Synthesis of Tobramycin Stabilized Silver Nanoparticles and Its Catalytic and Antibacterial Activity Against Pathogenic Bacteria

  • Yasmeen JunejoEmail author
  • Muhammad Safdar
  • M. Asad Akhtar
  • Muthupandian Saravanan
  • Haseeb Anwar
  • Muhammad Babar
  • Rabia Bibi
  • M. Tariq Pervez
  • Tanveer Hussain
  • Masroor E. Babar


In the present study, tobramycin stabilized silver nanoparticles (Tob-AgNPs) were effectively synthesized in an aqueous medium by well-organized one-pot technique and successfully employed for the ultra-fast catalytic reduction of 4-nitrophenol (4-NP) and 4-nitroaniline (4-NA). Moreover, the perfection was attained in percent reduction of toxic 4-NP, 4-NA and their mixtures by changing and adjusting reaction period, quantity and concentration of catalyst. Interestingly, this research has shown that comprehensive ~ 99.9% reduction of nitroaromatic was achieved in short reaction time (60 s) by proficient Tob-AgNPs. Remarkably, the calculated rate constant (K) value for 4-NP, 4-NA and their mixtures catalytic reduction was achieved in 7 × 10−2 S−1, 10 × 10−2 S−1 and 8 × 10−2 S−1, respectively by plotting lnC versus time (s). Subsequently, the antibacterial efficacy of Tob-AgNPs was assessed alongside for the selected gram-positive and Gram-negative bacterial pathogens. The in vitro antibacterial activity of Tob-AgNPs clearly showed potential antibacterial property against Escherichia coli and Staphylococcus aureus compared with standard antibiotics. Thereby, these Tob-AgNPs may be potential candidates for the development of new bioactive materials for pathogenic microorganisms after further analysis to identify the active principles.

Graphical Abstract


Synthesis Tob-AgNPs Nitroaromatics Catalytic activity Antibacterial activity Bacterial pathogens 



Authors would like to thanks to Virtual University of Pakistan who supported the research work and necessary facilities at Biolab, Multan Campus.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Yasmeen Junejo
    • 1
  • Muhammad Safdar
    • 2
  • M. Asad Akhtar
    • 3
  • Muthupandian Saravanan
    • 4
  • Haseeb Anwar
    • 5
  • Muhammad Babar
    • 6
  • Rabia Bibi
    • 7
  • M. Tariq Pervez
    • 8
  • Tanveer Hussain
    • 9
  • Masroor E. Babar
    • 9
  1. 1.Department of BiologyVirtual University of Pakistan-MultanMultanPakistan
  2. 2.Department of Molecular BiologyVirtual University of Pakistan-MultanMultanPakistan
  3. 3.Department of Medical Biology, Faculty of Health SciencesUniversity of TromsøTromsøNorway
  4. 4.Department of Medical Microbiology and Immunology, School of Medicine, College of Health ScienceMekelle UniversityMekelleEthiopia
  5. 5.Department of PhysiologyGovernment College UniversityFaisalabadPakistan
  6. 6.Institute of Molecular Biology & Bio-TechnologyBahauddin Zakariya UniversityMultanPakistan
  7. 7.Department of Molecular BiologyVirtual University of Pakistan-RawalpindiRawalpindiPakistan
  8. 8.Department of Bioinformatics & Computational BiologyVirtual University of Pakistan-LahoreLahorePakistan
  9. 9.Department of Molecular BiologyVirtual University of Pakistan-LahoreLahorePakistan

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