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Journal of Polymers and the Environment

, Volume 27, Issue 8, pp 1629–1641 | Cite as

A Novel Bacterial Polymeric Silk-Like Protein from a Petroleum Origin Bacillus sp. strain NE: Isolation and Characterization

  • Elbadawy A. KamounEmail author
  • Gadallah M. Abu-Elreesh
  • Esmail M. El-Fakharany
  • Desouky Abd-El-HaleemEmail author
Original paper
  • 127 Downloads

Abstract

This paper explores a novel silk-like protein (named Bacillus NE silk (BNES)) isolated from a petroleum origin bacterial strain belongs to the genus Bacillus. The successful characterizations for its chemical structure determination and protein composition have been performed. The results demonstrate that BNES protein is a new type of silk-like protein and has a prospective for applying as a new bacterial silk-like biomaterial. The amino acids constituents of silk-like protein showed that (Ala-Pro-Gly) rich-block protein, are the main major amino acids, while the protein Mwt., was recorded around > ~ 400 kDa, which excelled those similar of natural silk. The degummed or purified silk-like protein was successfully electro-spinned for wet-electrospun silk-like nanofibers using electrospinning technique. Silk-like nanofibers gave an adequate and uniform ribbon-shape structure with average diameter of nanofiber around 110 nm. Meanwhile, the block silk-like protein exhibited high thermal degradation monitored at 140–373 °C, which close those of natural silkworm silk. The purified silk-like protein showed no toxic effect against Vero kidney normal cell-line even after 48 h of incubation, using MTT-assay test. The obtained results demonstrate that, silk-like protein from wild type bacterial origin has potential for use as novel fibrous biomaterials for biomedical and environmental applications.

Keywords

Silk-like protein Petroleum origin Bacillus sp. Isolation Characterization 

Notes

Compliance with ethical standards

Competing interests

The authors declare no competing or financial interests.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Elbadawy A. Kamoun
    • 1
    • 2
    Email author
  • Gadallah M. Abu-Elreesh
    • 3
  • Esmail M. El-Fakharany
    • 4
  • Desouky Abd-El-Haleem
    • 3
    Email author
  1. 1.Polymeric Materials Research Dep., Advanced Technology and New Materials Research Institute (ATNMRI)City of Scientific Research and Technological Applications (SRTA-City)New Borg Al-Arab City, AlexandriaEgypt
  2. 2.Nanotechnology Research Center (NTRC)The British University in Egypt (BUE)El-Sherouk CityEgypt
  3. 3.Environmental Biotechnology Research Dep., Genetic Engineering and Biotechnology Research Institute (GEBRI)City of Scientific Research and Technological Applications (SRTA-City)New Borg Al-Arab City, AlexandriaEgypt
  4. 4.Therapeutic and Protective Proteins Laboratory, Proteins Research Dep., Genetic Engineering and Biotechnology Research Institute (GEBRI)City of Scientific Research and Technological Applications (SRTA-City)New Borg Al-Arab City, AlexandriaEgypt

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