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Journal of Radioanalytical and Nuclear Chemistry

, Volume 322, Issue 2, pp 629–638 | Cite as

Comparison of DOTA and NODAGA as chelates for 68Ga-labelled CDP1 as novel infection PET imaging agents

  • A. H. Mdlophane
  • T. Ebenhan
  • B. Marjanovic-Painter
  • T. Govender
  • M. M. Sathekge
  • J. R. ZeevaartEmail author
Article
  • 30 Downloads

Abstract

The cathelicidin-derived peptide (CDP1) is a human antimicrobial peptide that preferentially targets bacterial membranes in response to infection. CDP1 was functionalised with NODAGA and DOTA for complexation with gallium-68 to evaluate its potential as an infection imaging tracer. The synthesis of [68Ga]Ga–NODAGA–CDP1 and [68Ga]Ga–DOTA–CDP1 were optimised for pH, molarity, incubation time and temperature, and product purification. The integrity and protein binding were investigated employing [68Ga]GaCl3 and [68Ga]Ga–DOTA–TATE as internal references. [68Ga]Ga–NODAGA–CDP1 displayed good labelling properties with higher product yield compared to [68Ga]Ga–DOTA–CDP1. In contrast, [68Ga]Ga–DOTA–CDP1 showed better stability and is the preferred candidate for an in vivo investigation.

Keywords

CDP1 NODAGA DOTA 68Ga Antimicrobial peptides Infection imaging PET 

Notes

Acknowledgements

This work was supported by the Nuclear Technologies in Medicine and the Biosciences Initiative (NTeMBI), a national technology platform developed and managed by the South African Nuclear Energy Corporation (Necsa) and funded by the Department of Science and Technology (DST).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10967_2019_6693_MOESM1_ESM.docx (96 kb)
Supplementary material 1 (DOCX 95 kb)

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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2019

Authors and Affiliations

  • A. H. Mdlophane
    • 1
    • 2
  • T. Ebenhan
    • 1
    • 3
  • B. Marjanovic-Painter
    • 2
  • T. Govender
    • 4
    • 5
  • M. M. Sathekge
    • 1
    • 3
  • J. R. Zeevaart
    • 2
    • 3
    • 6
    Email author
  1. 1.Nuclear Medicine, University of Pretoria and Steve Biko Academic HospitalPretoriaSouth Africa
  2. 2.RadiochemistryThe South African Nuclear Energy Corporation (Necsa)BritsSouth Africa
  3. 3.Preclinical Imaging FacilityNuclear Medicine Research Infrastructure (NuMeRI)BritsSouth Africa
  4. 4.Catalysis and Peptide Research Unit, School of Health SciencesUniversity of KwaZulu-NatalDurbanSouth Africa
  5. 5.School of Chemistry and PhysicsUniversity of KwaZulu-NatalDurbanSouth Africa
  6. 6.Department of Science and Technology, Preclinical Drug Development PlatformNorth West UniversityPotchefstroomSouth Africa

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