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Trans-sialidase Protein as a Potential Serological Marker for African Trypanosomiasis

  • Ana Filipa Teixeira
  • João Gomes Pereira
  • Sónia Pestana-Ascensão
  • Marcelo Sousa SilvaEmail author
Article

Abstract

Trypanosoma brucei is the etiological agent of African trypanosomiasis responsible for human and animal infections. T. brucei is transmitted by infected tsetse flies. There is no vaccine for the disease and drugs available for treatment are inefficient and high toxicity. In this context, it is a priority to find antigenic targets suitable for the development of new diagnostic tools, drugs and vaccines. In this work, we report that mice infected with T. b. brucei produce antibodies against trans-sialidase recombinant protein (TS). In addition, we also demonstrate that bloodstream T. b. brucei express messenger RNA related to the TS gene. Collectively, our data strongly suggest that bloodstream forms of T. b. brucei also express the TS gene, that to date was described only in the procyclic forms of the T. b. brucei. In conclusion, these results highlight the importance of TS protein as a possible antigen target during infection caused by T. b. brucei.

Keywords

Trans-sialidase Trypanosoma brucei African trypanosomiasis Antigenic targets Serological markers 

Notes

Acknowledgements

Thanks to Global Health and Tropical Medicine (GHTM-UID/multi/04413/2013) by support and the Programa Ciências Sem Fronteiras, Capes—Brazil (Grant No. 019/2013). We are also grateful to Paulo Fanado for editing this manuscript.

Author Contributions

Conceived and designed the experiments: AFT and MSS; Performed the experiments: AFT, JGP, and SPA; Analysed the data: AFT, JGP, SPA, and MSS; Wrote the paper: AFT and MSS. All authors read and approved the final version of the manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest.

Ethics Approval

Murine model was performed in accordance with existing legislation in Portugal and in accordance of the Ethical Committee at Institute of Hygiene and Tropical Medicine, Lisbon—Portugal.

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Global Health and Tropical Medicine, Institute of Hygiene and Tropical MedicineUniversidade Nova de LisboaLisbonPortugal
  2. 2.Immunoparasitology Laboratory, Department of Clinical and Toxicological AnalysisFederal University of Rio Grande do NorteNatalBrazil
  3. 3.Programa de Pós-graduação em Bioquímica, Centro de BiociênciasFederal University of Rio Grande do NorteNatalBrazil
  4. 4.Programa de Pós-graduação em Ciências Farmacêuticas, Centro de Ciências da SaúdeFederal University of Rio Grande do NorteNatalBrazil

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