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Organic–Inorganic Hybrid Nanocomposites: A Novel Way to Immobilize l-Glutamate Oxidase with Manganese Phosphate

  • Yuchi Lu
  • Yan Chen
  • Qingling Wang
  • Xinrui Hao
  • Pengfu LiuEmail author
  • Xiaohe ChuEmail author
Article
  • 28 Downloads

Abstract

\(\alpha\)-Ketoglutaric acid is a versatile chemical. Traditional methods for producing \(\alpha\)-ketoglutaric acid rely on certain some enzymes, but these methods have disadvantages related to difficult separation and high costs. Therefore, it is necessary to make improvements based on the original route. The novel immobilized enzyme, nanocomposite is a relatively superior method. Manganese-l-glutamate oxidase hybrid nanocomposites (Mn-LGOX) were obtained, characterized and optimized in terms of their structural and enzymatic properties. Field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) assays were used to discover and confirm that l-glutamate oxidase (LGOX) was successfully assembled with manganese phosphate. In addition, the nanocomposites exhibited improved resistance to temperature and pH and increased recyclability compared to the earlier free enzyme catalyst method. After recycling six times, the activity of the nanocomposites was maintained over 30% more compared to the original more. In short, the hybrid nanocomposites ideally improve the structural character, the resistance to pH and temperature in the reaction and the storage stability. These data emphasize that Mn-LGOX nanocomposites show great potential for industrial bioconversion

Keywords

l-Glutamate oxidase Hybrid nanocomposites Manganese phosphate Immobilized enzyme HPLC 

Notes

Acknowledgements

This work was supported by Laboratory of Steroid Hormone Development, Institute of Biopharmaceuticals, Green Pharmaceutical Collaborative Innovation Center of Yangtze River Delta, Zhejiang Univ. of Technol. The strain was kindly provided by Zhejiang Lvchuang Biotech Co., Ltd.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Green Pharmaceutical Collaborative Innovation Center of Yangtze River DeltaZhejiang University of TechnologyHangzhouPeople’s Republic of China

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