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PMAA nanogel controllably releases anti-IL-1β IgY for treating allergic rhinitis

  • Ting Wu
  • Tao Liu
  • Xiling Zhu
  • Yonghe Li
  • Xidong Wu
  • Mengying Xia
  • Zhixian Lv
  • Wenyun Zhou
  • Guozhu HuEmail author
  • Jinglin Zhang
  • Fenfen Wei
  • Shaobin Yu
  • Shaohua ChenEmail author
  • Xiang CaiEmail author
ORIGINAL PAPER
  • 4 Downloads

Abstract

Intranasal glucocorticoid spray is the preferred method to treat allergic rhinitis. However, due to the instantaneous high drug concentration, there are still some side effects, such as nasal dryness, nasal bleeding and even systemic effect of glucocorticoid. Herein, a novel biocompatible poly(methacrylic acid) (PMAA) nanogel was synthesized and used to load anti-IL-1β IgY (IgY) for treating allergic rhinitis induced by ovalbumin. The results indicated that PMAA nanogel had sustained-released effect of IgY in vitro. Symptoms of allergic rhinitis were remarkably relieved after the topical intranasal drip treatment using IgY/PMAA nanogel. The number of eosinophilic granulocyte in nasal lavage fluid and in nasal mucosa tissue, the expression of IL-1β analysis revealed that IgY/PMAA nanogel had a good controlled-released therapeutic effect, and IgY/PMAA nanogel exhibited lower in vivo toxicity in the experimental range, which suggested a promising potential for allergic rhinitis treatment with less side effect.

Keywords

Allergic rhinitis Poly(methacrylic acid) Anti-IL-1β IgY Nanogel Sustained-release 

Abbreviations

PMAA

Poly (methacrylic acid)

IL-1

Interleukin-1

IL-6

Interleukin-6

IL-8

Interleukin-8

Th2

T-helper 2 cells

GM-CSF

Granulocyte-macrophage colony stimulating factor

anti-IL-1β

Anti-Interleukin-1 beta

IgY

Immunoglobulin of yolk

IgE

Serum immunoglobulin

MUC5AC

Mucins 5 ac

In vitro

Within the glass

In vivo

Within the living

AR

Allergic rhinitis

OVA-induced

Ovalbumin-induced

FT-IR

Fourier transform infrared

SEM

Scanning electron microscopy

DLS

Dynamic laser light-scattering

MAA

Methacrylic acid

CH3CN

Acetonitrile

EGDMA

Ethylene glycol dimethacrylate

AIBN

2,2′-azobisisobutyronitrile

LC

Load content

LE

Load efficiency

WA

Water absorption

PBS

Phosphate Buffered Saline

NLF

Nasal lavage fluid

HE

Hematoxylin-eosin

Notes

Acknowledgements

The authors acknowledged financial support from the National Natural Science Foundation of China (81573000, 81260406, 21476052), the Foundation of Science and Technology Projects of Guangdong Province (2016A010103047, 2016A070711023), the Science and Technology Innovation Platform Project of Foshan City (2016AG100541, 2017AG100092), the Foshan Technology Research Center (2016GA10161), the talent introduction fund of Guangdong Provincial People’s Hospital (Y012018142), and the Project supported by GDHVPS (2017).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10965_2019_1846_MOESM1_ESM.doc (240 kb)
ESM 1 (DOC 240 kb)

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Copyright information

© The Polymer Society, Taipei 2019

Authors and Affiliations

  1. 1.Department of Light Chemical EngineeringGuangdong PolytechnicFoshanPeople’s Republic of China
  2. 2.Department of Otolaryngology-Head and Neck Surgery, Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhouChina
  3. 3.College of AgricultureAnshun UniversityAnshunChina
  4. 4.Department of Otolaryngology, Zhujiang HospitalSouthern Medical UniversityGuangzhouChina
  5. 5.Department of PharmacologyJiangxi Testing Center of Medical InstrumentsNanchangChina
  6. 6.Faculty of Basic Medicine, 2015 Grade, Zhongshan School of MedicineSun Yat-sen UniversityGuizhouChina
  7. 7.Institute of Clinical Medical SciencesJiangxi Province People’s HospitalNanchangChina
  8. 8.The No.1 Surgery Department of No.5 People’s Hospital of FoshanFoshanPeople’s Republic of China
  9. 9.Guangdong Gaoming Industrial Innovation Research InstituteFoshanPeople’s Republic of China

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