Conferring Antibacterial Properties on Sesbania Gum via Microwave-Assisted Graft Copolymerization of DADMAC
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Microbial contamination, the consequences of both anthropogenic and geogenic activity, includes high health and environmental threats and may adversely affects the ecological niche. The present work is a successful attempt towards the fabrication of anti-bacterial agent by incorporation of cationic monomer, diallyldimethylammonium chloride (DADMAC) on sesbania gum through microwave assisted method. The grafted chains of poly (DADMAC) provide positive charge to sesbania gum which in turn confers antibacterial activity. The synthesized product was characterized through FTIR, SEM, TGA, DSC elemental and viscometric analysis. The inhibitory activity of the synthesized product has been evaluated in vitro against both Gram-positive (Staphylococcus aureus NCIM 2122 and Bacillus anthracis) as well as Gram-negative (Salmonella typhi NCIM 2501 and Escherichia coli NCIM 2832) bacteria through standard agar cup method. Optimum minimum inhibitory concentration for each grade of graft copolymer measured by calculating the zone of inhibition has been found at 750 µg/ml.
KeywordsCationic polymer DADMAC Anti-bacterial activity Minimum inhibitory concentration
Pinki Pal acknowledges the Department of Science and Technology (DST), India, for the research grant (sanction order No. SR/WOS-A/ET-13/2014). The authors also acknowledge the support of CIF-BIT Mesra and CHN lab, Punjab University, Chandigarh. We are also thankful to UGC-CAS, Department of Botany, The University of Burdwan. AB and UH are also thankful for the financial assistance to SRF and JRF accordingly.
- 1.Allan GG, Altman LC, Bensinger RE, Ghose DK, Hirabayashi Y, Neogi AN, Neogi S (1984) Biomedical application of chitin and chitosan, In: Zikakis JP (ed) In: Chitin chitosan and related enzymes. Acedemic Press, New York, pp. 113–119Google Scholar
- 6.Whistler RL (1993) Exudate gums: industrial gums, polysaccharides and their derivatives. Academic Press, San Diego, pp 309–339Google Scholar
- 15.Ceresa RJ (1962) Block & graft copolymer. Butterworths, LondonGoogle Scholar
- 18.Coma V (2008) Current research and developments on chitin and chitosan in biomaterials sciences. Research Signpost, Trivandrum, pp 21–51Google Scholar
- 22.Collins EA, Bares J, Billmeyer FW (1973) Experiments in polymer science. Wiley, New YorkGoogle Scholar
- 24.Miller ML (1996) The structure of polymers. Reinhold Publishing Corporation, New YorkGoogle Scholar
- 25.Razali MAA, Sanusi N, Ismail H, Othman N, Ariffin A (2012) Application of response surface methodology (RSM) for optimization of cassava starch grafted polyDADMAC synthesis for cationic properties. Wiley, New York, pp 1–9Google Scholar