Cold shock protein RBM3 attenuates atrophy and induces hypertrophy in skeletal muscle

  • Douglas W. Van Pelt
  • Amy L. Confides
  • Andrew R. Judge
  • Peter W. Vanderklish
  • Esther E. Dupont-VersteegdenEmail author
Rapid Communication


RNA-binding motif protein 3 (RBM3), a stress-inducible RNA-binding protein that increases protein synthesis and confers cell protection in multiple cell types, has been identified as a possible regulator of skeletal muscle mass. Therefore, the primary aim of this study was to examine the impact of elevated RBM3 on skeletal muscle hypertrophy and resistance to atrophy. Plasmid-mediated overexpression of RBM3 in vitro and in vivo was used to assess the role of RBM3 in muscle. C2C12 myotubes overexpressing RBM3 were approximately 1.6 times larger than non-transfected myotubes, suggesting a role for RBM3 in hypertrophy. In addition, elevated RBM3 attenuated atrophy in myotubes exposed to dexamethasone. In agreement with in vitro results, overexpression of RBM3 in soleus muscle of F344/BN rats using electroporation techniques increased the cross sectional area of muscle fibers. Overexpression of RBM3 also attenuated muscle atrophy in rat soleus muscle undergoing disuse atrophy. These findings provide direct evidence for a novel role of RBM3 in inducing hypertrophy as well as attenuating atrophy.


Skeletal muscle atrophy Skeletal muscle hypertrophy RNA binding proteins Translation 



This work was supported by NIH Grant AG028925 to ED-V, and NIH Grant R01NS066053 to PWV.


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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Douglas W. Van Pelt
    • 1
    • 2
  • Amy L. Confides
    • 1
    • 2
  • Andrew R. Judge
    • 3
  • Peter W. Vanderklish
    • 4
  • Esther E. Dupont-Versteegden
    • 1
    • 2
    Email author
  1. 1.Department Rehabilitation Sciences, College of Health SciencesUniversity of KentuckyLexingtonUSA
  2. 2.Center for Muscle BiologyUniversity of KentuckyLexingtonUSA
  3. 3.Department of Physical Therapy, College of Public Health and Health ProfessionsUniversity of FloridaGainesvilleUSA
  4. 4.Department of Molecular MedicineThe Scripps Research InstituteLa JollaUSA

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