Journal of Polymers and the Environment

, Volume 27, Issue 12, pp 2819–2830 | Cite as

Preparation of Chitosan, Sodium Alginate, Gelatin and Collagen Biodegradable Sponge Composites and their Application in Wound Healing and Curcumin Delivery

  • Negar Naghshineh
  • Kambiz TahvildariEmail author
  • Maryam Nozari
Original Paper


This research aimed to produce three chitosan-based biodegradable sponge composites from collagen, gelatin, and sodium alginate and through addition of curcumin to investigate their biological effects on wound healing. To this end, the Chitosan-Collagen-Curcumin (Chs, Col, Cur), Chitosan-Gelatin-Curcumin (Chs, Gel, Cur), and Chitosan-Alginate-Curcumin (Chs, Alg, Cur) sponge composites were prepared and subject to FT-IR, SEM, TGA, water absorption, biodegradability, wound healing and anti-bacterial analyses. Based on the results, the highest and lowest water absorptions were associated with Chitosan-Alginate-Curcumin and Chitosan-Collagen-Curcumin composites, respectively. Moreover, according to the SEM images, the highest porosity and the largest cavity size were associated with the Chitosan-Alginate-Curcumin composite. The biodegradability analysis results revealed that Chitosan-Alginate-Curcumin was completely destroyed in 4 days, while the Chitosan-Collagen-Curcumin composite showed the lowest level of destruction. Moreover, the highest amount of curcumin was released by the Chitosan-Gelatin-Curcumin composite which happened during the first hour. Finally, the highest wound healing effect was achieved within a 10-day period using the Chitosan-Gelatin-Curcumin composite, completely healing the wound on the mouse skin. On the other hand, the lowest effect was associated with the Chitosan-Alginate-Curcumin composite. The antibacterial tests suggested that all composites exhibited anti-bacterial capabilities, the highest level of which was associated with the Chitosan-Gelatin-Curcumin composite. Moreover, based on the histological tests, the fastest tissue repair process with the highest quality was achieved using the Chitosan-Gelatin-Curcumin sponge composite.


Chitosan sponge Collagen Gelatin Curcumin Wound healing 



  1. 1.
    Maiti S (2014) Effects of medical grade chitosan powder with xenogenic mesenchymal stem cell for full thickness wound healing in rat model. Int J Vet Sci 3:129–134Google Scholar
  2. 2.
    Trinca RB, Westin CB, da Silva JAF, Moraes ÂM (2017) Electrospun multilayer chitosan scaffolds as potential wound dressings for skin lesions. Eur Polym J 88:161–170. CrossRefGoogle Scholar
  3. 3.
    Baghaie S, Khorasani MT, Zarrabi A, Moshtaghian J (2017) Wound healing properties of PVA/starch/chitosan hydrogel membranes with nano zinc oxide as antibacterial wound dressing material. J Biomater Sci Polym Ed 28:2220–2241. CrossRefPubMedGoogle Scholar
  4. 4.
    Suarato G, Bertorelli R, Athanassiou A (2018) Borrowing from nature: biopolymers and biocomposites as smart wound care materials. Front Bioeng Biotechnol 6:137. CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Limpisophon K, Tanaka M, Osako K (2010) Characterisation of gelatin–fatty acid emulsion films based on blue shark (Prionace glauca) skin gelatin. Food Chem 122:1095–1101. CrossRefGoogle Scholar
  6. 6.
    Miranda SP, Garnica O, Sagahon AVL, Cardenas G (2004) Water vapor permeability and mechanical properties of chitosan composite films. J Chil Chem Soc 49(2):173–178Google Scholar
  7. 7.
    Pereda M, Ponce AG, Marcovich NE et al (2011) Chitosan–gelatin composites and bi-layer films with potential antimicrobial activity. Food Hydrocoll 25:1372–1381. CrossRefGoogle Scholar
  8. 8.
    Pranoto Y, Lee CM, Park HJ (2007) Characterizations of fish gelatin films added with gellan and κ-carrageenan. LWT Food Sci Technol 40:766–774. CrossRefGoogle Scholar
  9. 9.
    Prystupa DA, Donald AM (1996) Infrared study of gelatin conformations in the gel and sol states. Polym Gels Netw 4:87–110. CrossRefGoogle Scholar
  10. 10.
    Saki J, Khodanazary A, Hosseini SM (2018) Effect of chitosan–gelatin composite and bi-layer coating combined with pomegranate peel extract on quality properties of Belanger’s Croaker (Johnius belangerii) stored in refrigerator. J Aquat Food Prod Technol 27:557–567. CrossRefGoogle Scholar
  11. 11.
    Sanandam M, Salunkhe A, Shejale K, Patil D (2013) Chitosan bandage for faster blood clotting and wound healing. Int J Adv Biotechnol Res 4(1):47–50Google Scholar
  12. 12.
    Han F, Dong Y, Su Z et al (2014) Preparation, characteristics and assessment of a novel gelatin–chitosan sponge scaffold as skin tissue engineering material. Int J Pharm 476:124–133. CrossRefPubMedGoogle Scholar
  13. 13.
    Wang W, Hao X, Chen S, Yang Z (2018) pH-responsive Capsaicin@chitosan nanocapsules for antibiofouling in marine applications. Polymer 158:223–230. CrossRefGoogle Scholar
  14. 14.
    Muyonga JH, Cole CGB, Duodu KG (2004) Fourier transform infrared (FTIR) spectroscopic study of acid soluble collagen and gelatin from skins and bones of young and adult Nile perch (Lates niloticus). Food Chem 86:325–332. CrossRefGoogle Scholar
  15. 15.
    Dai M, Zheng X, Xu X et al (2009) Chitosan-alginate sponge: preparation and application in curcumin delivery for dermal wound healing in rat. J BioMed Biotechnol 2009:595126CrossRefGoogle Scholar
  16. 16.
    Joshi JR, Patel RP (2012) Role of biodegradable polymers in drug delivery. Int J Curr Pharm Res 4:74–81.Google Scholar
  17. 17.
    Tian J, Shao Q, Zhao J et al (2019) Microwave solvothermal carboxymethyl chitosan templated synthesis of TiO2/ZrO2 composites toward enhanced photocatalytic degradation of Rhodamine B. J Colloid Interface Sci 541:18–29. CrossRefPubMedGoogle Scholar
  18. 18.
    Zhao B, Shao Q, Hao L et al (2018) Yeast-template synthesized Fe-doped cerium oxide hollow microspheres for visible photodegradation of acid orange 7. J Colloid Interface Sci 511:39–47. CrossRefPubMedGoogle Scholar
  19. 19.
    Pan D, Ge S, Zhang X et al (2018) Synthesis and photoelectrocatalytic activity of In2O3 hollow microspheres via a bio-template route using yeast templates. Dalton Trans 47:708–715. CrossRefPubMedGoogle Scholar
  20. 20.
    Shi Z, Xu G, Deng J, Dong M, Murugadoss V, Liu C, Shao Q, Wu S, Guo Z (2019) Structural characterization of lignin from D. sinicus by FTIR and NMR techniques. Green Chem Lett Rev 12(3):235–243. CrossRefGoogle Scholar
  21. 21.
    Xu G, Shi Z, Zhao Y et al (2019) Structural characterization of lignin and its carbohydrate complexes isolated from bamboo (Dendrocalamus sinicus). Int J Biol Macromol 126:376–384. CrossRefPubMedGoogle Scholar
  22. 22.
    Shi Z, Jia C, Wang D et al (2019) Synthesis and characterization of porous tree gum grafted copolymer derived from Prunus cerasifera gum polysaccharide. Int J Biol Macromol 133:964–970. CrossRefPubMedGoogle Scholar
  23. 23.
    Ma Y, Lv L, Guo Y et al (2017) Porous lignin based poly (acrylic acid)/organo-montmorillonite nanocomposites: swelling behaviors and rapid removal of Pb (II) ions. Polymer 128:12–23. CrossRefGoogle Scholar
  24. 24.
    Yang J, Yang W, Wang X, Dong M, Liu H, Wujcik EK, Shao Q, Wu S, Ding T, Guo Z (2019) Synergistically toughening polyoxymethylene by methyl methacrylate–butadiene–styrene copolymer and thermoplastic polyurethane. Macromol Chem Phys 220(12):1800567. CrossRefGoogle Scholar
  25. 25.
    Ma L, Li N, Wu G et al (2018) Interfacial enhancement of carbon fiber composites by growing TiO2 nanowires onto amine-based functionalized carbon fiber surface in supercritical water. Appl Surf Sci 433:560–567. CrossRefGoogle Scholar
  26. 26.
    Ma L, Zhu Y, Feng P et al (2019) Reinforcing carbon fiber epoxy composites with triazine derivatives functionalized graphene oxide modified sizing agent. Composites B 176:107078. CrossRefGoogle Scholar
  27. 27.
    He Y, Yang S, Liu H et al (2018) Reinforced carbon fiber laminates with oriented carbon nanotube epoxy nanocomposites: magnetic field assisted alignment and cryogenic temperature mechanical properties. J Colloid Interface Sci 517:40–51. CrossRefPubMedGoogle Scholar
  28. 28.
    Liu M, Li B, Zhou H et al (2017) Extraordinary rate capability achieved by a 3D “skeleton/skin” carbon aerogel–polyaniline hybrid with vertically aligned pores. Chem Commun 53:2810–2813. CrossRefGoogle Scholar
  29. 29.
    Liang T, Qi L, Ma Z et al (2019) Experimental study on thermal expansion coefficient of composite multi-layered flaky gun propellants. Composites B 166:428–435. CrossRefGoogle Scholar
  30. 30.
    Gu H, Xu X, Cai J et al (2019) Controllable organic magnetoresistance in polyaniline coated poly(p-phenylene-2,6-benzobisoxazole) short fibers. Chem Commun 55:10068–10071. CrossRefGoogle Scholar
  31. 31.
    Guo Z, Yang P, Yang L et al (2019) Anchoring carbon nanotubes and post-hydroxylation treatment enhanced Ni nanofiber catalysts towards efficient hydrous hydrazine decomposition for an effective hydrogen generation. Chem Commun 55:9011–9014. CrossRefGoogle Scholar
  32. 32.
    Berndt AJ, Hwang J, Islam MD et al (2019) Poly(sulfur-random-(1,3-diisopropenylbenzene)) based mid-wavelength infrared polarizer: optical property experimental and theoretical analysis. Polymer 176:118–126. CrossRefGoogle Scholar
  33. 33.
    Ma Y, Hou C, Zhang H et al (2019) Three-dimensional core-shell Fe3O4/polyaniline coaxial heterogeneous nanonets: preparation and high performance supercapacitor electrodes. Electrochim Acta 315:114–123. CrossRefGoogle Scholar
  34. 34.
    Wu Z, Cui H, Chen L et al (2018) Interfacially reinforced unsaturated polyester carbon fiber composites with a vinyl ester-carbon nanotubes sizing agent. Compos Sci Technol 164:195–203. CrossRefGoogle Scholar
  35. 35.
    Li Y, Zhang T, Jiang B et al (2019) Interfacially reinforced carbon fiber silicone resin via constructing functional nano-structural silver. Compos Sci Technol 181:107689. CrossRefGoogle Scholar
  36. 36.
    Wu Z, Gao S, Chen L, Jiang S, Shao Q, Zhang B, Zhai Z, Wang C, Zhao M, Ma Y, Zhang X, Weng L, Zhang M, Guo Z (2017) Electrically insulated epoxy nanocomposites reinforced with synergistic core–shell SiO2@MWCNTs and montmorillonite bifillers. Macromol Chem Phys 218(23):1700357. Accessed 16 Aug 2019CrossRefGoogle Scholar
  37. 37.
    Xu J, Li K, Deng H et al (2019) Preparation of MCA-SiO2 and its flame retardant effects on glass fiber reinforced polypropylene. Fibers Polym 20:120–128. CrossRefGoogle Scholar
  38. 38.
    Mano JF, Sousa RA, Boesel LF et al (2004) Bioinert, biodegradable and injectable polymeric matrix composites for hard tissue replacement: state of the art and recent developments. Compos Sci Technol 64:789–817. CrossRefGoogle Scholar
  39. 39.
    Versypt ANF, Pack DW, Braatz RD (2013) Mathematical modeling of drug delivery from autocatalytically degradable PLGA microspheres—a review. J Control Release 165:29–37. CrossRefGoogle Scholar
  40. 40.
    Ofokansi K, Winter G, Fricker G, Coester C (2010) Matrix-loaded biodegradable gelatin nanoparticles as new approach to improve drug loading and delivery. Eur J Pharm Biopharm 76:1–9. CrossRefPubMedGoogle Scholar
  41. 41.
    Xuan D, Zhou Y, Nie W, Chen P (2017) Sodium alginate-assisted exfoliation of MoS2 and its reinforcement in polymer nanocomposites. Carbohydr Polym 155:40–48. CrossRefPubMedGoogle Scholar
  42. 42.
    Okeke OC, Boateng JS (2017) Nicotine stabilization in composite sodium alginate based wafers and films for nicotine replacement therapy. Carbohydr Polym 155:78–88. CrossRefPubMedGoogle Scholar
  43. 43.
    Li Q-Q, Wang Y-S, Chen H-H et al (2017) Retardant effect of sodium alginate on the retrogradation properties of normal cornstarch and anti-retrogradation mechanism. Food Hydrocoll 69:1–9. CrossRefGoogle Scholar
  44. 44.
    Ma R, Wang Y, Qi H et al (2019) Nanocomposite sponges of sodium alginate/graphene oxide/polyvinyl alcohol as potential wound dressing: in vitro and in vivo evaluation. Composites B 167:396–405. CrossRefGoogle Scholar
  45. 45.
    Xie Y, Yi Z, Wang J et al (2018) Carboxymethyl konjac glucomannan—crosslinked chitosan sponges for wound dressing. Int J Biol Macromol 112:1225–1233. CrossRefPubMedGoogle Scholar
  46. 46.
    Nguyen VC, Nguyen VB, Hsieh M-F (2013) Curcumin-loaded chitosan/gelatin composite sponge for wound healing application. Int J Polym Sci 2013: Article ID 106570Google Scholar
  47. 47.
    Tangsadthakun C, Kanokpanont S, Sanchavanakit N, Banaprasert T (2006) Properties of collagen/chitosan scaffolds for skin tissue engineering. J Met Mater Miner 16(1):37–44Google Scholar
  48. 48.
    Sun H, Yang Z, Pu Y et al (2019) Zinc oxide/vanadium pentoxide heterostructures with enhanced day-night antibacterial activities. J Colloid Interface Sci 547:40–49. CrossRefPubMedGoogle Scholar
  49. 49.
    Yang Z, Hao X, Chen S et al (2019) Long-term antibacterial stable reduced graphene oxide nanocomposites loaded with cuprous oxide nanoparticles. J Colloid Interface Sci 533:13–23. CrossRefPubMedGoogle Scholar
  50. 50.
    Safaei M, Taran M, Imani MM (2019) Preparation, structural characterization, thermal properties and antifungal activity of alginate–CuO bionanocomposite. Mater Sci Eng C 101:323–329. CrossRefGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Islamic Azad UniversityTehranIran

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