Physico-chemical Characterization of Poly(3-Hydroxybutyrate) Produced by Halomonas salina, Isolated from a Hypersaline Microbial Mat
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In this work, the characterization of poly(3-hydroxybutyrate) PHB produced by Halomonas salina isolated from a hypersaline microbial mat from Guerrero Negro, Baja California Sur, Mexico, is reported. The bacterial strain was able to produce isotactic PHB biopolymer with glucose (1%) as a single carbon source. The chemical structure of the polymer obtained was confirmed by Fourier-transform infrared (FTIR) and Nuclear magnetic resonance (NMR) spectroscopy. The polymer was thermally stable up to 225 °C and Differential scanning calorimetry (DSC) analysis showed a melting temperature (Tm) of 173.6 °C. The obtained polymer presented a lower degree of crystallinity (39.3%) in comparison with PHB produced by other bacteria and polyhydroxyalkanoate co-polymers. Thus, the PHB biopolymer obtained in this study, could be recognized as more suitable for practical use, contributing to the repertoire of available bioplastics for further potential biotechnological applications, in which elastic polymers are needed.
KeywordsPoly(3-hydroxybutyrate) Halomonas salina Biopolymer Hypersaline microbial mats
We thank to LANNBIO CINVESTAV-Merida (projects FOMIX-Yucatan 2008-108160, CONACYT LAB-2009-01 No. 123913 and CB20121 178947) for the TGA, XRD, NMR and SEM-EDX measurements. The authors acknowledge the Cátedras CONACYT projects 3139 and 1568. We highly appreciate the technical support of Hever Latisnere-Barragán in the laboratory (CIBNOR) and Dr Wilberth Herrera Kao for assistance with GPC measurements.
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