Microarray immunoassay for thyrotropin on track-etched membranes using radiotracers
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We report a microarray immunoassay for thyrotropin in serum using an anti-thyrotropin monoclonal ‘antibody-chip’ fabricated on polycarbonate track-etched membranes as a solid-support. The thyrotropin microarray immunoassay is an immunoradiometric assay using 125I tracer and requires very small volumes of sample and reagents. The microarray immunoassay had the required minimum detection level, intra- and inter-assay CV and working range to be clinically useful. It correlated very significantly with commercial immunoradiometric assays for thyrotropin. We developed this microarray immunoassay for thyrotropin since it is often used as the first line test for thyroid function.
KeywordsMicroarray immunoassay Thyroid stimulating hormone Track-etched membranes Antibody-chip
The authors acknowledge the support from Dr. Deepak Modi, NIRRH, for allowing us to use Phosphorimager, at their institute.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed consent was obtained from all individual participants included in the study.
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