Biosynthesis of Poly(3-hydroxybutyrate) from Cheese Whey by Bacillus megaterium NCIM 5472
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Microbial polyhydroxyalkonate such as homopolyester of poly(3-hydroxybutyrate) (PHB) was produced from cheese whey by Bacillus megaterium NCIM 5472. Due to their numerous potential industrial applications, the focus was given to competently enhance the amount of PHB produced. The amount of PHB produced from whole cheese whey, and ultrafiltered cheese whey was first compared, and after observing a rise in PHB production by using ultrafiltered cheese whey, cheese whey permeate was chosen for further analysis. The presence of PHB was then confirmed by GCMS. Since the main aim of the study was to increase the amount of PHB produced through batch fermentation, various process parameters like time, pH, C/N ratio, etc. were optimized. After optimization, it was found that B. megaterium NCIM 5472 was capable of accumulating 75.5% of PHB of its dry weight and a PHB yield of 8.29 g/L. The chemical structure of the polymer was further analyzed by using FTIR and NMR spectroscopy methods. Also, the physical and thermal properties were studied by using Differential scanning calorimetry and Thermogravimetric analysis. It was found that the polymer produced had excellent thermal stability, thus allowing the possibility to exploit its properties for industrial purposes such as adhesives, packaging materials, etc.
KeywordsBiopolymer Poly(3-hydroxybutyrate) Bacillus megaterium Cheese whey permeate
The authors wish to express their sincere thanks to the management of SRM Institute of Science and Technology and Department of Biotechnology for the research facilities and their constant support throughout this research study. We would also like to thank Dr. M. Venkatesh Prabhu, Assistant Professor (S.G) for his technical help during this research.
- 5.Chanprateep S, Katakura Y, Visetkoop S, Shimizu H, Kulpreecha S, Shioya S (2008) Characterization of new isolated Ralstonia eutropha strain A-04 and kinetic study of biodegradable copolyester poly(3-hydroxybutyrate-co-4-hydroxybutyrate) production. J Ind Microbiol Biotechnol 35(11):1205–1215CrossRefGoogle Scholar
- 22.Alkotaini B, Sathiyamoorthi E, Kim BS (2015) Potential of Bacillus megaterium for production of polyhydroxyalkanoates using the red algae Gelidium amansii. Int J Biol Macromol 20:856–860Google Scholar
- 23.Irsath H, Santhosh S, Hemalatha V, Vikramathithan M, Dhanasekar R, Dhandapani R (2015) Production and optimization of polyhydroxybutyrate using Bacillus subtilis BP1 isolated from sewage sample. Int J Pure Appl Biosci 3(1):158–166Google Scholar
- 30.Greenberg AE, Clesceri LS, Eaton AD (1995) Standard methods for the examination of water and wastewater, 19th ed. American Public Health Association, Washington, DCGoogle Scholar
- 37.Mohapatra S, Samantaray DP, Samantaray SM, Mishra BB, Das S, Majumdar S, Pradhan SK, Rath SN, Rath CC, Akthar J, Achary KG (2013) Structural and thermal characterization of PHAs produced by Lysinibacillus sp. through submerged fermentation process. Int J Biol Macromol 93(Pt A):1161–1167Google Scholar
- 40.Shah KR (2012) FTIR analysis of polyhydroxyalkanoates by a locally isolated novel Bacillus sp. AS 3-2 from soil of Kadi region, North Gujarat, India. J Biochem Technol 3(4):380–383Google Scholar
- 43.Babruwad PR, Prabhu SU, Upadhyaya KP, Hungund BS (2015) Production and characterization of thermostable polyhydroxybutyrate from Bacillus cereus PW3A. J Biochem Technol 6(3):990–995Google Scholar