Solubility Determination of Hydroquinone in Dichloromethane, Trichloromethane and Carbon Tetrachloride by Using the Co-solvent Calibration Method
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In this report, the solubility of hydroquinone (1,4-dihydroxybenzene) was determined in dichloromethane, trichloromethane and carbon tetrachloride at 298 K by using cyclic voltammetry. By the proposed cosolvent calibration method a mixed solvent was used for the determination of solubility where acetonitrile was the co-solvent. The molar ratios of the two solvents were uniform during the whole procedure both in the calibration solutions and in solutions used for solubility determinations. The solubility of hydroquinone decreased by the decrease of solvent permittivity, which were in dichloromethane 5.683 mmol·L−1, in trichloromethane 2.785 mmol·L−1 and in carbon tetrachloride 0.321 mmol·L−1.
KeywordsSolubility Hydroquinone Cosolvent Chlorinated hydrocarbons
The knowledge of the solubility of materials in different solvents and solvent mixtures has great significance for many applications [1, 2, 3, 4, 5]. Numerous methods can provide a reliable solution for this problem. Gravimetry has had great interest for many years, which is based on the weighing of residual solid material . Particularly, in medical science dimethyl sulfoxide is a solvent which was added to the aqueous solution in low quantity facilitating the dissolution of a large number of compounds . This procedure is complicated as it needs correction equations to calculate the solubility in the aqueous phase containing no dimethyl sulfoxide. Turbidimetry and nephelometry are also used but they are based on the precipitation of material and, due to the possibility of over-saturation, might be inaccurate . The “saturation shake-flask method” is also widespread but it is time consuming [9, 10, 11, 12, 13, 14, 15, 16, 17]. The “generator column” method highly facilitates the reaching of solubility equilibrium  as the contacting area between the solid material and solvent is very high and the saturation concentration can be measured as usual in chromatography.
Solvent mixtures are used for analytical procedures like in determination of polycyclic aromatic hydrocarbons in oil extracts . There are a large number of works studying the effect of changing the molar ratio of the two and sometimes more solvents on the behavior of a compound [20, 21, 22]. There is a solubility determination method that uses a co-solvent introduced into the studied solution  but needs a complicated evaluation procedure.
The disadvantage of some methods is the need for a large quantity of solvents and solute, and on the other hand they are sometimes time consuming. The basic problem is usually that there is not a good reference for the exact determination of the unknown saturation concentration. When spectroscopic methods are applied, both the peak position and intensity are affected by the molecular environment of the solute molecules. With the electrochemical methods (mainly voltammetric and amperometric techniques) the composition of the solvent strongly influences the viscosity and consequently the diffusion coefficient that contributes significantly to the signal’s magnitude.
In this work, a reliable new method (co-solvent calibration method) is described which is based on ensuring the uniformity of the factors discussed above during the whole procedure.
The chemicals and solvents used were analytical grade (99%). The supporting electrolyte used in the cyclic voltammetric measurements was tetrabutylammonium perchlorate (TBuClO4) with 98% purity. Water contents of solvents were around 10−4 mmol·L−1. The working electrode was a platinum disc (1 mm in diameter) sealed in polyetheretherketone, the counter electrode was a platinum rod and a silver wire served as reference. The electrochemical measurements were carried out with a potentiostat (Dropsens, Spain).
All solutions were prepared in 10 cm3 glass flasks and thermostated at 298 K. The saturated solutions were prepared by adding hydroquinone in excess to the selected solvents. They were thoroughly mixed and ultrasonicated for approximately 5 min to facilitate the dissolution. Then, the solutions were allowed to sediment but the solid particles did not settle down completely. Due to the presence of solid particles, over-saturation can be avoided. The solid particles had to be separated from the saturated solutions so they were filtrated through a funnel (Sartorius 3hw, average pore size 8–12 μm). Before all measurements the funnel was thoroughly washed, firstly with distilled water and then with acetone to remove the contaminants and the traces of hydroquinone remained from the previous filtration. After complete evaporation of acetone a new filtration paper was placed into the funnel and the saturated solutions obtained after the filtration were used for the solubility determination.
Another important step was the washing of the solid hydroquinone to separate the microparticles which can go through the pores of filtration paper. The solid material, which was used in the experiments, consisted mainly of macrosized particles. This sample was thoroughly washed with carbon tetrachloride in the filtration paper so microparticles were removed which otherwise could cause error in the determination by penetrating its pores. This fraction was used therefore throughout the work.
When determination of the solubility of a selected material is important, the use of a co-solvent by the calibration method offers many advantages. The basic requirement for the co-solvent is that it has to be able to dissolve the solute in high concentration and be miscible with the solvent which is in focus of our interest (primary solvent). In electrochemistry, the permittivity of the co-solvent has to be high enough to dissolve the supporting electrolyte which is necessary to avoid the significant ohmic distortion. During the whole procedure the solvents from the same container should be used as the baseline or background current according to the chosen method, which otherwise could change in the case of different sources increasing the inaccuracy of determination. As the presence of contaminants can modify the solubility of the solute, the solute and primary solvent should have high purity.
Prepare a stock solution of the solute with the co-solvent whose concentration are adjusted for the preparation of the calibration solution series.
Add the supplementary materials (supporting electrolyte, indicator, metal salt, etc.) to the flasks in the necessary quantity.
Pipet the exact volume of the primary solvent into all flasks which are consistent with all calibration solutions.
Add the exact volumes of the stock solution of solute prepared with the co-solvent to set accurately the desired concentrations of calibration solutions, mix them thoroughly and fill the flasks to the marks with the pure co-solvent.
Pipet the same volume of saturated primary solvent to an additional empty flask containing the necessary supplementary materials in the same quantity as for the calibration solutions.
Fill the flask containing the saturated primary solvent also with the pure co-solvent to the mark with thorough mixing.
These steps ensure that the solute is at a well-defined concentration in the flasks as the solution volumes are set exactly. The errors caused by the volume changes due to the mixing of the two solvents can be minimized by adding the co-solvent in small portions with mixing. This procedure is applicable by a series of commonly used methods in analytical chemistry (electrochemical, spectroscopic, chromatographic, etc.).
The solubility (S) of the selected material can be calculated by using the next equation:
3 Results and Discussion
In order to determine the solubility of hydroquinone in the three selected chlorinated hydrocarbon solvents the proposed co-solvent calibration method was tested. Hydroquinone is a good model redox-active compound as it undergoes two electron oxidation producing protonated 1,4-benzoquinone . Therefore, electrochemical techniques are suitable to study this compound, so cyclic voltammetry was selected here, which is based on the recording of current peaks when macroelectrodes are used. Acetonitrile was chosen as the co-solvent as it is miscible with all chlorinated hydrocarbon solvents. It has a wide potential window in the positive range making it attractive for electrooxidation studies. On the other hand, it has a relatively high permittivity (ε = 36) which is an essential factor for the electrochemical measurements. The chlorinated hydrocarbons have low dielectric constants: 8.93 for dichloromethane, 4.8069 for trichloromethane and 2.2379 for carbon tetrachloride . The volumes of solvents were 50–50% of the total value for all chlorinated hydrocarbons. Therefore, the permittivities of mixtures were around 20, which is highly suitable for electrochemical measurements with macroelectrodes. Reliable voltammetric and amperometric measurements can be performed in liquids with permittivity higher than 5.
As nothing is known about the saturation concentration it must be estimated previously to fit the concentration range of calibration solutions to it. One possibility for getting an approximate value for it is the taking a voltammogram in the saturated solution with microelectrode and then in a higher permittivity solvent with known concentration of analyte in the presence of supporting electrolyte. Knowing the viscosities of the two solvents the approximate concentration can be estimated. This procedure worked in the case of dichloromethane, therefore the calibration range was between 0 and 10 mmol·L−1 In the case of trichloromethane well defined voltammogram could not be recorded due to the significant ion association of the supporting electrolyte and in the case of carbon tetrachloride the TBuClO4 did not dissolve at all. Therefore their mixtures prepared with acetonitrile were used and lower concentrations could be estimated. For the two last solvents the calibration range was set between 0 and 2.5 mmol·L−1.
A new co-solvent calibration method is introduced which is suitable for determination of the concentrations of both solid and liquid materials in a solvent. Furthermore, the method can gain applicability by its procedure, which uses small solution volumes. However, the dilution of samples can be disadvantageous because of the detection limit of selected method, reliable solubility values can be determined. Our plan for the future is the determination of solubilities of selected solid and liquid materials in aqueous and nonaqueous solvents by using the procedure introduced here. This is a promising method, also when the pH profile of solubility is in focus. It might be applicable by micro-plate techniques by knowing the correction factor for the volume change due to the mixing of two (or more) solvents. It can be used to determine the solubility not only in a solvent but also in solvent mixtures by using ternary or quaternary solvent mixtures for the determination.
Open access funding provided by University of Pé‚cs (PTE). Financial support of the GINOP 2.3.2-15-2016-00022 Grant are highly appreciated.
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