Pyrene Based Fluorescent Turn-on Chemosensor for Sequential Detection of Fe3+ and Fe2+ Ions and its Application in Live Cell Imaging
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A simple pyrene-based “turn-on” chemosensor bearing isonizide namely 3-(pyren-1-yl methylene) pentane-2,4-diyldene di(isonicotinohydrazide) (PMPD) was designed and synthesized for detecting Fe3+and Fe2+ ions in HEPES buffer solution at pH 7.4 (DMSO: H2O (1:9 v/v)) medium. The probe shows conducive selectivity for Fe2+ and Fe3+ ions over the other competitive metal cations. The detection limit was found to be 1.67 μM for Fe3+ and 2.02 μM for Fe2+. The recognition mechanism of PMPD towards Fe3+ and Fe2+ has been examined in detail by absorption, emission, and ESI-MS studies. Moreover, “turn-on” fluorescence behavior of the probe was used to track iron ions in living cells.
KeywordsSchiff base Fluorescence probe Iron ions Cell imaging
The authors express their sincere thanks to Council of Scientific and Industrial Research (CSIR), New Delhi, India [Grant No. 01(2907)/17/EMR-II] for financial support.
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