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Circ-TFCP2L1 Promotes the Proliferation and Migration of Triple Negative Breast Cancer through Sponging miR-7 by Inhibiting PAK1

  • Qian Wang
  • Zhouxiao Li
  • Yun Hu
  • Wubin Zheng
  • Weiwei Tang
  • Changyuan Zhai
  • Zhutong Gu
  • Jing TaoEmail author
  • Hanjin WangEmail author
Article
  • 64 Downloads

Abstract

CircRNAs are essential factors that have been verified to regulate various forms of carcinogenesis. However, the role of circRNAs in triple negative breast cancer (TNBC) tumourigenesis is not well clarified. In this study, we explored the circRNA expression profiles and possible modulation mechanism of circRNAs on triple negative breast cancer tumourigenesis. We used three pairs of triple negative breast cancer tissues and adjacent noncancerous tissues to perform a human circRNA microarray for screening of circRNA expression patterns in TNBC. The results showed that circ-TFCP2L1 was significantly up-regulated in TNBC tissues and cells, tending to have a shorter disease-free survival of TNBC patients. In vitro loss-of-function experiments showed that knockdown of circ-TFCP2L1 significantly suppressed the proliferation and migration of TNBC cells. Moreover, the results showed that the proliferation and migration capabilities and PAK1 expression in TNBC cells treated with si-circ-TFCP2L1 + miR-7 mimics were significantly suppressed compared with the normal group. Therefore, circ-TFCP2L1 was identified as a sponge of miR-7 functionally targeting PAK1 and further promoting the proliferation and migration of TNBC cells. Taken together, the results from our study reveal a novel regulatory mechanism and offer novel insight into the role of circ-TFCP2L1 in progression of triple negative breast cancer.

Keywords

circRNAs Microarray Sponge Proliferation Migration 

Notes

Funding Information

This work was supported by the Medical Science and Technology Development Foundation (ZKX18027) for Hanjin Wang and by the Nanjing Municipal Health Planning Commission (YKK17264) for Jing Tao.

Compliance with Ethical Standards

Conflict of Interest

The authors report no conflicts of interest in this work.

Supplementary material

10911_2019_9440_MOESM1_ESM.docx (22 kb)
ESM 1 (DOCX 21 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Qian Wang
    • 1
  • Zhouxiao Li
    • 2
  • Yun Hu
    • 3
  • Wubin Zheng
    • 1
  • Weiwei Tang
    • 1
  • Changyuan Zhai
    • 4
  • Zhutong Gu
    • 5
  • Jing Tao
    • 4
    Email author
  • Hanjin Wang
    • 1
    Email author
  1. 1.Department of General Surgery, Nanjing First HospitalNanjing Medical UniversityNanjingChina
  2. 2.Department of Hand Surgery, Plastic Surgery and Aesthetic SurgeryLudwig-Maximilians UniversityMunichGermany
  3. 3.Department of General SurgeryJiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical UniversityNanjingChina
  4. 4.Department of General Surgery, Nanjing Pukou HospitalNanjing Medical UniversityNanjingChina
  5. 5.Department of General SurgeryNanjing Pukou Hospital, Second Affiliated Hospital of Nanjing Medical UniversityNanjingChina

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