Microcalorimetric evaluation of the effects of three anthraquinone derivatives from Chinese Rhubarb and the synergistic effect of the mixture on Staphylococcus aureus
- 10 Downloads
In this study, a noninvasive and nondestructive microcalorimetric method was used to investigate the antimicrobial activity of three anthraquinone derivatives (emodin, aloe-emodin and physcion) from Chinese Rhubarb. Additionally, we observed a synergistic antibacterial effect of a mixture (emodin + aloe-emodin) on Staphylococcus aureus. Antibacterial effects were further evaluated through principle component analysis and the half-inhibitory concentration (IC50) according to the influence of the anthraquinone derivatives on eight quantitative thermokinetic parameters, which were measured by isothermal microcalorimetry and obtained from metabolic power–time curves of Staphylococcus aureus growth at 37 °C. The inhibitory actions of the anthraquinone derivatives varied at different concentrations. The antibacterial effect of the derivatives on S. aureus was as follows: emodin + aloe-emodin (E + AE) > emodin (E) > aloe-emodin (AE) > physcion. Based on these results, the combined effect of emodin and aloe-emodin was stronger than that of each anthraquinone derivative alone. The combination of emodin and aloe-emodin is a promising antibacterial agent, providing a novel avenue for antibacterial materials.
KeywordsEmodin Aloe-emodin Physcion Antibacterial Synergistic S. aureus
This study was supported by Research Project of Liaoning Provincial Department of Education (No. JYTFW201915). Xiangka Hu acknowledges their team for their help.
Compliance with ethical standards
Conflict of interest
The authors declare no conflicts of interest.
- 16.Siri M, Ruocco MJF, Achilli E, Pizzuto M, Delgado JF, Ruysschaert JM, et al. Effect of structure in ionised albumin based nanoparticle: characterisation, Emodin interaction, and in vitro cytotoxicity. Mater Sci Eng C Mater Biol Appl. 2019. https://doi.org/10.1016/j.msec.2019.109813.CrossRefPubMedGoogle Scholar
- 20.Wang T, Zhou X, Zou W, Zhang P, Wang J, Li H, et al. Synergistic effects of Ginseng C. A. Mey and Astragalus membranaceus (Fisch.) Bunge on activating mice splenic lymphocytes detected by microcalorimetry and the underlying mechanisms predicted by in silico network analysis. J Therm Anal Calorim. 2018;132:1933–42.CrossRefGoogle Scholar
- 32.Yi LZ, Yuan DL, Liang YZ, Xie PS, Zhao Y. Quality control and discrimination of pericarpium citri reticulatae and pericarpium citri reticulatae viride based on high-performance liquid chromatographic fingerprints and multivariate statistical analysis. Anal Chim Acta. 2007;588:207–15.CrossRefGoogle Scholar
- 38.Schmidt T, Kock MM, Ehlers MM. Molecular characterization of staphylococcus aureus isolated from bovine mastitis and close human contacts in south african dairy herds: genetic diversity and inter-species host transmission. Front Microbiol. 2017. https://doi.org/10.3389/fmicb.2017.00511.CrossRefPubMedPubMedCentralGoogle Scholar