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Journal of Muscle Research and Cell Motility

, Volume 40, Issue 3–4, pp 389–398 | Cite as

CaATP prolongs strong actomyosin binding and promotes futile myosin stroke

  • Jinghua Ge
  • Akhil Gargey
  • Irina V. Nesmelova
  • Yuri E. NesmelovEmail author
Original Paper

Abstract

Calcium plays an essential role in muscle contraction, regulating actomyosin interaction by binding troponin of thin filaments. There are several buffers for calcium in muscle, and those buffers play a crucial role in the formation of the transient calcium wave in sarcomere upon muscle activation. One such calcium buffer in muscle is ATP. ATP is a fuel molecule, and the important role of MgATP in muscle is to bind myosin and supply energy for the power stroke. Myosin is not a specific ATPase, and CaATP also supports myosin ATPase activity. The concentration of CaATP in sarcomeres reaches 1% of all ATP available. Since 294 myosin molecules form a thick filament, naïve estimation gives three heads per filament with CaATP bound, instead of MgATP. We found that CaATP dissociates actomyosin slower than MgATP, thus increasing the time of the strong actomyosin binding. The rate of the basal CaATPase is faster than that of MgATPase, myosin readily produces futile stroke with CaATP. When calcium is upregulated, as in malignant hyperthermia, kinetics of myosin and actomyosin interaction with CaATP suggest that myosin CaATPase activity may contribute to observed muscle rigidity and enhanced muscle thermogenesis.

Keywords

Myosin Muscle ATP Calcium Malignant hyperthermia Transient kinetics 

Notes

Acknowledgements

This work was supported by National Institutes of Health (Grant No. HL132315) and by funds provided by the University of North Carolina at Charlotte.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest with the contents of this article.

Ethical approval

Myosin and actin were produced from rabbit skeletal tissue. All experimental protocols were approved by the Institutional Animal Care and Use Committee of UNC Charlotte and all experiments were performed in accordance with relevant guidelines and regulations.

Supplementary material

10974_2019_9556_MOESM1_ESM.docx (120 kb)
Supplementary material 1 (DOCX 120 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Jinghua Ge
    • 1
    • 2
  • Akhil Gargey
    • 1
    • 2
    • 3
  • Irina V. Nesmelova
    • 1
    • 2
  • Yuri E. Nesmelov
    • 1
    • 2
    Email author
  1. 1.Department of Physics and Optical ScienceUniversity of North Carolina CharlotteCharlotteUSA
  2. 2.Center for Biomedical Engineering and ScienceUniversity of North CarolinaCharlotteUSA
  3. 3.Department of Biological ScienceUniversity of North Carolina CharlotteCharlotteUSA

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