Microarray immunoassay for thyrotropin on track-etched membranes using radiotracers

  • Bharti Jain
  • Savita Kulkarni
  • Sharmila Banerjee
  • M. G. Ramakrishna RajanEmail author


We report a microarray immunoassay for thyrotropin in serum using an anti-thyrotropin monoclonal ‘antibody-chip’ fabricated on polycarbonate track-etched membranes as a solid-support. The thyrotropin microarray immunoassay is an immunoradiometric assay using 125I tracer and requires very small volumes of sample and reagents. The microarray immunoassay had the required minimum detection level, intra- and inter-assay CV and working range to be clinically useful. It correlated very significantly with commercial immunoradiometric assays for thyrotropin. We developed this microarray immunoassay for thyrotropin since it is often used as the first line test for thyroid function.


Microarray immunoassay Thyroid stimulating hormone Track-etched membranes Antibody-chip 



The authors acknowledge the support from Dr. Deepak Modi, NIRRH, for allowing us to use Phosphorimager, at their institute.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standard

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.


  1. 1.
    Unnikrishnan AG, Menon UV (2011) Thyroid disorders in India: an epidemiological perspective. Indian J Endocrinol Metab 15:S78CrossRefGoogle Scholar
  2. 2.
    Vanderpump MPJ (2005) The epidemiology of thyroid diseases. In: Braverman LE, Utiger RD (eds) Werner and Ingbar’s the thyroid: a fundamental and clinical text, 9th edn. JB Lippincott-Raven, Philadelphia, pp 398–496Google Scholar
  3. 3.
    Spencer CA (2013) Thyroid function tests: assay of thyroid hormones and related substances. Comprehensive free online endocrinology textbook. Accessed 1 Jan 2013
  4. 4.
    Bernal J, Guadano-Ferraz A, Morte B (2003) Perspectives in the study of thyroid hormone action on brain development and function. Thyroid 13:1005–1012CrossRefGoogle Scholar
  5. 5.
    Korada M, Pearce MS, Avis E, Turner S, Cheetham T (2009) TSH levels in relation to gestation, birth weight and sex. Horm Res Paediatr 72:120–123CrossRefGoogle Scholar
  6. 6.
    Kaplan MM (1999) Clinical perspectives in the diagnosis of thyroid disease. Clin Chem 45:1377–1383Google Scholar
  7. 7.
    Ekins RP, Chu FW (1991) Multianalyte microspot immunoassay–microanalytical “compact disk” of the future. Clin Chem 37:1955–1967Google Scholar
  8. 8.
    Jain B, Kumarasamy J, Gholve C, Kulkarni S, Rajan MGR (2017) A microarray immunoassay for serum thyrotropin and thyroglobulin using antibodies immobilized on track-etched membranes. Indian J Clin Biochem 32:193–199CrossRefGoogle Scholar
  9. 9.
    Jain B, Kumarasamy J, Gholve C, Kulkarni S, Rajan MGR (2017) A multi-analyte immunoassay for thyroid related analytes. J Immunoass Immunochem 38:271–284CrossRefGoogle Scholar
  10. 10.
    Kros A, Nolte RJ, Sommerdijk NA (2002) Conducting polymers with confined dimensions: track-etch membranes for amperometric biosensor applications. Adv Mater 14:1779–1782CrossRefGoogle Scholar
  11. 11.
    Apel P (2001) Track etching technique in membrane technology. Radiat Meas 34:559–566CrossRefGoogle Scholar
  12. 12.
    Hanot H, Ferain E (2009) Industrial applications of ion track technology. Nucl Instrum Methods Phys Res B: Beam Interact Mater Atoms 267:1019–1022CrossRefGoogle Scholar
  13. 13.
    Seurynck-Servoss SL, White AM, Baird CL, Rodland KD, Zangar RC (2007) Evaluation of surface chemistries for antibody microarrays. Anal Biochem 371:105–115CrossRefGoogle Scholar
  14. 14.
    Romanov V, Davidoff SN, Miles AR, Grainger DW, Gale BK, Brooks BD (2014) A critical comparison of protein microarray fabrication technologies. Analyst 139:1303–1326CrossRefGoogle Scholar
  15. 15.
    Ylikotila J, Välimaa L, Vehniäinen M, Takalo H, Lövgren T, Pettersson K (2005) A sensitive TSH assay in spot-coated microwells utilizing recombinant antibody fragments. J Immunol Methods 306:104–114CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2019

Authors and Affiliations

  1. 1.Radiation Medicine Centre, Bhabha Atomic Research CentreParel, MumbaiIndia

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