Optimization and Characterisation of Thermo Stable Exopolysaccharide Produced from Bacillus licheniformis WSF-1 Strain
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Bacillus licheniformis WSF-1 strain isolated from sugar distillery plant was capable of producing a maximum amount of EPS (1.24 g/mL) and dry biomass (3.25 g/mL) respectively. Certain factors like inoculum age and carbon source was standardised in view of obtaining high EPS yield. In this study, 48-h-old culture was identified as the optimum inoculum age for EPS production. In fermentation, as carbon source plays a major contributing factor that influences the EPS production, optimum carbon source was determined by studying the effect of different carbohydrates such as 12% of glucose, sucrose, lactose, maltose, xylose and fructose on EPS production. The best carbon source was identified as sucrose at a concentration of 25% producing 2.9 g/mL of exopolysaccharide. The polysaccharides were hydrolysed and the monomeric components were determined as fructose and glucose by HPLC, followed by FT-IR analysis. The thermal study revealed that the EPS can withstand high temperature of 219.4 °C and had high glass transition temperature of 150.6 °C. These features indicate the thermo stable nature of the exopolysaccharide which can be used for various industrial applications.
KeywordsExopolysaccharide Bacillus Thermo stable Polymer Carbon source FTIR SEM
This work was supported by the University Grants Commission-Maulana Azad National Fellowship for minority students with Reference Number F1-17.1/2011/MANF-CHR-TAM-562/(SA-III/Wesite) dated 2nd Jan 2012. The authors would like to thank Periyar University for providing sophisticated laboratory facilities to carry out this research work. The authors also sincerely show gratitude to DST-FIST, New Delhi, India for granting sophisticated instrumentation with Reference No. SR/FST/LSI – 640/2015 (C) dated 30/5/2016.
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Conflict of interest
All authors declare that they have no conflict of interest.
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