Advertisement

Biosynthesis of P3HBV-b-P3HB-b-P3HBV Triblock Copolymer by Ralstonia eutropha

  • Takahiko NakaokiEmail author
  • Junya Yasui
  • Tomo Komaeda
Original paper
  • 10 Downloads

Abstract

Poly(3-hydroxybutyrate-c-3-hydroxyvalerate)(P3HBV)-b-poly(3-hydroxybutyrate) (P3HB)-b-P3HBV triblock copolymer biosynthesized from Ralstonia eutropha was investigated with respect to crystallization and compared with P3HBV-b-P3HB diblock copolymer. First, P3HBV and second, P3HB blocks lengths were fixed, and the third P3HBV block length was changed by regulating the cultivation time. The fraction of P3HB sandwiched by P3HBV blocks was between 50.0 and 86.4%. For the triblock copolymer with a longer third P3HBV block, there was no exothermic crystallization peak cooling from the melt at 200 °C. However, the diblock copolymer provided P3HB crystallization. Therefore, the P3HB block sandwiched by P3HBV with a sufficiently long cultivation time prevented crystallization during the melt-cooling process. Although the crystallization was prevented just after cooling from the melt for the triblock copolymer, it was gradually promoted within a few hours. This shows that the crystallization rate of P3HB is very low because of P3HV blocks.

Keywords

Poly(3-hydroxybutyrate) Poly(3-hydroxyvalerate) Biosynthesis Triblock copolymer Crystallization 

Notes

Acknowledgement

This work was carried out by the financial support from Ryukoku University.

References

  1. 1.
    Kamiya N, Yamamoto Y, Inoue Y, Chujo R, Doi Y (1989) Macromolecules 22:1676CrossRefGoogle Scholar
  2. 2.
    Holmes PA (1985) Phys Technol 16:32CrossRefGoogle Scholar
  3. 3.
    Doi Y, Tamaki A, Kunioka M, Soga K (1987) J Chem Soc Chem Commun 21:1635CrossRefGoogle Scholar
  4. 4.
    Doi Y, Tamaki A, Kunioka M, Soga K (1998) Appl Microbiol Biotechnol 28:330CrossRefGoogle Scholar
  5. 5.
    Mitomo H, Morishiota N, Doi Y (1993) Macromolecules 26:5809CrossRefGoogle Scholar
  6. 6.
    Yoshie N, Menju H, Sato H, Inoue Y (1995) Macromolecules 28:6516CrossRefGoogle Scholar
  7. 7.
    Yoshie N, Fujiwara M, Kasuya K, Abe H, Doi Y, Inoue Y (1999) Macromol Chem Phys 200:977CrossRefGoogle Scholar
  8. 8.
    Mitomo H, Morishita N, Doi Y (1995) Polymer 36:2573CrossRefGoogle Scholar
  9. 9.
    Scandola M, Ceccorulli G, Pizzoli M, Gazzano M (1992) Macromolecules 25:1405CrossRefGoogle Scholar
  10. 10.
    Abe H, Doi Y, Kumagai Y (1994) Macromolecules 27:6012CrossRefGoogle Scholar
  11. 11.
    Andrade AP, Neuenschwander P, Hany R, Egli T, Witholt B (2002) Z, Li. Macromolecules 35:4946CrossRefGoogle Scholar
  12. 12.
    Ravenelle F, Marchessault RH (2002) Biomacromol 3:1057CrossRefGoogle Scholar
  13. 13.
    Shuai X, Jedlinski Z, Luo Q, Farhod N (2000) Chin J Polym Sci 18:19Google Scholar
  14. 14.
    Kumagai Y, Doi Y (1993) J Environ Polym Degrad 1:81CrossRefGoogle Scholar
  15. 15.
    Pederson EN, McChalicher CWJ, Srienc F (2006) Biomacromol 7:1904CrossRefGoogle Scholar
  16. 16.
    Hu D, Chung AL, Wu LP, Zhang X, Wu Q, Chen JC, Chen GQ (2011) Biomacromol 12:3166CrossRefGoogle Scholar
  17. 17.
    Tappel RC, Kucharski JM, Mastroianni JM, Stipanovic AJ, Nomur CT (2012) Biomacromol 12:2964CrossRefGoogle Scholar
  18. 18.
    Tripathi L, Wu L-P, Meng D, Chen J, Chen G-Q (2013) Biomacromol 14:862CrossRefGoogle Scholar
  19. 19.
    Wang Q, Yang P, Liu C, Xue Y, Xian M, Zhao G (2013) Bioresour Technol 131:548CrossRefGoogle Scholar
  20. 20.
    Li S, Cai L, Wu L, Zeng G, Chen J, Wu Q, Chen G-Q (2014) Biomacromol 15:2310CrossRefGoogle Scholar
  21. 21.
    Nakaoki T, Yamagishi R, Ishii D (2015) J Polym Environ 23:487CrossRefGoogle Scholar
  22. 22.
    Kobayashi M, Akita K, Tadokoro H (1968) Makromol Chem 118:324CrossRefGoogle Scholar
  23. 23.
    Kissin YV, Tsvetkova VI, Chirkov NM (1972) Eur Polym J 8:529CrossRefGoogle Scholar
  24. 24.
    Kissin YV (1975) Adv Polym Sci 15:92–155Google Scholar
  25. 25.
    Kissin YV, Rishina LA (1976) Eur Polym J 12:757CrossRefGoogle Scholar
  26. 26.
    Reddy KR, Tashiro K, Sakurai T, Yamaguchi N, Sasaki S, Masunaga H, Takata M (2009) Macromolecules 42:4191CrossRefGoogle Scholar
  27. 27.
    Nakaoki T, Fukui D (2013) J Mol Struct 1051:271CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Materials ChemistryRyukoku University, SetaOtsuJapan

Personalised recommendations