Emulsifying and Cell Proliferative Abilities of the Exopolysaccharide Produced by Leguminous Plant Nodule Associated Bacterium Cronobacter sp.
- 23 Downloads
Exopolysaccharides (EPS) produced by bacteria are high molecular weight secondary metabolites having wide applications in food and pharmaceutical industry. In the present study, an exopolysaccharide producing bacterium was isolated from the root nodules of a leguminous plant and was studied for emulsification activity and biocompatibility using human dermal fibroblasts. Under optimum culture conditions, the purified EPS yield in modified YEM media was 2.8 g L−1 and was identified as β-glucan with the molecular weight 15.57 × 106 Da. The EPS showed high emulsifying activity against hydrocarbons such as petrol, kerosene, xylene, palm oil, coconut oil, and olive oil with emulsification index > 60% at 0.25 and 0.5% concentrations at 25 °C. The EPS was sensitive to high temperature, but the emulsions were stable at pH range 2–9 and in the salinity range of 1–20%. In vitro cell viability studies indicated that EPS was not cytotoxic at tested concentrations on human dermal fibroblasts moreover, it had proliferative activities. Hence, the EPS from Cronobacter sp. can be a good candidate for biotechnological application as a source of β-glucan with emulsifying and tissue regenerative properties.
KeywordsRoot nodule Hydrocarbons MTT assay Gel permeation chromatography
The authors acknowledge Prof CC Young, Chung-Hsing University, Taiwan for 16S rRNA gene sequencing. Fathimath Sadiya MK acknowledges Yenepoya University for the research fellowship and Sahana TG acknowledges DST INSPIRE fellowship.
Compliance with Ethical Standards
Conflict of interest
All authors have declared that they have no conflict of interest.
- 1.Poli A, Di Donato P, Abbamondi GR, Nicolaus B (2011) Synthesis, production, and biotechnological applications of exopolysaccharides and polyhydroxyalkanoates by archaea. Archaea 10:e693253Google Scholar
- 4.De Almeida DG, Soares Da Silva RD, Luna JM, Rufino RD, Santos VA, Banat IM, Sarubbo LA (2016) Biosurfactants: promising molecules for petroleum biotechnology advances. Front Microbiol 7:e1718Google Scholar
- 5.Lopes EM, Castellane TC, Moretto C, Lemos EG, Souza JA (2014) Emulsification properties of bioemulsifiers produced by wild-type and mutant Bradyrhizobium elkanii strains. J Bioremediat Biodegrad 20(5):1–6Google Scholar
- 7.Kaur V, Bera MB, Panesar BS, Chopra HK (2013) Production and characterization of exopolysaccharide produced by Alcaligenes faecalis B14 isolated from indigenous soil. Int J Biotechnol Bioeng Res 4:365–374Google Scholar
- 14.Senni K, Gueniche F, Yousfi M, Fioretti F, Godeau GJ, Colliec-Jouault S et al (2013) Sulfated depolymerized derivatives of expolysaccharides (EPS) from mesophilic marine bacteria, method for preparing the same, and uses thereof in tissue regeneration. United States patent US 8,598,142Google Scholar
- 19.Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193(1):265–275Google Scholar
- 20.Lee JS, Lee SU, Che CY, Lee JE (2015) Comparison of cytotoxicity and wound healing effect of carboxymethylcellulose and hyaluronic acid on human corneal epithelial cells. Int J Ophthalmol 8(2):215–221Google Scholar
- 23.Joseph S, Cetinkaya E, Drahovska H, Levican A, Figueras MJ, Forsythe SJ (2012) Cronobacter condimenti sp. nov., isolated from spiced meat, and Cronobacter universalis sp. nov., a species designation for Cronobacter sp. genomospecies, recovered from a leg infection, water and food ingredients. Int J Syst Evol Microbiol 62(6):1277–1283CrossRefGoogle Scholar
- 27.Nishinari K, Zhang H, Ikeda S (2001) Hydrocolloid gels of polysaccharides and proteins. Curr Opin Colloid Interface Sci 3:195–201Google Scholar
- 32.Sun ML, Zhao F, Shi M, Zhang XY, Zhou BC, Zhang YZ et al (2015) Characterization and biotechnological potential analysis of a new exopolysaccharide from the Arctic marine bacterium Polaribacter sp. SM1127. Sci Rep 21(5):e18435Google Scholar
- 33.Alcantara OL, Domenech NA, Astals AS, Montiel AF, Sanz NG (2017) Cosmetic composition containing halomonas ferment extract, and use thereof. United States patent application US 15/521,774Google Scholar