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Intensifying the Anticancer Potential of Cationic Peptide Derived from Serine Threonine Protein Kinase of Teleost by Tagging with Oligo Tryptophan

  • Nagaram Prabha
  • Anbazahan Sannasimuthu
  • Venkatesh Kumaresan
  • Preetham Elumalai
  • Jesu ArockiarajEmail author
Article
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Abstract

Serine threonine protein kinase plays an important role in the cell growth, cell cycle regulation and development which shows a significant role in signal transduction pathway. In this study, a cationic peptide IE13 was derived from serine threonine protein kinase of a teleost fish and it was modified as IW13 by replacing the C-terminal residue with tryptophan. The anticancer potential of IE13 and IW13 was tested against three different types of cell lines HeLa (Human Cervical carcinoma cells), A549 (Human lung cancer cells) and MCF 7 (Human breast cancer cells) at different concentrations (300, 100, 30 and 10 µM) in semi logarithmic range. The results showed that the peptide IW13 showed EC50 of 92 µM for MCF 7, 102 µM for A549 and 85 µM for HeLa whereas, IE13 showed less growth inhibition on the tested cell lines. The flow cytometry study was conducted to demonstrate the cell cycle inhibition by the cationic peptide IW13 against HeLa cell line. Cytotoxicity studies of the cationic peptide showed no cytotoxicity against normal cell line (RAW 264.7). Tailoring of these properties was likely to be a key in safe and successful transfer of this peptide from laboratory experiments into clinical practice for pharmaceutical formulations.

Keywords

Serine threonine protein kinase Cationic antimicrobial peptide Cytotoxicity Anticancer HeLa A549 MCF 7 

Abbreviations

STPK

Serine threonine protein kinase

HeLa

Human cervical carcinoma cells

A549

Adenocarcinoma human alveolar basal epithelial cells

MCF 7

Human breast cancer cells (Michigan Cancer Foundation 7)

CAPs

Cationic antimicrobial peptides

PS

Phosphatidyl serine

Trp

Tryptophan

Cs

Channa striatus

RAW 264.7

Murine macrophage cell line

DMEM

Dulbecco’s modified eagle’s medium

FCS

Foetal calf serum

GIBCO

GIBCO cell culture media and reagents

OD

Optical density

PBS

Phosphate buffered saline

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Notes

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research Involving Human and Animal Participants

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of MicrobiologySRM Arts & Science CollegeChennaiIndia
  2. 2.Department of Biotechnology, Faculty of Science and HumanitiesSRM Institute of Science and TechnologyChennaiIndia
  3. 3.Department of Processing Technology (Biochemistry)Kerala University of Fisheries and Ocean StudiesKochiIndia
  4. 4.SRM Research InstituteSRM Institute of Science and TechnologyChennaiIndia

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