Advertisement

Preparation and SPECT imaging of the novel Anxa 1-targeted probe 99mTc-p-SCN-Bn-DTPA-GGGRDN-IF7

  • Fei Chen
  • Xiaojia Pu
  • Yichun Xiao
  • Kejing Shao
  • Junchao Wang
  • Wei Hu
  • Bao ZhuEmail author
  • Mengjun JiangEmail author
Article
  • 17 Downloads

Abstract

Annexin 1 (Anxa1) is a highly specific surface marker of tumor vasculature in several solid tumors. The IF7 peptide was modified with a hydrophic linkers,GGGRDN, and introduced into a new bifunctional chelating agent p-SCN-Bn-DTPA. The resulting peptides (p-SCN-Bn-DTPA-GGGRDN-IF7) was successfuly labeled with 99mTc. The targeting characteristics to Anxa1 of the radiolabeled peptide were evaluated by in vitro and in vivo study is on U87 tumor models. SPECT imaging revealed that the U87 tumors were clearly visualized (3.64 ± 0.44%ID/g at 2 h p.i.). 99mTc-p-SCN-Bn-DTPA-GGGRDN-IF7 (Tc-RIF7) might be a potential target probe for detecting Anxa1 levels in cancers due to the favorable characterizations such as convenient synthesis, specific Anxa1 targeting and moderate tumor uptakes.

Keywords

Annexin 1 GGGRDN-IF7 99mTc SPECT imaging 

Notes

Acknowledgements

We are grateful to this work is supported by Wuxi Young Medical Talents (QNRC012) and Wuxi Science and Technology Bureau Project (CSZ0N1603).

References

  1. 1.
    Chen WQ, Zheng RS, Baade PD, Zhang SW, Zeng HM, Bray F, Jemal A, Yu XQ, He J (2016) Cancer statistics in China 2015. CA Cancer J Clin 66(2):115–132CrossRefGoogle Scholar
  2. 2.
    Khondee S, Wang TD (2013) Progress in molecular imaging in endoscopy and endomicroscopy for cancer imaging. J Healthc Eng 4(1):1–22CrossRefGoogle Scholar
  3. 3.
    Lai WF, Rogach AL, Wong WT (2017) Chemistry and engineering of cyclodextrins for molecular imaging. Chem Soc Rev 46(20):6379–6419CrossRefGoogle Scholar
  4. 4.
    Kircher MF, Hricak H, Larson SM (2012) Molecular imaging for personalized cancer care. Mol Oncol 6(2):182–195CrossRefGoogle Scholar
  5. 5.
    Ahmed S, Strand S, Weinmann-Menke J, Urbansky L, Galle PR, Neumann H (2018) Molecular endoscopic imaging in cancer. Dig Endosc 30(6):719–729CrossRefGoogle Scholar
  6. 6.
    International Atomic Energy Agency (2008) Technetium-99 m radiopharmaceuticals: manufacture of kits. Technical Reports Series, Vienna, p 126Google Scholar
  7. 7.
    International Atomic Energy Agency (2008) Technetium-99 m radiopharmaceuticals: manufacture of kits. Technical Reports Series, Vienna, p 89Google Scholar
  8. 8.
    International Atomic Energy Agency (2008) Technetium-99 m radiopharmaceuticals: manufacture of kits. Technical Reports Series, Vienna, p 129Google Scholar
  9. 9.
    Baptiste GG, Postlewait LM, Ethun CG, Le N, McInnis MR, Russell MC, Winer JH, Kooby DA, Staley CA, Maithel SK, Cardona K (2016) Symptomatic presentation as a predictor of recurrence in gastroenteropancreatic neuroendocrine tumors: a single institution experience over 15 years. J Surg Oncol 114(2):163–169CrossRefGoogle Scholar
  10. 10.
    Gotthardt M, Behe MP (2003) Imaging lung tumors with peptide-based radioligands. Clin Lung Cancer 5(2):119–124CrossRefGoogle Scholar
  11. 11.
    Kwekkeboom D, Krenning EP, de Jong M (2000) Peptide receptor imaging and therapy. J Nucl Med 41(10):1704–1713Google Scholar
  12. 12.
    Sugawara K, Kuramitz H, Kadoya T (2018) Label-free cytosensing of cancer cells based on the interaction between protein and an electron-transfer carbohydrate-mimetic peptide. Anal Chim Acta 1040:166–176CrossRefGoogle Scholar
  13. 13.
    Hasib A, Ng MT, Khan D, Gault VA, Platt PR, Irwin N (2018) Characterisation and antidiabetic utility of a novel hybrid peptide, exendin-4/gastrin/xenin-8-Gln. Eur J Pharmacol 834:126–135CrossRefGoogle Scholar
  14. 14.
    Han G, Lu K, Huang J, Ye J, Dai S, Ye Y, Zhang L (2007) Effect of annexin A1 gene on the proliferation and invasion of esophageal squamous cell carcinoma cells and its regulatory mechanisms. Int J Mol Med 39(2):357–363CrossRefGoogle Scholar
  15. 15.
    Jia C, Kong D, Guo Y, Li L, Quan L (2018) Enhanced antitumor effect of combination of annexin A1 knockdown and bortezomib treatment in multiple myeloma in vitro and in vivo. Biochem Biophys Res Commun 505(3):720–725CrossRefGoogle Scholar
  16. 16.
    Raulf N, Lucarelli P, Thavaraj S, Brown S, Vicencio JM, Sauter T, Tavassoli M (2018) Annexin A1 regulates EGFR activity and alters EGFR-containing tumour-derived exosomes in head and neck cancers. Eur J Cancer 102:52–68CrossRefGoogle Scholar
  17. 17.
    Tadei MB, Mayorquim MV, de Souza CB, de Souza Costa S, Possebon L, Souza HR, Iyomasa-Pilon MM, Geromel MR, Girol AP (2018) Expression of the annexin A1 and its correlation with matrix metalloproteinases and the receptor for formylated peptide-2 in diffuse astrocytic tumors. Ann Diagn Pathol 37:62–66CrossRefGoogle Scholar
  18. 18.
    Gu XB, Jiang MJ, Pan DH, Cai GM, Zhang RJ, Zhou YY, Ding YM, Zhu B, Lin XF (2016) Preliminary evaluation of novel 18F-AlF-NOTA-IF7 as a tumor imaging agent. J Radioanal Nucl Chem 308:851–856CrossRefGoogle Scholar
  19. 19.
    Gu XB, Cai GM, Zhang RJ, Jiang MJ, Zhou YY, Pan DH, Xu YP, Huang HB (2015) Synthesis and preliminary evaluation of 68Ga-NOTA-IF7 as a tumor imaging agent. J Radioanal Nucl Chem 303:777–7782CrossRefGoogle Scholar
  20. 20.
    Honda M, Ishibashi M (2004) The diagnosis and treatment of insulinoma and gastrinoma. Gan To Kagaku Ryoho 31(3):337–341Google Scholar
  21. 21.
    Reigman HI, Stokkel MP (2004) Peripheral bone metastases in prostate cancer: a rare localization at initial presentation. Clin Nucl Med 29(5):335–336CrossRefGoogle Scholar
  22. 22.
    El-KawyOA Garcia-HorsmanJA (2017) Tc-99 m-roxifiban: a potential molecular imaging agent for the detection and localization of acute venous thrombosis. J Radioanal Nucl Chem 311(3):1719–1728CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2019

Authors and Affiliations

  1. 1.Department of Nuclear Medicine, Nanjing Medical UniversityAffiliated Wuxi People’s HospitalWuxiPeople’s Republic of China
  2. 2.Department of Clincial Laboratory Science, Nanjing Medical UniversityAffiliated Wuxi People’s HospitalWuxiPeople’s Republic of China

Personalised recommendations