Journal of Polymers and the Environment

, Volume 27, Issue 9, pp 2098–2104 | Cite as

Preparation of Chitooligosaccharide by Hydrogen Peroxide Degradation of Chitosan and Its Effect on Soybean Seed Germination

  • Nguyen Thi Thanh Hai
  • Le Hoai Thu
  • Nguyen Thi Thanh Nga
  • Tran Thai HoaEmail author
  • Le Nghiem Anh Tuan
  • Dang Van Phu
  • Nguyen Quoc HienEmail author
Brief Communication


Chitooligosaccharides (COS) were prepared by hydrogen peroxide (1%) degradation of chitosan solution (3%) in dilute lactic acid at ambient temperature. The molecular weights of degraded chitosan were measured by gel permeation chromatography, and the chemical structure of resultant COS were characterized by Fourier transform infrared spectra. The decrease of chitosan Mw followed the first-order kinetics with the rate constant of 5.71 × 10−7 mol/g/h. As a result, the COS with Mw of 4500 g/mol was obtained at 360 h of the reaction time and its chemical structure almost remained the same as initial chitosan. Germination ability of soybean seeds pre-soaking in COS (4500 g/mol) solution with different concentrations, particularly 0 (control), 50, 100 and 200 mg/L was investigated. Results indicated that germination rate increased significantly for all soybean seeds treated with COS compared to the control. Thus, COS prepared by this method can be potentially applied to large-scale production with reasonably low cost and used as an effective biostimulant for sustainable agriculture.


Chitooligosaccharide Chitosan Hydrogen peroxide Degradation Soybean Germination 



This research is funded by Vietnam National Foundation for Science and Technology Development (NAFOSTED) under Grant No. “104.06-2017.311”.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Nguyen Thi Thanh Hai
    • 1
  • Le Hoai Thu
    • 1
  • Nguyen Thi Thanh Nga
    • 1
  • Tran Thai Hoa
    • 1
    Email author
  • Le Nghiem Anh Tuan
    • 2
  • Dang Van Phu
    • 2
    • 3
  • Nguyen Quoc Hien
    • 3
    Email author
  1. 1.College of SciencesHue UniversityHue CityVietnam
  2. 2.Vietnam Academy of Science and TechnologyGraduate University of Science and TechnologyHanoi CityVietnam
  3. 3.Research and Development Center for Radiation TechnologyVietnam Atomic Energy InstituteHo Chi Minh CityVietnam

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