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The Protein Journal

, Volume 37, Issue 4, pp 333–352 | Cite as

Variability of Some Milk-Associated Genes and Proteins in Several Breeds of Saudi Arabian Camels

  • Elrashdy M. Redwan
  • Salah Korim
  • Amro Samra
  • Yasser Saad
  • Hussein A. Amhedar
  • Vladimir N. Uversky
Article

Abstract

To gain knowledge on the molecular basis of diversity of several clans of Saudi camel (Camelus dromedarius) characterization of these animals was conducted at both genetic and protein levels. To this end, blood and milk samples were collected from several camel breeds at different Saudi Arabia locations (northern Jeddah, Riyadh, and Alwagh governorates). Genomic DNA was extracted from blood of four Saudi camel breeds (Majahem, Safra, Wadha, and Hamara), and DNA fragments of the casein and α-lactalbumin genes were amplified. The retrieved DNA sequences were analyzed for genetic variability. The inter-simple sequence repeat technique was used for confirming the relationships among the analyzed camel breeds, and the PCR–RFLP with two restriction enzymes was utilized for exploring their molecular variations. The number of haplotypes, gene diversity, nucleotide diversity, average number of nucleotide differences, and sequence conservation were calculated for all the analyzed DNA sequences. These analyses revealed the presence of several single nucleotide polymorphisms in the analyzed DNA sequences. A group of neighbor joining trees was built for inferring the evolutionary variations among the studied animals. Protein profiling of milk from different camel clans was also conducted, and differences between and within the Saudi camel clans were easily found based on the isoelectric focusing (IEF) profiles using ampholytes with different IEF range. This study revealed that analyzed camel breeds show low levels of genetic differences. This may be a reflection of the evolutionary history of C. dromedarius that was domesticated based on a highly homogeneous ancestor ecotype.

Keywords

Camel Milk Variability Camel breeds alpha-lactalbumin casein polymorphism 

Notes

Funding

This work was funded by the King Abdulaziz City for Science and Technology General Directorate of Research Grants Programs under the Grant No. (A.B-35-195).

Compliance with Ethical Standards

Conflict of interest

Authors Elrashdy M. Redwan, Salah Korim, Amro Samra, Yasser Saad, Hussein A. Amhedar and Vladimir N. Uversky declares that they have no conflict of interest.

Research Involving Human Participants

This article does not contain any studies with human participants performed by any of the authors.

Research Involving Human Participants

This article does not contain any studies with human participants performed by any of the authors.

Supplementary material

10930_2018_9782_MOESM1_ESM.pdf (1.3 mb)
Supplementary material 1 (PDF 1292 KB)

References

  1. 1.
    Banaja AA, Ghandour AM (1994) A review of parasites of camels (Camelus dromedaries) in Saudi Arabia. J King Abdulaziz Univ 6:75–86CrossRefGoogle Scholar
  2. 2.
    Bekele T, Zeleke M, Baars RMT (2002) Milk production performance of one humped camel (Camelus dromedarius) under pastoral management in semi-arid eastern Ethiopia. Livest Prod Sci 76:37–44CrossRefGoogle Scholar
  3. 3.
    Zhang H, Yao J, Zhao D, Liu H, Li J, Guo M (2005) Changes in chemical composition of Alxa bactrian camel milk during lactation. J Dairy Sci 88:3402–3410CrossRefPubMedGoogle Scholar
  4. 4.
    Abdallah HR, Faye B (2012) Phenotypic classification of Saudi Arabian camel (Camelus dromedarius) by their body measurements. Emir J Food Agric 24:272–280Google Scholar
  5. 5.
    El Agamy EI (2006) Camel milk. In: Park YW, Haenlein FW (eds) Handbook of non-bovine mammals. Blackwell Publisher, Oxford, pp 297–344CrossRefGoogle Scholar
  6. 6.
    Shuiep ES, El-Zubeir IEM, Yousif IA, Erhardt G (2009) Genetic diversity among Sudanese indigenous camel (Camelus dromedarius) at milk protein genes, Vortragstagung der DGfZ und GfT am 16./17.At GieβenGoogle Scholar
  7. 7.
    Nikkah A (2011) Equidae, camel, and yak milks as functional foods: a review. J Nutr Food Sci 1:116Google Scholar
  8. 8.
    Kappeler S, Farah Z, Puhan Z (1998) Sequence analysis of Camelus dromedarius milk caseins. J Dairy Res 65:209–222CrossRefPubMedGoogle Scholar
  9. 9.
    Farah Z, Kappeler S, Mertz L, Brentse A (2004) Milk products. In: Farah Z, Fischer A (eds) Milk and meat from the camel: handbook on products and processing. VDF Hochschulverlag AG, ZürichGoogle Scholar
  10. 10.
    Farah Z, Mollet M, Younan M, Dahir R (2007) Camel dairy in Somalia: limiting factors and development potential. Livest Sci 110:187–191CrossRefGoogle Scholar
  11. 11.
    Redwan EM, Tabll A (2007) Camel lactoferrin markedly inhibits hepatitis C virus genotype 4 infection of human peripheral blood leukocytes. J Immunoass Immunochem 28:267–277CrossRefGoogle Scholar
  12. 12.
    Conesa C, Sanchez L, Rota C, Perez MD, Calvo M, Farnaud S, Evans RW (2008) Isolation of lactoferrin from milk of different species: calorimetric and antimicrobial studies. Comp Biochem Physiol B 150:131–139CrossRefPubMedGoogle Scholar
  13. 13.
    Redwan EM, El-Fakharany EM, Uversky VN, Linjawi MH (2014) Screening the anti infectivity potentials of native N- and C-lobes derived from the camel lactoferrin against hepatitis C virus. BMC Complement Altern Med 14:219CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Redwan EM, El-Baky NA, Al-Hejin AM, Baeshen MN, Almehdar HA, Elsaway A, Gomaa AB, Al-Masaudi SB, Al-Fassi FA, AbuZeid IE, Uversky VN (2016) Significant antibacterial activity and synergistic effects of camel lactoferrin with antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). Res Microbiol 167:480–491CrossRefPubMedGoogle Scholar
  15. 15.
    Albar AH, Almehdar HA, Uversky VN, Redwan EM (2014) Structural heterogeneity and multifunctionality of lactoferrin. Curr Protein Pept Sci 15:778–797CrossRefPubMedGoogle Scholar
  16. 16.
    Albar AH, El-Fakharany EM, Almehdar HA, Uversky VN, Redwan EM (2017) In vitro exploration of the anti-HCV potential of the synthetic spacer peptides derived from human, bovine, and camel lactoferrins. Protein Pept Lett 24:909–921PubMedGoogle Scholar
  17. 17.
    El-Fakharany EM, Uversky VN, Redwan EM (2017) comparative analysis of the antiviral activity of camel, bovine, and human lactoperoxidases against herpes simplex virus type 1. Appl Biochem Biotechnol 182:294–310CrossRefPubMedGoogle Scholar
  18. 18.
    El-Fakharany EM, El-Baky NA, Linjawi MH, Aljaddawi AA, Saleem TH, Nassar AY, Osman A, Redwan EM (2017) Influence of camel milk on the hepatitis C virus burden of infected patients. Exp Ther Med 13:1313–1320CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Ehlayel M, Bener A, Abu Hazeima K, Al-Mesaifri F (2011) Camel milk is a safer choice than goat milk for feeding children with cow milk allergy. ISRN Allergy 2011.  https://doi.org/10.5402/2011/391641 CrossRefGoogle Scholar
  20. 20.
    Cardoso RR, Santos RM, Cardoso CR, Carvalho MO (2010) Consumption of camel’s milk by patients intolerant to lactose. A preliminary study. Rev Alerg Mex 57:26–32PubMedGoogle Scholar
  21. 21.
    Cardoso RR, Ponte M, Leite V (2013) Protective action of camel milk in mice inoculated with Salmonella enterica. Isr Med Assoc J 15:5–8PubMedGoogle Scholar
  22. 22.
    Al-Hammadi S, El-Hassan T, Al-Reyami L (2010) Anaphylaxis to camel milk in an atopic child. Allergy 65:1623–1625CrossRefPubMedGoogle Scholar
  23. 23.
    Agrawal RP, Budania S, Sharma P, Gupta R, Kochar DK, Panwar RB, Sahani MS (2007) Zero prevalence of diabetes in camel milk consuming Raica community of north-west Rajasthan, India. Diab Res Clin Pract 76:290–296CrossRefGoogle Scholar
  24. 24.
    Agrawal RP, Dogra R, Mohta N, Tiwari R, Singhal S, Sultania S (2009) Beneficial effect of camel milk in diabetic nephropathy. Acta Biomed 80:131–134PubMedGoogle Scholar
  25. 25.
    Mohamad RH, Zekry ZK, Al-Mehdar HA, Salama O, El-Shaieb SE, El-Basmy AA, Al-said MG, Sharawy SM (2009) Camel milk as an adjuvant therapy for the treatment of type 1 diabetes: verification of a traditional ethnomedical practice. J Med Food 12:461–465CrossRefPubMedGoogle Scholar
  26. 26.
    Agrawal RP, Tantia P, Jain S, Agrawal R, Agrawal V (2013) Camel milk: a possible boon for type 1 diabetic patients. Cell Mol Biol 59:99–107PubMedGoogle Scholar
  27. 27.
    Zibaee S, Hosseini SM, Yousefi M, Taghipour A, Kiani MA, Noras MR (2015) Nutritional and therapeutic characteristics of camel milk in children: a systematic review. Electron Physician 7:1523–1528CrossRefPubMedPubMedCentralGoogle Scholar
  28. 28.
    Mohanty DP, Mohapatra S, Misra S, Sahu PS (2016) Milk derived bioactive peptides and their impact on human health—a review. Saudi J Biol Sci 23:577–583CrossRefPubMedGoogle Scholar
  29. 29.
    Cekici H, Sanlier N (2017) Current nutritional approaches in managing autism spectrum disorder: a review. Nutr Neurosci.  https://doi.org/10.1080/1028415X.2017.1358481 CrossRefPubMedGoogle Scholar
  30. 30.
    Mirmiran P, Ejtahed HS, Angoorani P, Eslami F, Azizi F (2017) Camel milk has beneficial effects on diabetes mellitus: a systematic review. Int J Endocrinol Metab 15:e42150PubMedPubMedCentralGoogle Scholar
  31. 31.
    Almathen F, Mwaracharo J, Hanotte O (2012) Genetic diversity and relationships of indigenous Saudi Arabia camel Camelus dromedarius populations. In: Johnson EH, Mahgoub O, Eljack AH, Kadim IT, Bobade PA, Tageldin MH, Al-Marzooqi WS, El Tahir Ahmed Y (eds) 3rd Conference of the International Society of Camelid Research and Development (ISOCARD), Mascate (Sultanate of Oman), pp 40–41Google Scholar
  32. 32.
    Crawford AM, Littlejohn RP (1998) The use of DNA markers in deciding conservation priorities in sheep and other livestock. Anim Genet Resour Inf 23:21–26CrossRefGoogle Scholar
  33. 33.
    El-Hanafy AA, El-Saadani MA (2009) Fingerprinting of FecB gene in five Egyptian sheep breeds. Biotechnol Anim Husb 25:205–212CrossRefGoogle Scholar
  34. 34.
    EL-Hanafy AA, Salem HH (2009) PCR-RFLP of IGFBP-3 gene in some Egyptian sheep breeds. Am-Eurasian J Agric Environ Sci 5:82–85Google Scholar
  35. 35.
    Kumar S, Gupta J, Kumar N, Dikshit K, Navani N, Jain P, Nagarajan M (2006) Genetic variation and relationships among eight Indian riverine buffalo breeds. Mol Ecol 15:593–600CrossRefPubMedGoogle Scholar
  36. 36.
    El-Hanafy AA, El-Saadani MA, Eissa M, Maharem GM, Khalifa ZA (2010) Polymorphism of β-lactoglobulin gene in Barki and Damascus and their crossbred goats in relation to milk yield. Biotechnol Anim Husb 26:1–12CrossRefGoogle Scholar
  37. 37.
    El-Hanafy AA, Sabir JSM, Mutawakil MHZ, Elsoud MEA, Abdel-Sadek MA (2012) Polymorphism of β-lactoglobulin gene in Barki sheep breed. Biotechnol Anim Husb 28:231–239CrossRefGoogle Scholar
  38. 38.
    Sabir JSM, Mutawakil MHZ, El-Hanafy AA, Ahmed MM (2012) Genetics similarity among four breeds of goat in Saudi Arabia detected by random amplified polymorphic DNA marker. Afr J Biotechnol 11:3958–3963CrossRefGoogle Scholar
  39. 39.
    Erhardt G (1996) Detection of a new kappa-casein variant in milk of Pinzgauer cattle. Anim Genet 27:105–107CrossRefPubMedGoogle Scholar
  40. 40.
    Caroli AM, Chessa S, Erhardt GJ (2009) Invited review: milk protein polymorphisms in cattle: effect on animal breeding and human nutrition. J Dairy Sci 92:5335–5352CrossRefPubMedGoogle Scholar
  41. 41.
    Caroli A, Chiatti F, Chessa S, Rignanese D, Ibeagha-Awemu EM, Erhardt G (2007) Characterization of the casein gene complex in West African goats and description of a new alpha(s1)-casein polymorphism. J Dairy Sci 90:2989–2996CrossRefPubMedGoogle Scholar
  42. 42.
    Moioli B, Pilla F, Tripaldi C (2008) Detection of milk protein genetic polymorphisms in order to improve dairy traits in sheep and goats: a review. Small Rumin Res 27:185–195CrossRefGoogle Scholar
  43. 43.
    Shuiep ES, Giambra IJ, El-Zubeir IEM, Erhardt G (2013) Biochemical and molecular characterization of polymorphisms of αs1-casein in Sudanese camel (Camelus dromedarius) milk. Int Dairy J 28:88–93CrossRefGoogle Scholar
  44. 44.
    Ochirkhuyag B, Chobert JM, Dalgalarrondo M, Choiset Y, Haertle T (1998) Characterization of whey proteins from Mongolian yak, Khainak, and Bacterian camel. J Food Biochem 22:105–124CrossRefGoogle Scholar
  45. 45.
    Conti A, Godovac-Zimmermann J, Napoletano L, Liberatori J (1985) Identification and characterization of two α-lactalbumins from Somali camel milk (Camelus dromedarius). Milchwissenschaft 40:673–675Google Scholar
  46. 46.
    Farah Z (1993) Composition and characteristics of camel milk. J Dairy Res 60:603–626CrossRefPubMedGoogle Scholar
  47. 47.
    Almahdy O, El-Fakharany EM, El-Dabaa E, Ng TB, Redwan EM (2011) Examination of the activity of camel milk casein against hepatitis C virus (genotype-4a) and its apoptotic potential in hepatoma and hela cell lines. Hepat Mon 11:724–730CrossRefPubMedPubMedCentralGoogle Scholar
  48. 48.
    Pettersson J, Mossberg AK, Svanborg C (2006) alpha-Lactalbumin species variation, HAMLET formation, and tumor cell death. Biochem Biophys Res Commun 345:260–270CrossRefPubMedGoogle Scholar
  49. 49.
    Redwan EM (2012) Simple, sensitive, and quick protocol to detect less than 1 ng of bacterial lipopolysaccharide. Prep Biochem Biotechnol 42:171–182CrossRefPubMedGoogle Scholar
  50. 50.
    Erhardt G (1989) Evidence for a third allele at the beta-lactoglobulin (beta-Lg) locus of sheep milk and its occurrence in different breeds. Anim Genet 20:197–204CrossRefPubMedGoogle Scholar
  51. 51.
    Erhardt G, Shuiep el TS, Lisson M, Weimann C, Wang Z, El Zubeir Iel Y, Pauciullo A (2016) Alpha S1-casein polymorphisms in camel (Camelus dromedarius) and descriptions of biological active peptides and allergenic epitopes. Trop Anim Health Prod 48:879–887CrossRefPubMedGoogle Scholar
  52. 52.
    Pauciullo A, Shuiep ES, Cosenza G, Ramunno L, Erhardt G (2013) Molecular characterization and genetic variability at kappa-casein gene (CSN3) in camels. Gene 513:22–30CrossRefPubMedGoogle Scholar
  53. 53.
    Pauciullo A, Giambra IJ, Iannuzzi L, Erhardt G (2014) The beta-casein in camels: molecular characterization of the CSN2 gene, promoter analysis and genetic variability. Gene 547:159–168CrossRefPubMedGoogle Scholar
  54. 54.
    Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefPubMedPubMedCentralGoogle Scholar
  55. 55.
    Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452CrossRefPubMedGoogle Scholar
  56. 56.
    Yeh FC, Boyle TJB (1997) Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belg J Bot 129:157Google Scholar
  57. 57.
    Shah MG, Reissmann M, Qureshi AS, Schwartz HJ (2008) Evaluation of six camel breeds for heterozygocity through restriction fragment length polymorphism. Pak Vet J 28:13–16Google Scholar
  58. 58.
    El-Hanafy AA, Saad YM, Alkarim SA, Almehdar HA, Aalzahrani F, Almatry MA, Uversky VN, Redwan EM (2018) Seasonal variability in the yield and composition of milk from several breeds of Saudi camels. AnimalsGoogle Scholar
  59. 59.
    Mohamed A (1993) Characterization of camel milk beta-casein. University of Karachi, PakistanGoogle Scholar
  60. 60.
    Farah Z (1996) Camel milk properties and products. Swiss Centre for Developments Cooperation in Technology and Management, St. GallenGoogle Scholar
  61. 61.
    Restani P, Gaiaschi A, Plebani A, Beretta B, Cavagni G, Fiocchi A, Poiesi C, Velona T, Ugazio AG, Galli CL (1999) Cross-reactivity between milk proteins from different animal species. Clin Exp Allergy 29:997–1004CrossRefPubMedGoogle Scholar
  62. 62.
    Abdulrahman AO, Ismael MA, Al-Hosaini K, Rame C, Al-Senaidy AM, Dupont J, Ayoub MA (2016) Differential effects of camel milk on insulin receptor signaling—toward understanding the insulin-like properties of camel milk. Front Endocrinol 7:4CrossRefGoogle Scholar
  63. 63.
    Salmen SH, Abu-Tarboush HM, Al-Saleh AA, Metwalli AA (2012) Amino acids content and electrophoretic profile of camel milk casein from different camel breeds in Saudi Arabia. Saudi J Biol Sci 19:177–183CrossRefPubMedGoogle Scholar
  64. 64.
    Al Haj OA, Kanhal HA (2010) Compositional, technological and nutritional aspects of dromedary camel milk. Int Dairy J 20:811–821CrossRefGoogle Scholar
  65. 65.
    El-Agamy EI (2006) Camel milk. In: Park YW, Haenlein FW (eds) Handbook of non-bovine mammals. Blackwell, Ames, pp 297–344CrossRefGoogle Scholar
  66. 66.
    Wakabayashi H, Yamauchi K, Takase A (2006) Lactoferrin research, technology and applications. Int Dairy J 16:1241–1251CrossRefGoogle Scholar
  67. 67.
    Cohen MS, Britigan BE, French M, Bean K (1987) Preliminary observations on lactoferrin secretion in human vaginal mucus: variation during the menstrual cycle, evidence of hormonal regulation, and implications for infection with Neisseria gonorrhoeae. Am J Obstet Gynecol 157:1122–1125CrossRefPubMedGoogle Scholar
  68. 68.
    Harmsen MC, Swart PJ, de Bethune MP, Pauwels R, De Clercq E, The TH, Meijer DK (1995) Antiviral effects of plasma and milk proteins: lactoferrin shows potent activity against both human immunodeficiency virus and human cytomegalovirus replication in vitro. J Infect Dis 172:380–388CrossRefPubMedGoogle Scholar
  69. 69.
    Farnaud S, Evans RW (2003) Lactoferrin-a multifunctional protein with antimicrobial properties. Mol Immunol 40:395–405CrossRefPubMedGoogle Scholar
  70. 70.
    Sanchez L, Calvo M, Brock JH (1992) Biological role of lactoferrin. Arch Dis Child 67:657–661CrossRefPubMedPubMedCentralGoogle Scholar
  71. 71.
    Ng ML, Lee JW, Leong ML, Ling AE, Tan HC, Ooi EE (2004) Topographic changes in SARS coronavirus-infected cells at late stages of infection. Emerg Infect Dis 10:1907–1914CrossRefPubMedPubMedCentralGoogle Scholar
  72. 72.
    El-Fakharany EM, Abedelbaky N, Haroun BM, Sanchez L, Redwan NA, Redwan EM (2012) Anti-infectivity of camel polyclonal antibodies against hepatitis C virus in Huh7.5 hepatoma. Virol J 9:201CrossRefPubMedPubMedCentralGoogle Scholar
  73. 73.
    Liao Y, El-Fakkarany E, Lonnerdal B, Redwan EM (2012) Inhibitory effects of native and recombinant full-length camel lactoferrin and its N and C lobes on hepatitis C virus infection of Huh7.5 cells. J Med Microbiol 61:375–383CrossRefPubMedGoogle Scholar
  74. 74.
    Bethell DR, Huang J (2004) Recombinant human lactoferrin treatment for global health issues: iron deficiency and acute diarrhea. Biometals 17:337–342CrossRefPubMedGoogle Scholar
  75. 75.
    El-Fakharany EM, Sanchez L, Al-Mehdar HA, Redwan EM (2013) Effectiveness of human, camel, bovine and sheep lactoferrin on the hepatitis C virus cellular infectivity: comparison study. Virol J 10:199CrossRefPubMedPubMedCentralGoogle Scholar
  76. 76.
    Smith LM, Kelleher NL, P. Consortium for Top Down (2013) Proteoform: a single term describing protein complexity. Nat Methods 10:186–187CrossRefPubMedPubMedCentralGoogle Scholar
  77. 77.
    Uversky VN (2016) p53 Proteoforms and intrinsic disorder: an illustration of the protein structure-function continuum concept. Int J Mol Sci 17:1874CrossRefPubMedCentralGoogle Scholar
  78. 78.
    Beadle GW, Tatum EL (1941) Genetic control of biochemical reactions in Neurospora. Proc Natl Acad Sci USA 27:499–506CrossRefPubMedGoogle Scholar
  79. 79.
    Schluter H, Apweiler R, Holzhutter HG, Jungblut PR (2009) Finding one’s way in proteomics: a protein species nomenclature. Chem Cent J 3:11CrossRefPubMedPubMedCentralGoogle Scholar
  80. 80.
    Mahmoud AH, Alshaikh MA, Aljumaah RS, Mohammed OB (2012) Genetic variability of camel (Camelus dromedarius) populations in Saudi Arabia based on microsatellites analysis. Afr J Biotechnol 11:11173–11180CrossRefGoogle Scholar
  81. 81.
    Mahrous KF, Ramadan HAI, Abdel-Aziem SH, Abd-El M, Mordy DM, Hemdan (2011) Genetic variations between camel breeds using microsatellite markers and RAPD techniques. J Appl Biosci 39:2626–2634Google Scholar
  82. 82.
    Saad YM, El-Hanafy AA, Alkarim SA, Almehdar HA, Redwan EM (2017) Analysis of genetic variations in some camels (Camelus dromedarius) breeds. In: 19th International conference on animal production, mating and breeding (ICAPMB), IstanbulGoogle Scholar
  83. 83.
    Shah MG, Qureshi AS, Reissmann M, Schwartz HJ (2006) Sequencing and sequence analysis of myostatin gene in the exon 1 of the Camel (Camelus dromedarius). Pak Vet J 26:176–178Google Scholar
  84. 84.
    Vaccarelli G, Antonacci R, Tasco G, Yang F, Giordano L, El Ashmaoui HM, Hassanane MS, Massari S, Casadio R, Ciccarese S (2012) Generation of diversity by somatic mutation in the Camelus dromedarius T-cell receptor gamma variable domains. Eur J Immunol 42:3416–3428CrossRefPubMedGoogle Scholar
  85. 85.
    Prasad S, Ali SA, Banerjee P, Joshi J, Sharma U, Vijh RK (2014) Identification of SNPs and their validation in camel (Camelus bactrianus and Camelus dromedarius). J Agric Vet Sci 7:65–70Google Scholar
  86. 86.
    Frankham R, Ballou JD, Briscoe DA (2009) Introducion to conservation genetics, 2nd edn. Cambridge University Press, CambridgeGoogle Scholar
  87. 87.
    Saad YM, AbuZinadah OAH, El-Domyati FM, Sabir JM (2012) Analysis of genetic signature for some Plectropomus species based on some dominant DNA markers. Life Sci J 9:2370–2375Google Scholar
  88. 88.
    El-Hanafy AA, Saad YM, Alkarim SA, Almehdar HA, Redwan EM (2016) Camel genetic resources conservation in Saudi Arabia via molecular markers. Wulfenia J 23:88–103Google Scholar
  89. 89.
    Hoffmann I (2010) Climate change and the characterization, breeding and conservation of animal genetic resources. Anim Genet 41(Suppl 1):32–46CrossRefPubMedGoogle Scholar
  90. 90.
    Faye B (2015) Special issue on “Some preliminary results of the PROCAMED project (promotion of innovations in the camel sector for a sustainable development in the Mediterranean basin). Emir J Food Agric 27:1–4CrossRefGoogle Scholar
  91. 91.
    Al-Hazmi MA, Ghandour AM, Gohar MA (1994) Study of the biometry of some breeds of Arabian camel (Camelus dromedarius) in Saudi Arabia. J King Abdulaziz Univ Sci 6:87–99CrossRefGoogle Scholar
  92. 92.
    Saad YM, El-Shikh OAM (2015) Analysis of molecular variations in some Sox14 gene fragments in some ray-finned fishes. Wulfenia J 22:80–92Google Scholar
  93. 93.
    Mehta SC, Mishra BP, Sahani MS (2006) Genetic differentiation of Indian camel (Camelus dromedarius) breeds using random oligonucleotide primers. AGRI 39:77–88Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Elrashdy M. Redwan
    • 1
    • 2
  • Salah Korim
    • 1
  • Amro Samra
    • 1
  • Yasser Saad
    • 1
  • Hussein A. Amhedar
    • 1
  • Vladimir N. Uversky
    • 1
    • 3
    • 4
  1. 1.Biology Department, Faculty of ScienceKing Abdulaziz UniversityJeddahSaudi Arabia
  2. 2.Therapeutic and Protective Proteins Laboratory, Protein Research Department, Genetic Engineering and Biotechnology Research InstituteCity for Scientific Research and Technology ApplicationsNew Borg EL-ArabEgypt
  3. 3.Institute for Biological Instrumentation of the Russian Academy of SciencesPushchinoRussia
  4. 4.Department of Molecular Medicine and USF Health Byrd Alzheimer’s Research Institute, Morsani College of MedicineUniversity of South FloridaTampaUSA

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