Xenobiotic Binding Domain of Glutathione S-Transferase Has Cryptic Antimicrobial Peptides

  • Soyar Horam
  • Sneha Raj
  • Vikash Chandra Tripathi
  • Garima Pant
  • Mitra Kalyan
  • Thota Jagadeshwar Reddy
  • Jesu Arockiaraj
  • Mukesh PasupuletiEmail author


Antimicrobial peptides are one of the important components of innate immune defense system and play a critical role in controlling infections. Although vast sequence and structural diversities exist, AMPs share several common features like cationicity, amphipathicity and membrane permeabilisation as mode of action. In this study, a moderately short cationic and hydrophobic peptides derived from the conserved domains of human glutathione S-transferase (GST) have been shown to have antimicrobial activity against Staphylococcus aureus ATCC 29213 and Klebsiella pneumoniae ATCC 7637 in physiological conditions without any toxicity issues. We further shown here that human GST, a C-terminal region which showed the higher antimicrobial activity, is conserved in the vertebrates. Our results demonstrate the potential of human GST derived peptides as a template for the development of anti-infective therapeutics.


ESKAPE Glutathione S-transferase Antimicrobial peptides Host defence peptides S. aureus K. pneumonia 



MP is grateful to the Director, CDRI, Lucknow, India for providing the seed grant to establish the group and constant encouragement during the study. MP is thankful to DST-SERB, New Delhi [grant no.: SB/YS/LS-321/2013] for financial assistance. SH and VCT are grateful to UGC, India for the financial support. SR is thankful to ICMR, New Delhi, India for senior research fellowship. We are very thankful to RK Purshottam, SAIF, CSIR-CDRI for recording the flow cytometry profiles. We great fully acknowledge Mr A.Lal for technical support. CSIR-CDRI communication number: 9779.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Microbiology DivisionCSIR-Central Drug Research InstituteLucknowIndia
  2. 2.Electron Microscopy UnitCSIR-Central Drug Research InstituteLucknowIndia
  3. 3.Mass Spectrometry Laboratory, Sophisticated Analytical Instrument FacilityCSIR-Central Drug Research InstituteLucknowIndia
  4. 4.SRM Research InstituteSRM Institute of Science and TechnologyChennaiIndia

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