Biodegradable dendrimer functionalized carbon nanotube-hybrids for biomedical applications
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In the present study, biodegradable poly(amidoamine) dendrimers (PAMAM G1/G2/G3) was covalently functionalized with carbon nanotubes (CNTs) and nanostructured hydroxyapatite was immobilized onto CNTs-PAMAM matrix through coordination bond between the –NH2 groups of dendrimer and Ca2+ of hydroxyapatite. The structural and morphological behaviors of the nanohybrids were established through spectroscopic and microscopic analyses. In vitro cytotoxicity and cell proliferation was assessed through osteoblast-like MG 63 cell line using 3–4, 5-dimethylthiazole-2-yl, 2,5-diphenyl tetrazolium bromide assay for 3 days. The more abundant of –NH2 group exist in PAMAM(G3) dendrimer attracts more number of HAp molecules onto their surface which exhibits enhanced activity in cell proliferations even at higher concentrations.
KeywordsCarbon nanotubes Dendrimer Hydroxyapatite Nanohybrids Bone tissue engineering
Authors acknowledge Prof.E.Murugan for his valuable suggestions to carry out this work and the National Centre for Nanoscience and Nanotechnology (NCNSNT), University of Madras for providing financial support & instrumentation facilities.
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