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Evaluation of inhibitory effect of coptisine on protein kinase C activity using a RI detection-assisted biochip

  • Jung Ae Kang
  • Jong Kook Rho
  • Sang Hyun ParkEmail author
Article
  • 23 Downloads

Abstract

In this study, a radioisotope (RI) detection-assisted biochip was developed for the evaluation of the inhibitory effects of natural products on protein kinase C (PKC) activity. It was shown that PKC activity was inhibited by coptisine in dose-dependent manner. These findings suggest that the RI detection-assisted protein biochip should be applicable to high throughput screening for kinase inhibitors. It was found that coptisine shows inhibitory effect on PKC, and that the biochip can be used to rapidly screen kinase inhibitors of therapeutic agents.

Keywords

Protein kinase C Biochip Radioisotope High throughput screening Coptisine 

Notes

Acknowledgements

This work was supported by the Korea Atomic Energy Research Institute.

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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2019

Authors and Affiliations

  1. 1.Advanced Radiation Technology InstituteKorea Atomic Energy Research InstituteJeongupRepublic of Korea
  2. 2.Graduate School of Preclinical Laboratory ScienceKonyang UniversityDaejeonRepublic of Korea
  3. 3.Radiation Science and TechnologyUniversity of Science and TechnologyDaejeonRepublic of Korea
  4. 4.Laboratory Animal CenterOSONG Medical Innovation FoundationCheongjuRepublic of Korea
  5. 5.Repigen Co., Ltd.DaejeonRepublic of Korea

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