Engineering of large-pore lipid-coated mesoporous silica nanoparticles for dual cargo delivery to cancer cells
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Lipid-coated mesoporous silica nanoparticles (LC-MSNs) have recently emerged as a next-generation cargo delivery nanosystem combining the unique attributes of both the organic and inorganic components. The high surface area biodegradable inorganic mesoporous silica core can accommodate multiple classes of bio-relevant cargos in large amounts, while the supported lipid bilayer coating retains the cargo and increases the stability of the nanocarrier in bio-relevant media which should promote greater bio-accumulation of LC-MSNs in cancer sites. In this contribution, we report on the optimization of various sol–gel synthesis (pH, stirring speed) and post-synthesis (hydrothermal treatment) procedures to enlarge the MSN pore size and tune the surface chemistry so as to enable loading and delivery of large biomolecules. The proof of concept of the dual cargo-loaded nanocarrier has been demonstrated in immortalized cervical cancer HeLa cells using MSNs of various fine-tuned pore sizes.
Lipid-coated mesoporous silica nanoparticles were prepared for dual cargo delivery to cancer cells.
The pore and particle sizes, surface areas, and condensation degrees were tuned by sol–gel processes.
Sol–gel (pH, stirring speed) and post-synthesis (hydrothermal treatment) parameters were optimized.
KeywordsMesoporous silica nanoparticles Large pore Sol–gel Supported lipid bilayer Drug delivery Biomedical
This work was supported by the Sandia National Laboratories' Laboratory Directed Research and Development (LDRD) program and the Lymphoma and Leukemia Society (LLS) (A.N., E.A.H., J.G.C., P.N.D., J.O.A. and C.J.B.). Sandia National Laboratories is a multimission laboratory managed and operated by National Technology & Engineering Solutions of Sandia, LLC, a wholly owned subsidiary of Honeywell International Inc., for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-NA0003525. This paper describes objective technical results and analysis. Any subjective views or opinions that might be expressed in the paper do not necessarily represent the views of the U.S. Department of Energy or the United States Government.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 2.Brinker CJ, Scherer GW (1990) Sol-gel science: the physics and chemistry of sol-gel processing. Academic Press, BostonGoogle Scholar
- 6.Durfee PN, Lin YS, Dunphy DR, Muñiz AJ, Butler KS, Humphrey KR, Lokke AJ, Agola JO, Chou SS, Chen IM, Wharton W, Townson JL, Willman CL, Brinker CJ (2016) Mesoporous silica nanoparticle-supported lipid bilayers (protocells) for active targeting and delivery to individual leukemia cells. ACS Nano 10:8325–8345CrossRefGoogle Scholar
- 7.Shenoi-Perdoor S, Noureddine A, Dubois F, Wong Chi Man M, Cattoën X (2016) Click functionalization of sol–gel materials. Handbook of sol-gel science and technology. Springer, Cham, pp 1–40Google Scholar
- 8.Noureddine A, Lichon L, Maynadier M, Garcia M, Gary-Bobo M, Zink JI, Cattoen X, Wong Chi Man M (2015) Controlled multiple functionalization of mesoporous silica nanoparticles: homogeneous implementation of pairs of functionalities communicating through energy or proton transfers. Nanoscale 7:11444–11452CrossRefGoogle Scholar
- 17.Fatieiev Y, Croissant J, Alamoudi K, Khashab N (2017) Cellular internalization and biocompatibility of periodic mesoporous organosilica nanoparticles with tunable morphologies: from nanospheres to nanowires. ChemPlusChem 82:631–637Google Scholar
- 22.Sun B, Pokhrel S, Dunphy DR, Zhang H, Ji Z, Wang X, Wang M, Liao YP, Chang CH, Dong J, Li R, Mädler L, Brinker CJ, Nel AE, Xia T (2015) Reduction of acute inflammatory effects of fumed silica nanoparticles in the lung by adjusting silanol display through calcination and metal doping. ACS Nano 9:9357–9372CrossRefGoogle Scholar
- 23.Zhang H, Dunphy DR, Jiang X, Meng H, Sun B, Tarn D, Xue M, Wang X, Lin S, Ji Z, Li R, Garcia FL, Yang J, Kirk ML, Xia T, Zink JI, Nel A, Brinker CJ (2012) Processing pathway dependence of amorphous silica nanoparticle toxicity: colloidal vs pyrolytic. J Am Chem Soc 134:15790–15804CrossRefGoogle Scholar
- 29.Omar H, Croissant JG, Alamoudi K, Alsaiari S, Alradwan I, Majrashi MA, Anjum DH, Martins P, Laamarti R, Eppinger J, Moosa B, Almalik A, Khashab NM (2017) Biodegradable magnetic silica@iron oxide nanovectors with ultra-large mesopores for high protein loading, magnetothermal release, and delivery. J Control Rel 259:187–194CrossRefGoogle Scholar
- 37.Croissant JG, Zhang D, Alsaiari S, Lu J, Deng L, Tamanoi F, AlMalik AM, Zink JI, Khashab NM (2016) Protein-gold clusters-capped mesoporous silica nanoparticles for high drug loading, autonomous gemcitabine/doxorubicin co-delivery, and in-vivo tumor imaging. J Control Rel 229:183–191CrossRefGoogle Scholar
- 40.Ashley CE, Carnes EC, Phillips GK, Padilla D, Durfee PN, Brown PA, Hanna TN, Liu J, Phillips B, Carter MB, Carroll NJ, Jiang X, Dunphy DR, Chackerian CL, Wharton W, Peabody DS, Brinker CJ (2011) The targeted delivery of multicomponent cargos to cancer cells by nanoporous particle-supported lipid bilayers. Nat Mater 10:389–397CrossRefGoogle Scholar
- 47.Slaughter BV, Lino CA, McBride AA, Fleig PF, Conroy MA, Melo CF, Wilkinson BS, Garcia GU, Wu TU, Adolphi NU, Reed S (2016) Mesoporous silica nanoparticle-supported lipid bilayers for targeted antibiotic therapeutics. Sandia National Laboratories (SNL-NM), Albuquerque, NMGoogle Scholar
- 48.Dengler EC, Liu J, Kerwin A, Torres S, Olcott CM, Bowman BN, Armijo L, Gentry K, Wilkerson J, Wallace J, Jiang X, Carnes EC, Brinker CJ, Milligan ED (2013) Mesoporous silica-supported lipid bilayers (protocells) for DNA cargo delivery to the spinal cord. J Control Rel 168:209–224CrossRefGoogle Scholar