D et al (2016) Cancer treatment and survivorship statistics, 2016. CA Cancer J Clin 66:271–289.  https://doi.org/10.3322/caac.21349 CrossRefGoogle Scholar
  47. Mobahat M, Narendran A, Riabowol K (2014) Survivin as a preferential target for cancer therapy. Int J Mol Sci 15:2494–2516.  https://doi.org/10.3390/ijms15022494 CrossRefGoogle Scholar
  48. Moosavi MA, Rahmati M, Ashtari N, Alizadeh J, Hashemi M, Bathaei SZ, Ghavami S (2017) Apoptosis, autophagy, and unfolded protein response and cerebellar development. Development of the cerebellum from molecular aspects to diseases. Springer, New York, pp 153–178CrossRefGoogle Scholar
  49. Moradzadeh M, Sadeghnia HR, Tabarraei A, Sahebkar A (2018) Anti-tumor effects of crocetin and related molecular targets. J Cell Physiol 233:2170–2182.  https://doi.org/10.1002/jcp.25953 CrossRefGoogle Scholar
  50. Nagoor Meeran MF, Javed H, Al Taee H, Azimullah S, Ojha SK (2017) Pharmacological Properties and molecular mechanisms of thymol: prospects for its therapeutic potential and pharmaceutical development. Front Pharmacol 8:380.  https://doi.org/10.3389/fphar.2017.00380 CrossRefGoogle Scholar
  51. Park W, Amin ARMR, Chen ZG, Shin DM (2013) New perspectives of curcumin in cancer prevention. Cancer Prev Res 6:387–400.  https://doi.org/10.1158/1940-6207.capr-12-0410 CrossRefGoogle Scholar
  52. Patel SS, Acharya A, Ray RS, Agrawal R, Raghuwanshi R, Jain P (2019) Cellular and molecular mechanisms of curcumin in prevention and treatment of disease. Critical Rev Food Sci Nutr.  https://doi.org/10.1080/10408398.2018.1552244 Google Scholar
  53. Pavlyukov MS, Antipova NV, Balashova MV, Vinogradova TV, Kopantzev EP, Shakhparonov MI (2011) Survivin monomer plays an essential role in apoptosis regulation. J Biol Chem 286:23296–23307.  https://doi.org/10.1074/jbc.M111.237586 CrossRefGoogle Scholar
  54. Potočnjak I, Gobin I, Domitrović R (2018) Carvacrol induces cytotoxicity in human cervical cancer cells but causes cisplatin resistance: involvement of MEK–ERK activation. Phytother Res 32:1090–1097CrossRefGoogle Scholar
  55. Rather RA, Bhagat M (2018) Cancer chemoprevention and piperine: molecular mechanisms and therapeutic opportunities. Front Cell Dev Biol 6:10.  https://doi.org/10.3389/fcell.2018.00010 CrossRefGoogle Scholar
  56. Ravindran J, Prasad S, Aggarwal BB (2009) Curcumin and cancer cells: how many ways can curry kill tumor cells selectively? AAPS J 11:495–510.  https://doi.org/10.1208/s12248-009-9128-x CrossRefGoogle Scholar
  57. Rothwell DG, Barzilay G, German M, Morera S, Freemont P, Hickson LD (1997) The structure and functions of the HAPl/Ref-1 protein. Oncol Res 9:275–280Google Scholar
  58. Sah NK, Khan Z, Khan GJ, Bisen PS (2006) Structural, functional and therapeutic biology of survivin. Cancer Lett 244:164–171.  https://doi.org/10.1016/j.canlet.2006.03.007 CrossRefGoogle Scholar
  59. Sani MJ, Yazdi F, Karimi MM, Alizadeh J, Rahmati M, Mahmudabadi AZ (2016) The siRNA-mediated down-regulation of vascular endothelial growth factor receptor1. Iran Red Crescent Med J 18:381–388Google Scholar
  60. Shariatifar N, Shoeibi S, Sani MJ, Jamshidi AH, Zarei A, Mehdizade A, Dadgarnejad M (2014) Study on diuretic activity of saffron (stigma of Crocus sativus L.) Aqueous extract in rat. J Adv Pharm Technol Res 5:17CrossRefGoogle Scholar
  61. Song Z, Yao X, Wu M (2003) Direct interaction between survivin and Smac/DIABLO is essential for the anti-apoptotic activity of survivin during taxol-induced apoptosis. J Biol Chem 278:23130–23140.  https://doi.org/10.1074/jbc.M300957200 CrossRefGoogle Scholar
  62. Sriwiriyajan S, Tedasen A, Lailerd N, Boonyaphiphat P, Nitiruangjarat A, Deng Y, Graidist P (2016) Anticancer and Cancer prevention effects of piperine-free Piper nigrum extract on N-nitrosomethylurea-induced mammary tumorigenesis in rats. Cancer Prev Res 9:74.  https://doi.org/10.1158/1940-6207.capr-15-0127 CrossRefGoogle Scholar
  63. Suk Choi M et al (2009) Berberine inhibits p53-dependent cell growth through induction of apoptosis of prostate cancer cells. Int J Oncol 34:1221–1230.  https://doi.org/10.3892/ijo_00000250 Google Scholar
  64. Sun C, Nettesheim D, Liu Z, Olejniczak ET (2005) Solution structure of human survivin and its binding interface with Smac/Diablo. Biochemistry 44:11–17.  https://doi.org/10.1021/bi0485171 CrossRefGoogle Scholar
  65. Trott O, Olson AJ (2010) AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization and multithreading. J Comput Chem 31:455–461.  https://doi.org/10.1002/jcc.21334 Google Scholar
  66. Wishart DS et al (2007) HMDB: the human metabolome database. Nucleic Acids Res 35:D521–D526.  https://doi.org/10.1093/nar/gkl923 CrossRefGoogle Scholar
  67. Xu J et al (2019) Anticancer effect of berberine based on experimental animal models of various cancers: a systematic review and meta-analysis. BMC Cancer 19:589–589.  https://doi.org/10.1186/s12885-019-5791-1 CrossRefGoogle Scholar
  68. Yaffe PB, Power Coombs M, Doucette C, Walsh M, Hoskin D (2014) Piperine, an alkaloid from black pepper, inhibits growth of human colon cancer cells via G1 arrest and apoptosis triggered by endoplasmic reticulum stress. Mol Carcinogen 54:1070–1085.  https://doi.org/10.1002/mc.22176 CrossRefGoogle Scholar
  69. Yu M et al (2013) Berberine enhances chemosensitivity to irinotecan in colon cancer via inhibition of NF-κB. Mol Med Rep 9:249–254.  https://doi.org/10.3892/mmr.2013.1762 CrossRefGoogle Scholar
  70. Yu L, Li J, Xiao M (2018) Picrocrocin exhibits growth inhibitory effects against SKMEL- 2 human malignant melanoma cells by targeting JAK/STAT5 signaling pathway, cell cycle arrest and mitochondrial mediated apoptosis. J BUON 23:1163–1168Google Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Student Research Committee, School of MedicineShahroud University of Medical SciencesShahroudIran
  2. 2.Cancer Prevention Research CenterShahroud University of Medical SciencesShahroudIran
  3. 3.Department of BiologyIslamic Azad University, Science and Research BranchTehranIran
  4. 4.Department of BiochemistryDamghan Branch of Islamic Azad UniversityDamghanIran

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