Microcalorimetric study on the activation effects of Salviae miltiorrhizae combined with Radix puerariae on mice splenic lymphocytes

  • Xiangbo Meng
  • Xuelin Zhou
  • Tao Wang
  • Fengyi Li
  • Haotian Li
  • Jianyu Li
  • Ruilin Wang
  • Shizhang Wei
  • Yaming Zhang
  • Huandan Cai
  • Chunmei DaiEmail author
  • Yanling ZhaoEmail author


The thermogenic activity of Radix puerariae, Salvia miltiorrhiza and S. miltiorrhiza + R. puerariae on the spleen lymphocytes of mice was investigated by using isothermal microcalorimetry. By analyzing the area under the curve, a good dose–effect relationship was obtained for R. puerariae, S. miltiorrhiza and S. miltiorrhiza + R. puerariae, respectively. R. puerariae group and S. miltiorrhiza group expressed a synergistic effect on the spleen lymphocytes between R. puerariae and S. miltiorrhiza. The effect is in a high-dose-dependent relationship. Group of the S. miltiorrhiza + R. puerariae combination was significantly better than the effect of low-dose group, in line with the clinical drug rule. The activation mechanism was explored by network pharmacology method. The 20 pathways which S. miltiorrhiza and R. puerariae work together may be the basis for their synergistic effects. The combined application of microcalorimetry and network pharmacology of traditional Chinese medicine provides new ideas and research directions for traditional Chinese medicine researches.


Microcalorimetry Splenic lymphocytes Salviae miltiorrhizae Radix puerariae Synergistic effect Network pharmacology 



This study was supported by Foundation of National Major Drug Discovery Project (No. 2015ZX09J15102-004), Science and technology project of Liaoning Province (NO. 201602275).


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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2019

Authors and Affiliations

  1. 1.College of PharmacyJinzhou Medical UniversityJinzhouChina
  2. 2.Department of Pharmacy302 Military Hospital of ChinaBeijingChina
  3. 3.Treatment and Research Center for Infectious Diseases302 Hospital of P.L.A.BeijingChina
  4. 4.Integrative Medical Center302 Military Hospital of ChinaBeijingChina
  5. 5.China Military Institute of Chinese Medicine302 Military Hospital of ChinaBeijingChina

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