Neutron activation analysis as a tool for tracing the accumulation of silver nanoparticles in tissues of female mice and their offspring
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The silver accumulation in different tissues of female mice and their offspring after prolonged oral administration of silver nanoparticles to the females during pregnancy and lactation was investigated. Silver content in different organs (blood, liver, brain, kidney and lungs) was determined by means of neutron activation analysis. According to the obtained data silver nanoparticles are able to reach and cross the placental barrier and blood-to-brain barrier in both mice female and their offspring. In mice female the highest silver concentration was determined in lungs, followed by brain, liver, kidney and blood. In offspring silver bioaccumulation changed in the following order lungs > brain > blood > liver > kidney. The average specific mass content of silver which crossed the blood–brain barrier was 373 ± 75 ng (for female) and 385 ± 57 ng (for offspring). The obtained results are important for studies in developmental and reproductive toxicity of nanomaterials.
KeywordsSilver nanoparticles Brain Liver Lungs Kidney Blood Mice Offspring Distribution Neutron activation analysis
This work was supported by the Russian Foundation for Basic Research (RFBR) [Grant No. 19-015-00145 A].
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The experiments involving mice had been approved by local Ethics Committee and met the requirements of the Directive 2010/63/EU of the European Parliament and of the Council from September 22, 2010 on protection of animals used for scientific purposes and in compliance with the American College of Toxicology Policy on the Use of Animals in Toxicology.
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