Journal of Sol-Gel Science and Technology

, Volume 88, Issue 1, pp 1–5 | Cite as

Effect of polyethylene glycol additives on structure, stability, and biocatalytic activity of ormosil sol–gel encapsulated yeast cells

  • D. G. Lavrova
  • O. A. Kamanina
  • A. V. Machulin
  • N. E. Suzina
  • V. A. Alferov
  • O. N. PonamorevaEmail author
Original Paper: Sol-gel and hybrid materials for biological and health (medical) applications


Biohybrid materials based on ormosil encapsulated yeast cells were synthesized through a one-step sol–gel route with base-catalyst (NaF) using tetraethoxysilane (TEOS), methyltriethoxysilane (MTES) and polyethylene glycol (PEG) with different molar weights as a structure-controlling agent. Phase contrast microscopy and scanning electron microscopy were employed to evidence possible structures of the materials. The addition of PEG during cell encapsulation has induced structural changes within the biohybrids, which depend on PEG molecular weights. The biocatalytic activity of the living hybrids has been investigated by a biosensor which was based on the Clark-type oxygen electrode.

Graphical Abstract

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sol–gel ORMOSIL PEG Encapsulated cells Biocatalyst Biosensor 



The reported study was funded by Russian Foundation for Basic Research and Tula Region Government according to the research project No. 16-43-710183.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

10971_2017_4333_MOESM1_ESM.eps (6.1 mb)
Supplementary Information
10971_2017_4333_MOESM2_ESM.doc (1.2 mb)
Supplementary Figure S1


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Tula State UniversityTulaRussia
  2. 2.Laboratory of Cytology of MicroorganismsG.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of SciencesPushchinoRussia

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