Improved Chemical Reactivity of Lignocellulose from High Solids Content Micro-fibrillation by Twin-screw Extrusion

  • Jinlei Li
  • Michael ThompsonEmail author
  • David J. W. Lawton
Original Paper


The low reactivity of lignocellulose limits the effective chemical conversion of lignocellulose biomass into functional bioproducts. Mechanical micro-fibrillation treatment can improve the chemical accessibility of lignocellulose but usually has limited productivity by the low processing solids content. The presented work demonstrates effective micro-fibrillation of lignocellulose at high solids content up to 60 wt% can be achieved by twin-screw extrusion. Morphological characterizations of the extruded wood pulp lignocellulose show the degree of micro-fibrillation is enhanced by operating at higher solids content. The lignocellulose treated at 60 wt% solids content presents 2.1 and 4.8 times higher water retention capacity and specific surface area, respectively, than the original material. Acetylation results show the twin-screw extrusion pre-treatment can significantly accelerate the chemical modification of lignocellulose by 50%. This high productivity method for micro-fibrillating lignocellulose should be of great interest to the bioplastics industry.


Lignocellulose Twin-screw extrusion Micro-fibrillation Chemical reactivity 



This study was supported by Clean Manufacturing and Nano-engineering of Sustainable Materials, Ontario Research Fund (ORF) led by Dr. Sain at the University of Toronto.


  1. 1.
    Isikgor FH, Becer CR (2015) Lignocellulosic biomass: a sustainable platform for the production of bio-based chemicals and polymers. Polym Chem 6:4497–4559CrossRefGoogle Scholar
  2. 2.
    Brodin M, Vallejos M, Opedal MT et al (2017) Lignocellulosics as sustainable resources for production of bioplastics—a review. J Clean Prod 162:646–664CrossRefGoogle Scholar
  3. 3.
    Ten E, Vermerris W (2013) Functionalized polymers from lignocellulosic biomass: state of the art. Polymers (Basel) 5:600–642CrossRefGoogle Scholar
  4. 4.
    Chen J, Tang C, Yue Y et al (2017) Highly translucent all wood plastics via heterogeneous esterification in ionic liquid/dimethyl sulfoxide. Ind Crops Prod 108:286–294CrossRefGoogle Scholar
  5. 5.
    Chen MJ, Li RM, Zhang XQ et al (2017) Homogeneous transesterification of sugar cane bagasse toward sustainable plastics. ACS Sustain Chem Eng 5:360–366CrossRefGoogle Scholar
  6. 6.
    Zhen L, Zhang G, Huang K et al (2016) Modification of rice straw for good thermoplasticity via graft copolymerization of ε—caprolactone onto acetylated rice straw using ultrasonic-microwave coassisted technology. ACS Sustain Chem Eng 4:957–964CrossRefGoogle Scholar
  7. 7.
    Bao L, Zou X, Chi S et al (2018) Advanced sustainable thermoplastics based on wood residue using interface nanomodification technique. Adv Sustain Syst 1800050:1–12Google Scholar
  8. 8.
    Tian SQ, Zhao RY, Chen ZC (2018) Review of the pretreatment and bioconversion of lignocellulosic biomass from wheat straw materials. Renew Sustain Energy Rev 91:483–489CrossRefGoogle Scholar
  9. 9.
    Lee SH, Teramoto Y, Endo T (2010) Enhancement of enzymatic accessibility by fibrillation of woody biomass using batch-type kneader with twin-screw elements. Bioresour Technol 101:769–774CrossRefGoogle Scholar
  10. 10.
    Hoeger IC, Nair SS, Ragauskas AJ et al (2013) Mechanical deconstruction of lignocellulose cell walls and their enzymatic saccharification. Cellulose 20:807–818CrossRefGoogle Scholar
  11. 11.
    Duque A, Manzanares P, Ballesteros M (2017) Extrusion as a pretreatment for lignocellulosic biomass: Fundamentals and applications. Renew Energy 114:1427–1441CrossRefGoogle Scholar
  12. 12.
    Lee SH, Teramoto Y, Endo T (2009) Enzymatic saccharification of woody biomass micro/nanofibrillated by continuous extrusion process I—effect of additives with cellulose affinity. Bioresour Technol 100:275–279CrossRefGoogle Scholar
  13. 13.
    Leu S-Y, Zhu JY (2013) Substrate-related factors affecting enzymatic saccharification of lignocelluloses: our recent understanding. Bioenergy Res 6:405–415CrossRefGoogle Scholar
  14. 14.
    Kumar AK, Sharma S (2017) Recent updates on different methods of pretreatment of lignocellulosic feedstocks: a review. Bioresour Bioprocess 4:7CrossRefGoogle Scholar
  15. 15.
    Zheng J, Rehmann L (2014) Extrusion pretreatment of lignocellulosic biomass: a review. Int J Mol Sci 15:18967–18984CrossRefGoogle Scholar
  16. 16.
    Kratky L, Jirout T (2011) Biomass size reduction machines for enhancing biogas production. Chem Eng Technol 34:391–399CrossRefGoogle Scholar
  17. 17.
    Chen X, Kuhn E, Wang W et al (2013) Comparison of different mechanical refining technologies on the enzymatic digestibility of low severity acid pretreated corn stover. Bioresour Technol 147:401–408CrossRefGoogle Scholar
  18. 18.
    Baati R, Magnin A, Boufi S (2017) High solid content production of nanofibrillar cellulose via continuous extrusion. ACS Sustain Chem Eng 5:2350–2359CrossRefGoogle Scholar
  19. 19.
    Rol F, Karakashov B, Nechyporchuk O et al (2017) Pilot-scale twin screw extrusion and chemical pretreatment as an energy-efficient method for the production of nanofibrillated cellulose at high solid content. ACS Sustain Chem Eng 5:6524–6531CrossRefGoogle Scholar
  20. 20.
    Ho TTT, Abe K, Zimmermann T, Yano H (2015) Nanofibrillation of pulp fibers by twin-screw extrusion. Cellulose 22:421–433CrossRefGoogle Scholar
  21. 21.
    Choi CH, Kim JS, Oh KK (2013) Evaluation the efficacy of extrusion pretreatment via enzymatic digestibility and simultaneous saccharification &fermentation with rapeseed straw. Biomass Bioenerg 54:211–218CrossRefGoogle Scholar
  22. 22.
    Choi CH, Oh KK (2012) Application of a continuous twin screw-driven process for dilute acid pretreatment of rape straw. Bioresour Technol 110:349–354CrossRefGoogle Scholar
  23. 23.
    Um BH, Choi CH, Oh KK (2013) Chemicals effect on the enzymatic digestibility of rape straw over the thermo-mechanical pretreatment using a continuous twin screw-driven reactor (CTSR). Bioresour Technol 130:38–44CrossRefGoogle Scholar
  24. 24.
    da Silva AS, Teixeira RSS, Endo T et al (2013) Continuous pretreatment of sugarcane bagasse at high loading in an ionic liquid using a twin-screw extruder. Green Chem 15:1991–2001CrossRefGoogle Scholar
  25. 25.
    Vandenbossche V, Brault J, Hernandez-Melendez O et al (2016) Suitability assessment of a continuous process combining thermo-mechano-chemical and bio-catalytic action in a single pilot-scale twin-screw extruder for six different biomass sources. Bioresour Technol 211:146–153CrossRefGoogle Scholar
  26. 26.
    Liu W, Wang B, Hou Q et al (2016) Effects of fibrillation on the wood fibers’ enzymatic hydrolysis enhanced by mechanical refining. Bioresour Technol 206:99–103CrossRefGoogle Scholar
  27. 27.
    Zhang L, Lu H, Yu J et al (2017) Dissolution of lignocelluloses with high lignin content in a NMMO/H2O solvent system via a simple glycerol swelling and mechanical pretreatment. J Agric Food Chem 65:9587–9594CrossRefGoogle Scholar
  28. 28.
    Cha YL, Yang J, Seo S, Il et al (2016) Alkaline twin-screw extrusion pretreatment of Miscanthus with recycled black liquor at the pilot scale. Fuel 164:322–328CrossRefGoogle Scholar
  29. 29.
    Kim TH, Choi CH, Oh KK (2013) Bioconversion of sawdust into ethanol using dilute sulfuric acid-assisted continuous twin screw-driven reactor pretreatment and fed-batch simultaneous saccharification and fermentation. Bioresour Technol 130:306–313CrossRefGoogle Scholar
  30. 30.
    Liu C, Van Der Heide E, Wang H et al (2013) Alkaline twin-screw extrusion pretreatment for fermentable sugar production. Biotechnol Biofuels 6:1–11CrossRefGoogle Scholar
  31. 31.
    Senturk-Ozer S, Gevgilili H, Kalyon DM (2011) Biomass pretreatment strategies via control of rheological behavior of biomass suspensions and reactive twin screw extrusion processing. Bioresour Technol 102:9068–9075CrossRefGoogle Scholar
  32. 32.
    Velásquez-Cock J, Gañán P, Gómez HC et al (2018) Improved redispersibility of cellulose nanofibrils in water using maltodextrin as a green, easily removable and non-toxic additive. Food Hydrocoll 79:30–39CrossRefGoogle Scholar
  33. 33.
    Spence KL, Venditti RA, Rojas OJ et al (2010) The effect of chemical composition on microfibrillar cellulose films from wood pulps: water interactions and physical properties for packaging applications. Cellulose 17:835–848CrossRefGoogle Scholar
  34. 34.
    Samiey B, Dargahi MR (2010) Kinetics and thermodynamics of adsorption of congo red on cellulose. Cent Eur J Chem 8:906–912Google Scholar
  35. 35.
    Qin Y, Qiu X, Zhu JY (2016) Understanding longitudinal wood fiber ultra-structure for producing cellulose nanofibrils using disk milling with diluted acid prehydrolysis. Sci Rep 6:35602CrossRefGoogle Scholar
  36. 36.
    Gu F, Wang W, Cai Z et al (2018) Water retention value for characterizing fibrillation degree of cellulosic fibers at micro and nanometer scales. Cellulose 25:2861–2871CrossRefGoogle Scholar
  37. 37.
    Kekäläinen K, Liimatainen H, Illikainen M et al (2014) The role of hornification in the disintegration behaviour of TEMPO-oxidized bleached hardwood fibres in a high-shear homogenizer. Cellulose 21:1163–1174CrossRefGoogle Scholar
  38. 38.
    Yano H, Nakahara S (2004) Bio-composites produced from plant microfiber bundles with a nanometer unit web-like network. J Mater Sci 39:1635–1638CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Jinlei Li
    • 1
  • Michael Thompson
    • 1
    Email author
  • David J. W. Lawton
    • 2
  1. 1.Department of Chemical EngineeringMcMaster UniversityHamiltonCanada
  2. 2.Xerox Research Centre of CanadaMississaugaCanada

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