Application of multiple regression analysis in optimization of metronidazole-chitosan nanoparticles

  • Hazem Abdul Kader Sabbagh
  • Zead Abudayeh
  • Suha Mujahed Abudoleh
  • Jamal Alyousef Alkrad
  • Mohd Zobir Hussein
  • Samer Hasan Hussein-Al-AliEmail author


The current work aims to developing MET-CSNPs nanocomposites as drug delivery system. The nanocomposites were prepared by ionic interactions method and optimized using multiple regression analysis. Independent variables included chitosan concentration (CS), tri poly phosphate concentration (TPP) and metronidazole concentration (MET); while dependent variables were percentage loading drug (LE), zeta potential and zeta size. Prepared nanocomposites were characterized by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), scanning electron microscope (SEM) and in vitro drug release studies. TGA, FTIR and XRD studies indicated the presence of drug into final nanocomposites. In vitro drug release from nanocomposites was carried out and showed that the release rate of MET from the MET-CSNPs nanocomposites was very slow. These results indicate extended release of the drug from its respective nanocomposites, and therefore these nanocomposites have good potential to be used as extended-release formulation of the drugs.


Metronidazole Chitosan nanoparticles Multiple regression analysis Optimization 



This study was also supported by Hikma pharmaceuticals Research and development Department. Special thanks to “Ahmad Abu Sharar, R&D Project leader” for his insights and expertise that greatly assisted the researcher in Hikma Pharmaceuticals Co.


The author would like to thank the Faculty of Pharmacy at Isra University, and University Putra Malaysia under grand number (9443100 NANOMITE) provided funding for this research.

Compliance with ethical standards

Conflicting interests

The authors report no conflicts of interest in this work.


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Copyright information

© The Polymer Society, Taipei 2019

Authors and Affiliations

  1. 1.Faculty of PharmacyIsra UniversityAmmanJordan
  2. 2.Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology (ITMA)University Putra MalaysiaSeri KembanganMalaysia

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