Synthesis, Characterization and Applications of a Novel Platinum-Based Nanoparticles: Catalytic, Antibacterial and Cytotoxic Studies

  • Muhammad Safdar
  • Mehmet Ozaslan
  • Rozhgar A. Khailany
  • Sehrish Latif
  • Yasmeen JunejoEmail author
  • Muhammad Saeed
  • Mustafa S. Al-Attar
  • Belan O. Kanabe


The facile one-pot synthesis method was used to synthesize platinum nanoparticles (Doxy-Pt (0) NPs) by reduction method with doxycycline drug as reducing and capping agent. The combined platinum nanoparticles were carefully chosen for catalytic reduction of paracetamol as well as used against antimicrobial and anticancer actions with the well diffusion process and by measuring the zone of inhibition in mm against Gram-positive (S. aureus and S. pyogenes) and Gram-negative (E. coli and S. typhimurium). The Ultraviolet–Visible-absorption spectroscopy technique was used to endorse accumulation of (Doxy-Pt (0) NPs) using the distinctive Plasmon absorption maxima at 264 nm. Another technique of X-ray powder diffraction (XRD) pattern was used to confirm the crystalline nature of the prepared Pt (0) NPs. Transmission electron microscopy (TEM) was used to study the particle size of the prepared Pt (0) NPs with a size range of 10–20 nm. The catalytic study showed that the reduction is ~ 99.9% of paracetamol that was consummate in a small period of reaction time (60 s) by competent doxycycline based platinum nanoparticles. The value of K is calculated (rate constant) value for paracetamol catalytic reduction was achieved 7 × 10−2 by drawing InC versus time (s) and the results showed that the reaction is of the first command kinetics. Furthermore, the reduction in breast cancer cell viability and proliferation via PtNPs was found higher as compare to the normal breast cells (CRL-4010). Therefore, the newly prepared Doxy-Pt (0) NPs had a marvelous catalytic, antibacterial and anticancerous action as a catalyst and drug material. The recent discoveries are similarly extendable for the preservation of the aquatic situation in contradiction of the pollution produced by paracetamol, microbial and cancerous actions via a superficial, highly inexpensive, quick and effectual technique.

Graphic Abstract


Platinum nanoparticles Synthesis Characterization Degradation of paracetamol Antimicrobial activity Cytotoxicity 



Doxycycline derived platinum nanoparticles


Ultraviolet–Visible spectroscopy


X-ray diffraction


Transmission electron microscopy

S. aureus

Staphylococcus aureus

S. pyogens

Streptococcus pyogenes

E. coli

Escherichia coli

S. typhimurium

Salmonella typhimurium


Nonsteroidal anti-inflammatory drugs


Colony-forming unit



Dr. Yasmeen Junejo would like to thank HEC-Pakistan for providing Assistant Professor (IPFP) position and Dr. Ghulam Shabir, Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan for giving us bacterial strains and the necessary facilities.

Author Contributions

YJ, and MS designed and performed the experiments. SL and RAK performed the measurements. YJ and MS analyzed the measurement data. MS finalized the manuscript. All authors read and approved the final manuscript.


The study was supported by departmental research grant.

Compliance with Ethical Standards

Conflict of interests

The authors declare that they have no competing interests.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Muhammad Safdar
    • 1
    • 2
  • Mehmet Ozaslan
    • 2
  • Rozhgar A. Khailany
    • 3
    • 4
  • Sehrish Latif
    • 5
  • Yasmeen Junejo
    • 6
    Email author
  • Muhammad Saeed
    • 7
  • Mustafa S. Al-Attar
    • 8
  • Belan O. Kanabe
    • 2
  1. 1.Department of Breeding and Genetics, Faculty of Animal Production & TechnologyCholistan University of Veterinary & Animal SciencesBahawalpurPakistan
  2. 2.Department of Biology, Division of Molecular Biology and GeneticsGaziantep UniversityGaziantepTurkey
  3. 3.Department of Biology, College of ScienceSalahaddin University-ErbilErbilIraq
  4. 4.Department of Biology, Faculty of EducationTishk International UniversityErbilIraq
  5. 5.Department of BiotechnologyVirtual University of PakistanLahorePakistan
  6. 6.Department of Physiology and Biochemistry, Faculty of Bio-SciencesCholistan University of Veterinary & Animal SciencesBahawalpurPakistan
  7. 7.Department of Poultry Science, Faculty of Animal Production & TechnologyCholistan University of Veterinary & Animal SciencesBahawalpurPakistan
  8. 8.Department of Environmental Science, College of ScienceSalahaddin University-ErbilErbilIraq

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