The impact of copper oxide nanoparticles on the structure and applicability of bioactive glasses
- 22 Downloads
In order to identify novel appropriate compositions for targeted tissue engineering applications, bioactive glasses with copper oxide content were synthesized by sol-gel method and further investigated. Copper oxide nanoparticles (Cu2ONP) were chosen with the aim of understanding the impact of different copper species on the biocompatibility and antibacterial activity. The structural analyses revealed that the copper in the glass samples is in the copper hydroxy phosphate form, i.e., Cu2(PO4)(OH). The in vitro bioactivity assessment revealed that in the simulated body fluid were leached ionic species as CuH2PO4+, which interact with biological materials. In vitro cells viability assays performed with Human keratinocytes cells show good proliferation rate for the sample with 0.2 mol% CuO. The good antibacterial effect was obtained against Pseudomonas aeruginosa using glasses with 0.2–0.5 mol% CuO, and against Staphylococcus aureus, where only the sample with 0.5 mol% CuO exhibited a slight effect.
Copper oxide nanoparticles (Cu2ONP) used in sol-gel derived bioactive glasses.
Hydroxy phosphate form, i.e., Cu2(PO4)(OH) is formed in the glass samples.
In the simulated body fluid were leached ionic species as CuH2PO4+.
Good proliferation rate on Human keratinocytes cells were obtained for the sample with 0.2 mol% CuO.
The good antibacterial effect was obtained against Pseudomonas aeruginosa.
Only Cu2+ is not sufficient for the good antibacterial effect.
KeywordsBioactive glasses Copper oxide nanoparticles Cell viability Antibacterial activity
This work was supported by a grant of Ministry of Research and Innovation, CNCS—UEFISCDI, project number PN-III-P1-1.1-TE-2016-1324, within PNCDI III.K. Magyari wishes to thank for the financial support provided by MTA Domus937/27/2016/HTMT.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 7.Kargozar S, Baino F, Hamzehlou S, Hill RG, Mozafari M (2018) Bioactive glasses: sprouting angiogenesis in tissue engineering. Trends Biotechnol 6(4):430–444Google Scholar
- 18.Popescu RA, Magyari K, Taulescu M, Vulpoi A, Berce C, Bogdan S, Lelescu C, Dreanca A, Tudoran O, Papuc I, Baia L (2018) New alginate-pullulan-bioactive glasses composites with copper oxide for bone tissue regeneration trials. J Tissue Eng Regen Med 12:2112–2121Google Scholar
- 20.Popescu RA, Magyari K, Vulpoi A, Trandafir DL, Licarete E, Todea M, Stefan R, Voica C, Vodnar DC, Simon S, Papuc I, Baia L (2016) Bioactive and biocompatible copper containing glass-ceramics with remarkable antibacterial properties and high cell viability designed for future in vivo trials. Biomater Sci 4:1252–1265CrossRefGoogle Scholar
- 22.Usman MS, El Zowalaty ME, Shameli K, Zainuddin N, Salama M, Ibrahim NA (2013) Synthesis, characterization, and antimicrobial properties of copper nanoparticles. Int J Nanomed 8:4467–4479Google Scholar
- 41.Downs R (2006) The RRUFF project: an integrated study of the chemistry, crystallography, Raman and infrared spectroscopy of minerals, Program and Abstracts of the 19th General Meeting of the International Mineralogical Association in Kobe, Japan. http://rruff.info/chem=O,Ag/display=default/