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Journal of Sol-Gel Science and Technology

, Volume 88, Issue 1, pp 1–5 | Cite as

Effect of polyethylene glycol additives on structure, stability, and biocatalytic activity of ormosil sol–gel encapsulated yeast cells

  • D. G. Lavrova
  • O. A. Kamanina
  • A. V. Machulin
  • N. E. Suzina
  • V. A. Alferov
  • O. N. Ponamoreva
Original Paper: Sol-gel and hybrid materials for biological and health (medical) applications

Abstract

Biohybrid materials based on ormosil encapsulated yeast cells were synthesized through a one-step sol–gel route with base-catalyst (NaF) using tetraethoxysilane (TEOS), methyltriethoxysilane (MTES) and polyethylene glycol (PEG) with different molar weights as a structure-controlling agent. Phase contrast microscopy and scanning electron microscopy were employed to evidence possible structures of the materials. The addition of PEG during cell encapsulation has induced structural changes within the biohybrids, which depend on PEG molecular weights. The biocatalytic activity of the living hybrids has been investigated by a biosensor which was based on the Clark-type oxygen electrode.

Graphical Abstract

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Keywords

sol–gel ORMOSIL PEG Encapsulated cells Biocatalyst Biosensor 

Notes

Acknowledgements

The reported study was funded by Russian Foundation for Basic Research and Tula Region Government according to the research project No. 16-43-710183.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

10971_2017_4333_MOESM1_ESM.eps (6.1 mb)
Supplementary Information
10971_2017_4333_MOESM2_ESM.doc (1.2 mb)
Supplementary Figure S1

References

  1. 1.
    Ismail WNW (2016) Sol–gel technology for innovative fabric finishing—a review. J Sol-Gel Sci Technol 78(3):698–707CrossRefGoogle Scholar
  2. 2.
    Boury B, Corriu RJP. (2002) Auto-organisation of hybrid organic—inorganic materials prepared by sol—gel process. Chem Commun (Camb) 8:795–802.Google Scholar
  3. 3.
    Pandey S, Mishra SB (2011) Sol-gel derived organic-inorganic hybrid materials: Synthesis, characterizations and applications. J Sol-Gel Sci Technol 59(1):73–94CrossRefGoogle Scholar
  4. 4.
    Ciriminna R et al. (2013) The sol-gel route to advanced silica-based materials and recent applications. Chem Rev 113(8):6592–6620CrossRefGoogle Scholar
  5. 5.
    Schmidt H, Wfirzburg D (1984) Organically modified silicates by the sol-gel process. Mater Res Soc Symp Proc 32:327–335CrossRefGoogle Scholar
  6. 6.
    Judeinstein P, Sanchez C (1996) Hybrid organic/inorganic materials: a land of multidisciplinarity. J Mater Chem 6(4):511–525CrossRefGoogle Scholar
  7. 7.
    Klein LC, Jitianu A (2010) Organic-inorganic hybrid melting gels. J Sol-Gel Sci Technol 55(1):86–93CrossRefGoogle Scholar
  8. 8.
    Ma Y, Kanezashi M, Tsuru T (2010) Preparation of organic/inorganic hybrid silica using methyltriethoxysilane and tetraethoxysilane as co-precursors. J Sol-Gel Sci Technol 53(1):93–99CrossRefGoogle Scholar
  9. 9.
    Owens GJ et al. (2016) Sol–gel based materials for biomedical applications: article. Prog Mater Sci 77:1–79CrossRefGoogle Scholar
  10. 10.
    Dickson D, Ely R (2013) Silica sol-gel encapsulation of cyanobacteria: lessons for academic and applied research. Appl Microbiol Biotechnol 97(5):1809–1819CrossRefGoogle Scholar
  11. 11.
    Grandi S et al. (2007) Characterisation of a new sol-gel precursor for a SiO2-rhodamine 6G hybrid class II material. J Sol-Gel Sci Technol 41(1):57–63CrossRefGoogle Scholar
  12. 12.
    Shilova OA (2013) Synthesis and structure features of composite silicate and hybrid TEOS-derived thin films doped by inorganic and organic additives. J Sol-Gel Sci Technol 68(3):387–410CrossRefGoogle Scholar
  13. 13.
    Vong MSW, Bazin N, Sermon PA (1997) Chemical modification of silica gels. J Sol-Gel Sci Technol 8(1):499–505Google Scholar
  14. 14.
    Ferrer ML et al. (2003) Biocompatible sol-gel route for encapsulation of living bacteria in organically modified silica matrixes. Chem Mater 15(19):3614–3618CrossRefGoogle Scholar
  15. 15.
    Veres R et al. (2016) Gamma irradiation effect on bioactive glasses synthesized with polyethylene-glycol template. Ceram Int 42(1, Part B):1990–1997CrossRefGoogle Scholar
  16. 16.
    Boehm HP (1980) The chemistry of silica, solubility, polymerization, colloid and surface properties, and biochemistry. Angew Chemie 92(4):328CrossRefGoogle Scholar
  17. 17.
    Niu X et al. (2013) “Fish-in-Net”, a novel method for cell immobilization of zymomonas mobilis. PLoS ONE 8(11):e79569. (1–11)CrossRefGoogle Scholar
  18. 18.
    Meunier CF, Dandoy P, Su BL (2010) Encapsulation of cells within silica matrixes: Towards a new advance in the conception of living hybrid materials. J Colloid Interface Sci 342(2):211–224CrossRefGoogle Scholar
  19. 19.
    Ponamoreva ON et al. (2015) Yeast-based self-organized hybrid bio-silica sol–gels for the design of biosensors. Biosens Bioelectron 67:321–326CrossRefGoogle Scholar
  20. 20.
    Kamanina OA et al. (2014) Synthesis of organosilicon sol-gel matrices and preparation of heterogeneous biocatalysts based on them. Russ J Appl Chem 87(6):761–766CrossRefGoogle Scholar
  21. 21.
    Kandimalla V, Tripathi V, Ju H (2006) Immobilization of biomolecules in sol–gels: biological and analytical applications. Crit Rev Anal Chem 36(2):73–106CrossRefGoogle Scholar
  22. 22.
    Thevenot DR, Toth K, Durst RA, Wilson GS (2001) Electrochemical biosensors: recommended definitions and classification. Biosens Bioelectron 16:121–131CrossRefGoogle Scholar
  23. 23.
    Kamanina OA et al. (2016) Silica sol-gel encapsulated methylotrophic yeast as filling of biofilters for the removal of methanol from industrial wastewater. Enzyme Microb Technol 92:94–98CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Tula State UniversityTulaRussia
  2. 2.Laboratory of Cytology of MicroorganismsG.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of SciencesPushchinoRussia

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