Journal of Thermal Analysis and Calorimetry

, Volume 134, Issue 3, pp 1929–1941 | Cite as

Compatibility study of tobramycin and pharmaceutical excipients using differential scanning calorimetry, FTIR, DRX, and HPLC

  • María A. Rosasco
  • Silvina L. Bonafede
  • Sonia N. Faudone
  • Adriana I. SegallEmail author


Differential scanning calorimetry (DSC), isothermal stress testing–Fourier transform infrared spectroscopy (IST–FTIR), isothermal stress testing–high-performance liquid chromatography, and powder X-ray diffraction (PDRX) were used as screening techniques for assessing the compatibility of tobramycin with some currently employed ophthalmic excipients. In the first phase of the study, DSC was used as a tool to detect any interaction. The absolute value of the difference between the enthalpy of the pure tobramycin melting peak and that of its melting peak in the different analyzed mixtures was chosen as a parameter of the drug–excipient interaction degree. DSC results demonstrated that benzalkonium chloride, monobasic sodium phosphate, boric acid, edetate disodium, sodium metabisulfite, thimerosal, and potassium sorbate interact with tobramycin. Taking into account these results, it could be suggested that some of the changes observed in the IST–FTIR spectra of binary blends of tobramycin and some of the excipients would account for a possible interaction between the mixture component. In this study, PDRX did not provide much information, since only tobramycin–thimerosal interactions could be detected. DSC and IST–FTIR are suitable and simple methods for the detection of potential incompatibilities between active pharmaceutical ingredient (API) and excipients.


Compatibility studies Tobramycin DSC IST–FTIR PDRX IST–HPLC 



This work was supported by Grant 20020130100342BA from UBA to A. I. Segall.


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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2018

Authors and Affiliations

  1. 1.Facultad de Farmacia y Bioquímica, Cátedra de Calidad de Medicamentos, CONICETUniversidad de Buenos AiresBuenos AiresArgentina
  2. 2.CEPROCOR Centro de Excelencia en Productos y Procesos de CórdobaCórdobaArgentina

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