Capsaicin but not Genistein Influences Modulation of Lipid Parameters by Obestatin in DIO-C57BL/6 Mice

  • Musunuru Suneel Kumar Reddy
  • Uma V. ManjapparaEmail author


Capsaicin from chillies and genistein from soya bean have been shown to reduce cholesterol and triglyceride levels effectively. This study was done to test the hypothesis if these nutraceuticals could enhance reduction in body weight in combination with a satiety peptide namely obestatin produced in the stomach. It brings about satiety mainly by reducing jejunal motility by signalling through the hypothalamus. Significant reduction in food intake and moderate reduction in gain in body weight was observed upon intraperitoneal administration in rodents. We aimed to explore if co-administration with capsaicin or genistein could further enhance the ability of obestatin to reduce gain in body weight in Diet-Induced-Obese mice. After 18 weeks on high fat diet, 22-week-old C57BL/6 mice were randomly grouped, 6 mice in each group with an average weight of 44 ± 3 g. 0.5 mg/kgBW capsaicin, 2 mg/kgBW genistein, 160 nmol/kgBW obestatin in 20% DMSO-saline was administered either individually or pairwise with obestatin, at the same time every day for a period of 8 days. Co-administration of obestatin and capsaicin showed increased reduction in food intake, gain in body weight, plasma and adipose triglycerides. Whereas, co-administration with genistein showed no enhanced effect on the parameters studied. mRNA profiling and western blot analysis of epididymal adipose tissue revealed upregulation of peroxisome proliferator-activated protein γ and DiacylGlycerol AcylTransferase1 by capsaicin + obestatin and adipose triglyceride lipase by capsaicin. This opens up the possibility of using multi-pronged approaches to counter obesity through food.


Capsaicin Obesity Obestatin Epidydimal fat C57BL/6 mice 



Diet induced obesity


High fat diet


Body weight




Total cholesterol


High density lipoprotein-cholesterol


Alkaline phosphatase


Aspartate transaminase


Alanine transaminase


Obestatin + genistein


Obestatin + capsaicin


Glyceraldehyde 3-phosphate dehydrogenase


Sterol regulatory element binding transcription factor 1


Peroxisome proliferator-activated receptor gamma


Acetyl-CoA carboxylase alpha


Diacylglycerol O-acyltransferase 1


Hormone-sensitive lipase


Fatty acid synthase


Adipose triglyceride lipase


Lipoprotein lipase



This work was financially supported under the BSC-0202 (WELFO) project of CSIR-CFTRI, funded by the Council of Scientific & Industrial Research, India (CSIR). MSKR would like to acknowledge CSIR for providing fellowship (31/005(0514)/2012-EMR-1). Authors would like to acknowledge R.S Govardhan Singh for helping in manuscript preparation.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflicts of interest.

Supplementary material

10989_2019_9811_MOESM1_ESM.docx (3.3 mb)
Supplementary material 1 (DOCX 3361 KB)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Musunuru Suneel Kumar Reddy
    • 1
    • 2
  • Uma V. Manjappara
    • 1
    • 2
    Email author
  1. 1.Department of Lipid ScienceCSIR-Central Food Technological Research Institute (CFTRI)MysoreIndia
  2. 2.Academy of Scientific & Innovative ResearchCSIR-Central Food Technological Research Institute (CFTRI)MysoreIndia

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