Development and initial characterization of 99mTc labeled N-acetyl neuraminic acid for its application in in-vivo imaging of cancer: a preclinical study
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In the present study, N-acetyl neuraminic acid (Neu5Ac) was labeled with 99mTc and its bioevaluation was performed. Radiolabeling of Neu5Ac was obtained with a maximum labeling yield of 93.6% and was confirmed by instant thin layer chromatography, mass spectra (ESI-MS) and high performance liquid chromatography. The resulting radiocomplex showed hydrophilic properties and stability up to 8 h at room temperature in rat serum and saline. Biodistribution and scintigraphic studies exhibited accumulation of activity in the colon of tumor bearing rats. In conclusion, [99mTc]Tc-N-acetyl neuraminic acid exhibits selectivity for neoplastic colon tissue.
KeywordsSialic acid Radiolabeling Radionuclide imaging Biodistribution Technitium-99m Experimentally induced colon tumor
The authors thank to post graduate institute of medical research (PGIMER), Chandigarh India, for providing radioisotope.
This work is supported by Research Promotion Fund, Panjab University Alumni Association and DST (PURSE), India.
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